FlyBase Allele Report: Dmel\RhoGEF2[04291]

General Information

Symbol

Dmel\RhoGEF2⁰⁴²⁹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0008055

Feature type

allele

Associated gene

Dmel\RhoGEF2

Associated Insertion(s)

P{PZ}RhoGEF2⁰⁴²⁹¹

Carried in Construct

Also Known As

RhoGEF2^l(2)04291, DRhoGEF2^l(2)04291, DrhoGEF2⁰⁴²⁹¹, l(2)04291

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Padash Barmchi et al., 2005)

Mutagen

P-element activity

(Hacker and Perrimon, 1998, Barrett et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{PZ}RhoGEF2⁰⁴²⁹¹

Cytology

Description

P{PZ} element insertion in an intron that interrupts the 5' UTR.

(Halsell et al., 2000)

P{PZ} insertion in the second intron.

(Hacker and Perrimon, 1998)

Insertion of a P{PZ} element within a 1.3kb intron at the 5' end of the gene.

(Barrett et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:17,028,424..17,028,424 [-]

(Sun et al., 2011, Vereshchagina et al., 2004, Halsell et al., 2000, Sotillos and Campuzano, 2000, Spradling et al., 1999, Hacker and Perrimon, 1998, Barrett et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

actin cytoskeleton | blastoderm stage | germline clone

(Padash Barmchi et al., 2005)

anterior midgut primordium | germline clone | maternal effect

(Hacker and Perrimon, 1998)

embryo | blastoderm stage | germline clone

(Padash Barmchi et al., 2005)

embryo | extended germ band stage | germline clone | maternal effect

(Hacker and Perrimon, 1998)

embryonic/first instar larval cuticle | germline clone | maternal effect | ventral

(Hacker and Perrimon, 1998)

embryonic/first instar larval cuticle | germline clone | ventral

(Padash Barmchi et al., 2005)

embryonic ventral epidermis | rescuable maternal effect

(Perrimon et al., 1996)

furrow canal | germline clone

(Wenzl et al., 2010, Padash Barmchi et al., 2005)

nucleus | blastoderm stage | germline clone

(Padash Barmchi et al., 2005)

pole cell & cytoskeleton | germ-line clone

(Padash Barmchi et al., 2005)

posterior midgut primordium | germline clone | maternal effect

(Hacker and Perrimon, 1998)

posterior spiracle primordium

(Simões et al., 2006)

primordial germ cell | germline clone

(Padash Barmchi et al., 2005)

ventral midline of embryo

(Fox and Peifer, 2007)

Detailed Description

Statement

Reference

Multinucleate cells are present in 100% of RhoGEF2⁰⁴²⁹¹ embryos during cellularisation, and disruptions in the furrow array are observed in these animals.

(Wenzl et al., 2010)

Embryos in the offspring of homozygous RhoGEF2⁰⁴²⁹¹ mothers display areas of decreased actin as well as areas of excessive actin associated with rings. Actin does not remain in apical caps during metaphase in these embryos. Actin pseudocleavage furrows often appear thickened in cross-sections in mutant embryos, but they do not show extension defects.

(Webb et al., 2009)

The two lateral rows of midline cells fail to form in mutant embryos, due to a failure of mesoderm internalisation.

(Fox and Peifer, 2007)

Embryos that are both maternally and zygotically mutant for RhoGEF2⁰⁴²⁹¹ show spiracle defects; 34% show a failure of spiracle invagination, 26% have lumen defects and 3.5% show both defects.

(Simões et al., 2006)

Embryos derived from RhoGEF2⁰⁴²⁹¹ germline clones contain a variable proportion of multinucleate cells.

(Großhans et al., 2005)

The following phenotypes are observed in embryos derived from RhoGEF2⁰⁴²⁹¹ germ-line clones (paternal rescue was not observed): 1) The depth of metaphase furrows at cycle 13 is more variable than wild type; some furrows break down or fail to invaginate which can cause adjacent mitotic spindles to collide and leads to the elimination of nuclei from the cortex. 2) During the slow phase of cellularization, actin rings appear rounded instead of hexagonal and are irregular in size, nuclei are wider than in wild type, and multinucleated actin rings are frequently observed. 3) When the cellularization front reaches the base of the nuclei, actin rings stay in proximity to each other rather than constricting. 4) During the subsequent fast phase of cellularization, actin rings are irregular in size and many multinucleated rings remain very large.

