From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2R)BSC359
Date: 	Mon, 29 Oct 2007  12:05:27  -0400  ( 16:05  GMT)
Isolation and characterization of Df(2R)BSC359
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC359 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}Cbp53E[f00876] and P{XP}GstS1[d07643]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
PBac{WH}Cbp53E[f00876]/P{XP}GstS1[d07643] males crossed to w[1118]; 
P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC359 from the segment of 
PBac{WH}Cbp53E[f00876] to the left of its FRT site and the segment of 
P{XP}GstS1[d07643] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2R)BSC359 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 53E4;53F8. It failed to complement RhoGEF2[04291] and Df(2R)ED2751.