FlyBase Allele Report: Dmel\chb[4]

General Information

Symbol

Dmel\chb⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0104955

Feature type

allele

Associated gene

Dmel\chb

Associated Insertion(s)

Carried in Construct

Also Known As

orbit⁴

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Inoue et al., 2000)

Mutagen

Delta2-3 transposase

(Inoue et al., 2000)

Progenitor genotype

P{lacW}chb^S068607

(Inoue et al., 2000)

chb^S068607

(Inoue et al., 2000)

Caused by aberration

Df(3L)chb⁴

Cytology

Description

Imprecise excision of the P{lacW} element, resulting in a deletion extending from within the asparagine-synthetase gene to within the chb gene.

(Inoue et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendrite | larval stage | somatic clone

(Bu et al., 2022)

dendrite | P-stage | somatic clone

(Bu et al., 2022)

embryonic/larval central nervous system | larval stage (with chb²)

(Inoue et al., 2000)

larval dorsal multidendritic neuron ddaC (with chb⁴⁶⁴)

(Bu et al., 2022)

larval dorsal multidendritic neuron ddaC | larval stage | somatic clone

(Bu et al., 2022)

larval dorsal multidendritic neuron ddaC | P-stage | somatic clone

(Bu et al., 2022)

larval intersegmental nerve

(Lee et al., 2004)

microtubule (with chb⁴⁶⁴)

(Bu et al., 2022)

mitotic cell cycle

(Inoue et al., 2000)

mitotic metaphase

(Inoue et al., 2000)

pCC neuron

(Lee et al., 2004)

presumptive embryonic/larval central nervous system

(Lee et al., 2004)

presumptive embryonic/larval central nervous system (with chb²)

(Lee et al., 2004)

wing disc | larval stage (with chb²)

(Inoue et al., 2000)

Detailed Description

Statement

Reference

Homozygous and chb²/chb⁴ embryos show ectopic crossing of the midline by axons in the central nervous system. pCC axons often orient towards the midline in mutant embryos (in contrast to wild type where pCC axons extend slightly away from the midline) and sometimes crosses the midline to meet its contralateral homologue. Mutant embryos occasionally show premature arrest of the ISNb axon, at the muscle 13 cleft.

(Lee et al., 2004)

Mitotic defects are seen in the cells of the larval central nervous system in chb⁴/chb^S068607 flies. Polyploid metaphase figures (15%), diploid metaphase figures with hypercondensed chromosomes (4%) and circular metaphase figures (4%) are seen. The metaphase to anaphase ratio and the mitotic index is increased compared to wild type. Third instar chb⁴/chb² larvae lack imaginal discs and have a small central nervous system. More than 80% of total metaphase figures are polyploid, and more than 30% of polyploid figures have greater than an 8N chromosome complement. Circular metaphase figures and anaphase figures cannot be detected.

(Inoue et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

chb⁴ has abnormal neuroanatomy phenotype, enhanceable by Abl²

(Lee et al., 2004)

chb⁴, robo1¹ has abnormal neuroanatomy phenotype, enhanceable by robo2⁸

(Lee et al., 2004)

NOT Enhanced by

Statement

Reference

chb⁴, robo1¹ has abnormal neuroanatomy phenotype, non-enhanceable by robo3¹

(Lee et al., 2004)

Enhancer of

Statement

Reference

chb⁴ is an enhancer of abnormal neuroanatomy phenotype of Abl²

(Lee et al., 2004)

Suppressor of

Statement

Reference

chb⁴/chb⁴ is a suppressor | partially of abnormal neuroanatomy phenotype of Abl^UAS.cFa, Scer\GAL4^elav.PLu

(Lee et al., 2004)

Other

Statement

Reference

chb⁴, sli²/sli[+] has abnormal neuroanatomy phenotype

(Lee et al., 2004)

chb⁴, robo1¹ has abnormal neuroanatomy phenotype

(Lee et al., 2004)

chb⁴/chb[+], robo1¹ has abnormal neuroanatomy phenotype

(Lee et al., 2004)

chb⁴, sli² has abnormal neuroanatomy phenotype

(Lee et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

chb⁴ has larval intersegmental nerve phenotype, enhanceable by Abl²

(Lee et al., 2004)

chb⁴ has embryonic/larval neuron phenotype, enhanceable by Abl²

(Lee et al., 2004)

chb⁴, robo1¹ has embryonic/larval neuron phenotype, enhanceable by robo2⁸

(Lee et al., 2004)

NOT Enhanced by

Statement

Reference

chb⁴, robo1¹ has embryonic/larval neuron phenotype, non-enhanceable by robo3¹

(Lee et al., 2004)

Enhancer of

Statement

Reference

chb⁴ is an enhancer of larval intersegmental nerve phenotype of Abl²

(Lee et al., 2004)

chb⁴ is an enhancer of embryonic/larval neuron phenotype of Abl²

(Lee et al., 2004)

Suppressor of

Statement

Reference

chb⁴/chb⁴ is a suppressor | partially of larval intersegmental nerve phenotype of Abl^UAS.cFa, Scer\GAL4^elav.PLu

(Lee et al., 2004)

Other

Statement

Reference

chb⁴, sli²/sli[+] has embryonic/larval neuron phenotype

(Lee et al., 2004)

chb⁴, robo1¹ has embryonic/larval neuron phenotype

(Lee et al., 2004)

chb⁴/chb[+], robo1¹ has embryonic/larval neuron phenotype

(Lee et al., 2004)

chb⁴, sli² has embryonic/larval neuron phenotype

(Lee et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of axons ectopically crossing the midline in chb⁴ Abl² double homozygous embryos is substantially increased compared to the frequency seen in either single homozygote. chb⁴ Abl² double homozygous embryos show an increased frequency of premature arrest of the ISNb axon compared to either single homozygote, with the arrest being seen at both the muscle 6/7 and the muscle 13 choice points. sli² chb⁴ double heterozygous embryos have axons ectopically crossing the midline. capt¹⁰ chb⁴ double heterozygous embryos do not show a significant increase in axons ectopically crossing the midline compared to controls. robo¹ chb⁴ double heterozygous embryos have axons ectopically crossing the midline. Heterozygosity for lea^robo2-8 enhances the frequency of axons ectopically crossing the midline that is seen in robo¹ chb⁴ double heterozygous embryos. Heterozygosity for robo3¹ does not enhance the frequency of axons ectopically crossing the midline that is seen in robo¹ chb⁴ double heterozygous embryos. The ISNb bypass phenotype caused by expression of Abl^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu is reduced by chb⁴/chb⁴.