During cellularization, embryos derived from RhoGEF2⁰⁴²⁹¹ germ-line clones do not exhibit a decrease in the rate of membrane invagination but do show irregular radial movement of nuclei, with some nuclei remaining apical.

Embryos derived from RhoGEF2⁰⁴²⁹¹ germ-line clones show a hole in their ventral cuticle.

The following defects are observed during pole cell formation in embryos derived from RhoGEF2⁰⁴²⁹¹ germ-line clones: pole cells form but show an uneven distribution of actin; after nuclear division cycle 10, the pole cells fail to form independent cortical actin structures to separate them from the somatic nuclear layer; during cellularization, nuclei are eliminated from the cortex and accumulate in the yolk at the posterior pole, while the pole cells remain embedded in the somatic nuclear layer rather than sitting on top of the syncytium; finally, the invaginating cellularization front obliterates the majority of the pole cells in the RhoGEF2⁰⁴²⁹¹ mutants.

(Padash Barmchi et al., 2005)

Homozygotes die during late embryogenesis or early larval stages with no obvious phenotype. Embryos derived from germline mosaics develop with ventral open cuticles. Deep transverse folds form during germ band elongation. The posterior and anterior midguts fail to invaginate. Apical constriction of cells along the ventral midline at the beginning of gastrulation is severely disrupted. Cell shape changes appear to occur at random and no invagination is formed, phenotype suggests loss of an instructive signal.

(Hacker and Perrimon, 1998)

Germline clones produce eggs with patterning defects: ventral hypoderm is absent.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RhoGEF2⁰⁴²⁹¹, zip^Ebr has visible | dominant phenotype, suppressible by Mbs³

(Mizuno et al., 2002)

Enhancer of

Statement

Reference

RhoGEF2⁰⁴²⁹¹, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Suppressor of

Statement

Reference

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] is a suppressor | partially of abnormal cell polarity | recessive | somatic clone phenotype of flw^FP41

(Sun et al., 2011)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] is a suppressor of visible phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

RhoGEF2⁰⁴²⁹¹ is a suppressor of lethal | male phenotype of flw⁶

(Vereshchagina et al., 2004)

RhoGEF2⁰⁴²⁹¹ is a suppressor of visible phenotype of Rho1^GMR.PH

(Barrett et al., 1997)

Other

Statement

Reference

Rho1^E3.10/Rho1[+], RhoGEF2⁰⁴²⁹¹ has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^J3.8/Rho1[+], RhoGEF2⁰⁴²⁹¹ has visible | dominant phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹, Sb^63b/Sb[+] has visible phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹, Sb[+]/Sb⁷⁰ has visible phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹, Sb^sbd-1/Sb^sbd-201 has visible phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], Sb^63b has visible | dominant phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], Sb⁷⁰ has visible | dominant phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], zip^Ebr has visible phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], Sb^sbd-1/Sb^sbd-201 has visible phenotype

(Bayer et al., 2003)

Rho1^E3.10, RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] has visible phenotype

(Bayer et al., 2003)

Rho1^J3.8, RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] has visible phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹, zip[+]/zip^Ebr has visible | dominant phenotype

(Bayer et al., 2003, Halsell et al., 2000)

E(br)121^Ebr121, RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] has visible | dominant phenotype

(Ward et al., 2003)

E(br)121^Ebr121/E(br)121[+], RhoGEF2⁰⁴²⁹¹ has visible phenotype

(Ward et al., 2003)

E(br)444[+]/E(br)444^Ebr444, RhoGEF2⁰⁴²⁹¹ has visible phenotype

(Ward et al., 2003)

E(br)444^Ebr444, RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] has visible | dominant phenotype

(Ward et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], zip^Ebr has visible | dominant phenotype

(Mizuno et al., 2002)

RhoGEF2⁰⁴²⁹¹, zip^Ebr has visible | dominant phenotype

(Mizuno et al., 2002, Halsell et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

RhoGEF2⁰⁴²⁹¹ has actin cytoskeleton | blastoderm stage | germline clone phenotype, enhanceable by Df(3R)tll-e

(Padash Barmchi et al., 2005)

Suppressed by

Statement

Reference

RhoGEF2⁰⁴²⁹¹, zip^Ebr has wing phenotype, suppressible by Mbs³

(Mizuno et al., 2002)

