FlyBase Allele Report: Dmel\RhoGEF2[RE.UAS]

General Information

Symbol

Dmel\RhoGEF2^RE.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0194297

Feature type

allele

Associated gene

Dmel\RhoGEF2

Associated Insertion(s)

Carried in Construct

P{UAS-DRhoGEF2.RE}

Key Links

Transgenic product class

cDNA

(Padash Barmchi et al., 2005)

wild_type

(Padash Barmchi et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Padash Barmchi et al., 2005)

wild_type

(Padash Barmchi et al., 2005)

Mutagen

in vitro construct

(Padash Barmchi et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-DRhoGEF2.RE}

Cytology

Description

UASt regulatory sequences drive expression of a XbaI fragment containing a RhoGEF2 cDNA corresponding to the RhoGEF2-RE transcript (made by joining a partial cDNA encompassing the region from a central EcoRI site to the 3' end of RhoGEF2 with a cDNA representing the RhoGEF2-RE transcript from the same EcoRI site to the 5' end).

(Padash Barmchi et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

RhoGEF2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Raf^UAS.F179

(Khoo et al., 2013)

CEA with Ras85D^G12V.UAS

(Khoo et al., 2013)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye-antennal disc | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU

(Khoo et al., 2013)

eye photoreceptor cell | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Detailed Description

Statement

Reference

Eye-antennal disc clones expressing RhoGEF2^RE.Scer\UAS under the control of Scer\GAL4^tub.PU exhibit decreased photoreceptor differentiation, small clone size, and also show overgrowth over time as compared to non-clonal tissue.

(Khoo et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Rho1^GD4726, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, suppressible | somatic clone by Rho1^GD4726, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Rok^GD1522, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, suppressible | somatic clone by Rok^GD1522, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by zip^GD1566, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, suppressible | somatic clone by zip^GD1566, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

NOT suppressed by

Statement

Reference

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Rac1^N17.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Rac1^GD17411, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Rac1^J11/Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1[+]

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, non-suppressible | somatic clone by dia^GD9442, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, non-suppressible | somatic clone by LIMK1^GD9586, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, non-suppressible | somatic clone by meng^GD7239, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Other

Statement

Reference

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype

(Khoo et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | third instar larval stage | somatic clone phenotype, enhanceable | somatic clone by Raf^UAS.F179, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | third instar larval stage | somatic clone phenotype, enhanceable | somatic clone by Raf^UAS.F179, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | third instar larval stage | somatic clone phenotype, enhanceable | somatic clone by Ras85D^G12V.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | third instar larval stage | somatic clone phenotype, enhanceable | somatic clone by Ras85D^G12V.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Suppressed by

Statement

Reference

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Rho1^GD4726, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Rho1^GD4726, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Rok^GD1522, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Rok^GD1522, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by zip^GD1566, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by zip^GD1566, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Rho1^GD4726, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Rho1^GD4726, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Rok^GD1522, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by zip^GD1566, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

NOT suppressed by

Statement

Reference

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1^J11/Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1[+]

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1^J11/Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1[+]

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by dia^GD9442, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by LIMK1^GD9586, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by meng^GD7239, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by dia^GD9442, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by LIMK1^GD9586, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by meng^GD7239, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1^N17.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1^GD17411, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1^N17.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1^GD17411, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Raf^{Scer\UAS.F179} or Ras85D^{G12V.Scer\UAS} enhances the decreased photoreceptor differentiation seen in eye-antennal disc clones expressing RhoGEF2^RE.Scer\UAS under the control of Scer\GAL4^tub.PU. These clones show overgrowth, similar to those expressing only RhoGEF2^RE.Scer\UAS, but also show large undifferentiated clonal masses in the basal regions of the posterior region of the eye disc. Larvae containing clones expressing both RhoGEF2^RE.Scer\UAS and Raf^{Scer\UAS.F179} under the control of Scer\GAL4^tub.PU exhibit an extended larval period, as compared with controls.

Co-expression of Rho1^GD4726, zip^GD1566 or Rok^GD1522 suppresses the small clone size, differentiation and cell morphology defects seen in eye-antennal disc clones expressing RhoGEF2^RE.Scer\UAS under the control of Scer\GAL4^tub.PU.

Co-expression of Rho1^GD4726, zip^GD1566 or Rok^GD1522 suppresses the formation of undifferentiated clonal masses and partially suppresses the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2^RE.Scer\UAS and Raf^{Scer\UAS.F179} under the control of Scer\GAL4^tub.PU, and also suppresses the developmental delay shown by larvae containing these clones.

Co-expression of Rac1^N17.Scer\UAS, Rac1^GD17411, CG11221^GD7239, or dia^GD9442 fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2^RE.Scer\UAS and Ras85D^{G12V.Scer\UAS} under the control of Scer\GAL4^tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

Co-expression of LIMK1^GD9586 fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2^RE.Scer\UAS and Raf^{Scer\UAS.F179} under the control of Scer\GAL4^tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

The presence of Rac2^Δ/+, Rac1^J11/+ and Mtl^Δ/+ mutations fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2^RE.Scer\UAS and Ras85D^{G12V.Scer\UAS} under the control of Scer\GAL4^tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

(Khoo et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

RhoGEF2^RE.UAS/Scer\GAL4^{VP16.mat.αTub67C} rescues RhoGEF2⁰⁴²⁹¹

(Padash Barmchi et al., 2005)

Comments

The ventral open cuticle defect seen in embryos derived from RhoGEF2⁰⁴²⁹¹ germ-line clones is rescued when RhoGEF2^RE.Scer\UAS is expressed ubiquitously using Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

(Padash Barmchi et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RhoGEF2^RE.Scer\UAS

RhoGEF2^RE.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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