FlyBase Allele Report: Dmel\zip[GD1566]

General Information

Symbol

Dmel\zip^GD1566

Species

D. melanogaster

Name

FlyBase ID

FBal0210938

Feature type

allele

Associated gene

Dmel\zip

Associated Insertion(s)

Carried in Construct

P{GD1566}

Also Known As

UAS-zip^RNAi, zip RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1566}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1566

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by RhoGEF2^RE.UAS, Raf^UAS.F179

(Khoo et al., 2013)

exacerbates carcinoma

modeled by scrib¹

(Kong et al., 2022)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 7

modeled by Hsap\CHMP2B^Intron5.UAS

(Jun et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

lethal, with Scer\GAL4^Bx-MS1096

(Dietzl et al., 2007)

lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal, with Scer\GAL4^pnr-MD237

(Dietzl et al., 2007)

lethal, with Scer\GAL4^tsh-md621

(Petzoldt et al., 2012)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of zip^GD1566 under the control of Scer\GAL4^hth-GETDB in the larval antennal disc leads to disorganization and a breakdown of the field boundary at the A1 fold, in contrast to controls, and mislocalized cells are maintained and integrated in the epithelial sheet.

(Ku and Sun, 2017)

Eye-antennal disc clones expressing zip^GD1566 under the control of Scer\GAL4^tub.PU exhibit a small reduction in size as compared to wild type clones, along with some defects in differentiation and cell morphology.

(Khoo et al., 2013)

Expression of zip^GD1566 lessens the invasiveness of Ras85D^V12.Scer\UAS scrib¹ tumours and improves pupation rate.

(Külshammer and Uhlirova, 2013)

Flies expressing zip^GD1566 under the control of Scer\GAL4^Abd-B-LDN show partial rotation of the male genitalia.

(Petzoldt et al., 2012)

Expression of zip^GD1566 under the control of Scer\GAL4^Act.PU in clones in the eye disc results in differentiation defects without inducing a cell invasion phenotype.

(Jiang et al., 2011)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an extreme increase in neuroblast size in the larval brain, without loss of neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in extreme defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing zip^GD1566 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1566

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

zip^GD1566, Scer\GAL4^GMR.PF is a suppressor | partially of visible | adult stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Jun et al., 2023)

zip^GD1566, Scer\GAL4^GMR.PF is a suppressor | partially of melanotic necrosis | adult stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Jun et al., 2023)

zip^GD1566, Scer\GAL4^Tub.PU is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

zip^GD1566, Scer\GAL4^Tub.PU is a suppressor | somatic clone of abnormal developmental rate | somatic clone | larval stage phenotype of Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

zip^GD1566, Scer\GAL4^T80 is a suppressor of abnormal cell migration | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^T80, scrib¹

(Külshammer and Uhlirova, 2013)

zip^GD1566, Scer\GAL4^T80 is a suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^T80, scrib¹

(Külshammer and Uhlirova, 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

zip^GD1566/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, chb^UAS.GFP

(Lowery et al., 2010)

Suppressor of

Statement

Reference

zip^GD1566, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Jun et al., 2023)

zip^GD1566, Scer\GAL4^Tub.PU is a suppressor | partially | somatic clone of eye photoreceptor cell | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

zip^GD1566, Scer\GAL4^Tub.PU is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

zip^GD1566, Scer\GAL4^Tub.PU is a suppressor | somatic clone of eye photoreceptor cell | somatic clone | third instar larval stage phenotype of RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

zip^GD1566, Scer\GAL4^Tub.PU is a suppressor | partially | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Scer\GAL4^Act.PU/zip^GD1566 is a suppressor | somatic clone of eye disc | somatic clone phenotype of sds22^Δ2.8

(Jiang et al., 2011)

NOT Suppressor of

Statement

Reference

zip^GD1566, Scer\GAL4^Tub.PU is a non-suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

zip^GD1566, Scer\GAL4^Tub.PU is a non-suppressor | somatic clone of eye photoreceptor cell | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of zip^GD1566 suppresses the small clone size, differentiation and cell morphology defects seen in eye-antennal disc clones expressing RhoGEF2^RE.Scer\UAS under the control of Scer\GAL4^tub.PU.

Co-expression of zip^GD1566 suppresses the formation of undifferentiated clonal masses and partially suppresses the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2^RE.Scer\UAS and Raf^{Scer\UAS.F179} under the control of Scer\GAL4^tub.PU, and also suppresses the developmental delay shown by larvae containing these clones.

Co-expression of zip^GD1566 fails to suppress the ectopic differentiation of seen in eye-antennal disc clones expressing Raf^{Scer\UAS.F179} under the control of Scer\GAL4^tub.PU.

(Khoo et al., 2013)

The cell invasion phenotype (mislocalisation to the optic stalk) shown by sds22^Δ2.8 eye disc cells is suppressed if the cells are also expressing zip^GD1566 under the control of Scer\GAL4^Act.PU.

(Jiang et al., 2011)

Knockdown of zip through coexpression of zip^GD1566 with chb^{Scer\UAS.T:Avic\GFP} in the developing eye under the control of Scer\GAL4^GMR.PF leads to a severe eye phenotype.

(Lowery et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v7819