Enhancer of

Statement

Reference

RhoGEF2⁰⁴²⁹¹, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Suppressor of

Statement

Reference

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] is a suppressor | partially of oocyte | somatic clone phenotype of flw^FP41

(Sun et al., 2011)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] is a suppressor of wing phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

RhoGEF2⁰⁴²⁹¹ is a suppressor of eye phenotype of Rho1^GMR.PH

(Barrett et al., 1997)

Other

Statement

Reference

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], dia² has amnioserosa | maternal effect phenotype

(Homem and Peifer, 2008)

Rho1^E3.10/Rho1[+], RhoGEF2⁰⁴²⁹¹ has leg phenotype

(Bayer et al., 2003)

Rho1^J3.8/Rho1[+], RhoGEF2⁰⁴²⁹¹ has leg phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹, Sb^sbd-1/Sb^sbd-201 has leg phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹, Sb^63b has leg phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹, Sb⁷⁰ has leg phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], Sb^63b has leg phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], Sb⁷⁰ has leg phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], Sb^sbd-1/Sb^sbd-201 has leg phenotype

(Bayer et al., 2003)

Rho1^E3.10, RhoGEF2⁰⁴²⁹¹ has leg phenotype

(Bayer et al., 2003)

Rho1^J3.8, RhoGEF2⁰⁴²⁹¹ has leg phenotype

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹, zip[+]/zip^Ebr has leg phenotype

(Bayer et al., 2003, Halsell et al., 2000)

RhoGEF2⁰⁴²⁹¹, zip^Ebr has leg phenotype

(Bayer et al., 2003, Halsell et al., 2000)

E(br)121^Ebr121, RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] has leg phenotype

(Ward et al., 2003)

E(br)121^Ebr121, RhoGEF2⁰⁴²⁹¹ has leg phenotype

(Ward et al., 2003)

E(br)444^Ebr444, RhoGEF2⁰⁴²⁹¹ has leg phenotype

(Ward et al., 2003)

E(br)444^Ebr444, RhoGEF2⁰⁴²⁹¹/RhoGEF2[+] has leg phenotype

(Ward et al., 2003)

RhoGEF2⁰⁴²⁹¹/RhoGEF2[+], zip^Ebr has wing phenotype

(Mizuno et al., 2002)

RhoGEF2⁰⁴²⁹¹, zip^Ebr has wing phenotype

(Mizuno et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of RhoGEF2⁰⁴²⁹¹ partially suppresses the oocyte polarity defects seen when the posterior follicle cells are mutant for flw^FP41.

(Sun et al., 2011)

The progeny of dia² RhoGEF2⁰⁴²⁹¹ double heterozygous females have abnormal protrusions extending from amnioserosa cells over the adjacent epidermis during dorsal closure.

(Homem and Peifer, 2008)

Embryos that are both maternally and zygotically mutant for RhoGEF2⁰⁴²⁹¹ and are also heterozygous or homozygous for Gef64C¹ show a mild increase in the frequency of spiracle defects compared to embryos that are both maternally and zygotically mutant for RhoGEF2⁰⁴²⁹¹ in a Gef64C⁺ background.

(Simões et al., 2006)

Embryos derived from RhoGEF2⁰⁴²⁹¹ germ-line clones that are also zygotically homozygous for Df(3R)tll-e resemble RhoGEF2⁰⁴²⁹¹ single mutants (derived form germ-line clones) during syncytial divisions. However, RhoGEF2⁰⁴²⁹¹, Df(3R)tll-e double mutants show a progressive deterioration in their actin networks during cellularization: they have breaks in actin rings more frequently than the single mutants; toward the end of the slow phase of cellularization, their actin network is severely fragmented with only isolated actin rings being found (although these isolated rings did not constrict prematurely, as is observed in Df(3R)tll-e embryos); and at later stages of cellularization, the actin network network is completely disrupted.

(Padash Barmchi et al., 2005)

RhoGEF2⁰⁴²⁹¹/+ suppresses the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is increased from 9% to 87%).

(Chen et al., 2004)

RhoGEF2⁰⁴²⁹¹ shows a weak interaction (5-24% of double heterozygotes have at least one malformed leg) with the following mutations: Sb^63b and Sb⁷⁰. br¹ fails to show a significant genetic interaction (assayed in terms of a malformed leg phenotype) with RhoGEF2⁰⁴²⁹¹. Sb^sbd-201/Sb^sbd-1 shows a weak interaction (5-24% of double mutants have at least one malformed leg) with the following mutations: RhoGEF2⁰⁴²⁹¹/+.

