FlyBase Allele Report: Dmel\chb[UAS.GFP]

General Information

Symbol

Dmel\chb^UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0159409

Feature type

allele

Associated gene

Dmel\chb

Associated Insertion(s)

Carried in Construct

P{UAS-chb.GFP}

Also Known As

UAS-Orbit-GFP

Key Links

Transgenic product class

wild_type

(Lee et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lee et al., 2004)

Mutagen

in vitro construct

(Lee et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-chb.GFP}

Cytology

Description

UAS regulatory sequences drive expression of chb tagged with GFP.

(Lee et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

chb

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Lowery et al., 2010)

abnormal neuroanatomy | P-stage | RU486 conditional, with Scer\GAL4^GSG2295

(Bu et al., 2022)

Phenotype Manifest In

dendrite | P-stage | RU486 conditional, with Scer\GAL4^GSG2295

(Bu et al., 2022)

eye, with Scer\GAL4^GMR.PF

(Lowery et al., 2010)

fascicle, with Scer\GAL4^elav.PLu

(Lowery et al., 2010)

larval dorsal multidendritic neuron ddaC | P-stage | RU486 conditional, with Scer\GAL4^GSG2295

(Bu et al., 2022)

Detailed Description

Statement

Reference

Expression of chb^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

Expression of chb^{Scer\UAS.T:Avic\GFP} in post-mitotic neurons under the control of Scer\GAL4^elav.PLu results in abnormal longitudinal fascicles, with deflections in pathfinding and some segments missing fascicles, primarily in the outer fascicle.

(Lowery et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, chb^UAS.GFP has abnormal neuroanatomy phenotype, enhanceable by Df(3R)Exel7328

(Lowery et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Df(3R)Exel7328

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Df(2R)Exel7128

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by pnut^c04668

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by trbl^d03175

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by wnd^f00690

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Mekk1^d01115

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by tra2^d10032

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by aop^d08252

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by E(bx)^d02664

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by slv^d07339

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by sano^d10230

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by zip^GD1566/Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Df(3R)Exel6181

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by msps^P/Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Scer\GAL4^GMR.PF/msps^c03283

(Lowery et al., 2010)

Scer\GAL4^elav.PLu, chb^UAS.GFP has fascicle phenotype, enhanceable by Df(3R)Exel7328

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Df(3L)Exel6130

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Df(2L)Exel6027

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Df(2R)Exel7094

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by Df(2R)Exel6067

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by msps^c03283

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by shot^d00747

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, enhanceable by shot^d03296

(Lowery et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(2R)Exel6057

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(2L)Exel6009

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(2R)Exel7137

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by msps^d03376

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by capu^d10743

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Cam^d09785b

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Su(dx)^f02193

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Dab^d11255

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by wrd^c00535

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by CG31957^f05539

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by dve^d05100

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by shn^d05100

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(2R)Exel6061

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Su(z)2^f07746

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Phgdh^d01875

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Vha36-2^d09785b

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Npc2a^d04217

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by RfC38^f07177

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by CG42524^f00129

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by bip1^d05100

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by CG34021^d09785a

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by sip2^d04917

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by msps^d03376/Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(3L)Exel9009

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(2L)Exel6006

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(3R)Exel6178

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(2R)Exel6284

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(2R)Exel6059

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(2R)Exel6062

(Lowery et al., 2010)

Scer\GAL4^GMR.PF, chb^UAS.GFP has eye phenotype, suppressible by Df(3L)Exel6084

(Lowery et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

A CLIP-190^c02410 mutant background suppresses the rough eye phenotype found when chb^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^GMR.PF.

Co-expression of Abl^Scer\UAS.cFa enhances the chb^{Scer\UAS.T:Avic\GFP} mutant eye phenotype when both transgenes are expressed under the control of Scer\GAL4^GMR.PF.

Knockdown of zip through coexpression of zip^GD1566 with chb^{Scer\UAS.T:Avic\GFP} in the developing eye under the control of Scer\GAL4^GMR.PF leads to a severe eye phenotype.

Co-expression of msps^d03376 with chb^{Scer\UAS.T:Avic\GFP}, both under the control of Scer\GAL4^GMR.PF, leads to a strong suppression of the chb^{Scer\UAS.T:Avic\GFP} rough eye phenotype.

Co-expression of msps^P with chb^{Scer\UAS.T:Avic\GFP}, both under the control of Scer\GAL4^GMR.PF, leads to a strong enhancement of the chb^{Scer\UAS.T:Avic\GFP} rough eye phenotype.

Co-expression of msps^c03283 with chb^{Scer\UAS.T:Avic\GFP}, both under the control of Scer\GAL4^GMR.PF, leads to a strong enhancement of the chb^{Scer\UAS.T:Avic\GFP} rough eye phenotype.

Expression of chb^{Scer\UAS.T:Avic\GFP} in post-mitotic neurons under the control of Scer\GAL4^elav.PLu in a Df(3R)Exel7328 heterozygous background leads to severe defects in longitudinal axon fascicle formation, with significantly higher penetrance than in either single mutant.

(Lowery et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

chb^UAS.GFP/Scer\GAL4^srp.Hemo rescues chb²

(Stramer et al., 2010)

chb^UAS.GFP/Scer\GAL4^elav.PLu rescues chb⁴/chb²

(Lee et al., 2004)

Comments

Expression of chb^{Scer\UAS.T:Avic\GFP} in hemocytes under the control of Scer\GAL4^srp.Hemo rescues microtubule arm formation in chb² mutants. In addition, expression of chb^{Scer\UAS.T:Avic\GFP} restores contact repulsion between hemocytes resulting in wild-type levels of contact time between cells.

(Stramer et al., 2010)

The ectopic crossing of the midline by central nervous system axons that is seen in chb²/chb⁴ embryos is rescued by expression of chb^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PLu.

(Lee et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

chb^{Scer\UAS.T:Avic\GFP}

chb^UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (4)

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