(Bayer et al., 2003)

The fraction of flies showing a malformed leg phenotype in at least one leg, for RhoGEF2⁰⁴²⁹¹ in double heterozygous combination with one of the following alleles is - Sb^Ebr20 : 1%, Sb^Ebr48 : 2%, Sb^Ebr228 : 4%, Sb^Ebr448 : 4%, Sb^Ebr536 : 2%, Sb^Ebr623 : 0%, Rho1^Ebr233 : 4%, Rho1^Ebr246 : 17%, bs^Ebr292 : 0%, E(br)24^Ebr24 : 2%, E(br)65^Ebr65 : 4%, E(br)155^Ebr155 : 6%, E(br)165^Ebr165 : 7%, E(br)333^Ebr333 : 9%, E(br)72^Ebr72 : 1%, E(br)121^Ebr121 : 49%, E(br)160^Ebr160 : 2%, E(br)187^Ebr187 : 2%, E(br)420^Ebr420 : 3% and E(br)444^Ebr444 : 36%.

(Ward et al., 2003)

38% of RhoGEF2⁰⁴²⁹¹/zip^Ebr double heterozygotes have a malformed leg phenotype.

(Halsell et al., 2000)

Suppresses the rough eye phenotype caused by Rho1^GMR.PH.

(Barrett et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RhoGEF2^11-3

(Bayer et al., 2003)

RhoGEF2^1.1

(Barrett et al., 1997)

RhoGEF2^4.1

(Barrett et al., 1997)

Rescued by

RhoGEF2⁰⁴²⁹¹ is rescued by RhoGEF2^RE.UAS/Scer\GAL4^{VP16.mat.αTub67C}

(Padash Barmchi et al., 2005)

Comments

The ventral open cuticle defect seen in embryos derived from RhoGEF2⁰⁴²⁹¹ germ-line clones is rescued when RhoGEF2^RE.Scer\UAS is expressed ubiquitously using Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

(Padash Barmchi et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11369

cn¹ P{PZ}RhoGEF2⁰⁴²⁹¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Excision of the P{PZ} insertion reverts the maternal effect demonstrating the insertion is responsible for the mutant phenotype.

(Hacker and Perrimon, 1998)

Precise excision of the P{PZ} element reverts the lethal phenotype and the genetic interaction with zip^Ebr.

(Halsell et al., 2000)

Complements: GstS1⁰⁴²²⁷. Complements: Sply⁰⁵⁰⁹¹. Complements: l(2)06253⁰⁶²⁵³. Complements: l(2)06655⁰⁶⁶⁵⁵. Complements: l(2)k11502^k11502. Complements: l(2)k15914^k15914.

(BDGP Project Members, 1994-1999)

Excision of the P{PZ} element demonstrates that the lethality and suppression of the Rho1^GMR.PH phenotype of RhoGEF2⁰⁴²⁹¹ is caused by the P{PZ} element insertion.

(Barrett et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

DRhoGEF21⁰⁴²⁹¹

(Mizuno et al., 2002)

DRhoGEF2⁰⁴²⁹¹

(Chang et al., 2016, Bayer et al., 2003)

DRhoGEF2^l(2)04291

(Simões et al., 2006, Padash Barmchi et al., 2005, Zhu et al., 2005, Hacker and Perrimon, 1998)

DrhoGEF2⁰⁴²⁹¹

(Chen et al., 2004)

Gef2^l(2)04291

(Massarwa et al., 2009)

RhoGEF204291

(Sun et al., 2011)

RhoGEF2⁰⁴²⁹¹

(Lin et al., 2022, Dent et al., 2019, Kong et al., 2019, Wenzl et al., 2010, Webb et al., 2009, Homem and Peifer, 2008, Christensen and Cook, 2007.10.29, Christensen et al., 2007.10.29, Fox and Peifer, 2007)

RhoGEF2^l(2)04291

(Dehapiot et al., 2020, Garcia De Las Bayonas et al., 2019, Kerridge et al., 2016, Wenzl et al., 2010)

l(2)04291

(Spradling et al., 1999, Barrett et al., 1997)

l(2)04291⁰⁴²⁹¹

rhoGEF2^l(2)04291

(Levayer et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (37)

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