FlyBase Allele Report: Dmel\robo3[1]

General Information

Symbol

Dmel\robo3¹

Species

D. melanogaster

Name

FlyBase ID

FBal0121547

Feature type

allele

Associated gene

Dmel\robo3

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Rajagopalan et al., 2000)

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A. Mutation is in the splice acceptor site of the 6th intron.

(Rajagopalan et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult mushroom body gamma-lobe

(Nicolas and Preat, 2005)

adult s-LNv neuron | adult stage

(Oliva et al., 2016)

antennal lobe glomerulus | ectopic | somatic clone

(Jhaveri et al., 2004)

antennal lobe glomerulus DM2 | somatic clone

(Jhaveri et al., 2004)

axon | adult stage

(Oliva et al., 2016)

chordotonal organ & embryo & nerve terminal

(Zlatic et al., 2003)

ellipsoid body

(Nicolas and Preat, 2005)

embryonic longitudinal glial cell

(Kinrade and Hidalgo, 2004)

germline cell | embryonic stage (with Df(2L)Exel7006)

(Weyers et al., 2011)

gonad | embryonic stage (with Df(2L)Exel7006)

(Weyers et al., 2011)

intermediate longitudinal fascicle

(Evans and Bashaw, 2010, Spitzweck et al., 2010, Kinrade and Hidalgo, 2004, Zlatic et al., 2003)

intermediate longitudinal fascicle, with Scer\GAL4^12.1, robo3^UAS.cSa

(Zlatic et al., 2003)

larval longitudinal connective

(Evans and Bashaw, 2010, Rajagopalan et al., 2000)

medial lobe of adult mushroom body

(Nicolas and Preat, 2005)

medial longitudinal fascicle

(Kinrade and Hidalgo, 2004, Zlatic et al., 2003)

medial longitudinal fascicle, with Scer\GAL4^12.1, robo3^UAS.cSa

(Zlatic et al., 2003)

neurite | adult stage | ectopic | female | somatic clone

(Ito et al., 2016)

olfactory neuron & antennal lobe | somatic clone

(Jhaveri et al., 2004)

vertical lobe of adult mushroom body

(Nicolas and Preat, 2005)

Detailed Description

Statement

Reference

robo3¹/robo3¹ MARCM clones (in Scer\GAL4^fru-NP0021 neurons) develop a male-specific ipsilateral ectopic neurite in female brains at a low frequency (no effect in controls or mutant male clones).

(Ito et al., 2016)

The adult sLNv neurons of robo3¹ homozygotes exhibit significantly longer axonal projections compared to controls.

(Oliva et al., 2016)

Examination of the optic lobes of homozygous robo3¹ mutants does not reveal any defects.

(Pappu et al., 2011)

robo3¹/Df(2L)Exel7006 embryos exhibit gonad compaction defects and germ cell ensheathment defects.

(Weyers et al., 2011)

In robo3¹ mutants, the intermediate Fas2-positive tract shifts towards the midline and joins the medial pathway. Thus, only two distinct Fas2-positive bundles (medial and lateral) are detectable in these embryos. The Scer\GAL4^ap-md544-expressing axons are found in their wild-type position directly adjacent to the medial Fas2-tract.

(Evans and Bashaw, 2010)

Homozygous embryos show a highly penetrant medial shift of the intermediate Fas2-positive longitudinal fascicle.

(Spitzweck et al., 2010)

46% of homozygous embryos hatch into first instar larvae, 29.5% of the embryos reach the pupal stage and 27.5% of the embryos eclose into adults.

(Bhat et al., 2007)

Homozygotes have have defects in the ellipsoid body.

Homozygotes show defects in the mushroom body; 100% have the dorsal lobes close to the midline, 73% show crosswise projection of the γ fiber and 48% lack the median projection.

(Nicolas and Preat, 2005)

robo3¹/robo3¹ DM2 glomerulus innervating neurons, produced by mitotic recombination in a robo3¹/+ background, often project to ectopic sites in the antennal lobes, rather than to the DM2 glomerulus. The erroneously placed terminals form large, irregular "glomerular-like" structures that do not correspond in shape or position to those previously identified in wild-type animals.

(Jhaveri et al., 2004)

In embryonic stage 16 robo3¹ mutants, the medial and intermediate axonal fascicles fuse, with some longitudinal glia lining up over the fused fascicles, whereas some remain laterally together with remaining lateral axons (100% penetrance).

(Kinrade and Hidalgo, 2004)

Homozygous larvae have no detectable defect in distal cell neuron positioning in the visual system.

(Tayler et al., 2004)

Homozygous embryos do not show defects in lch5 or dorsal cluster sensory axon growth.

(Parsons et al., 2003)

The ch neuron projections in robo3¹/+ embryos are normal. The ch neuron projections in homozygous robo3¹ embryos are shifted to the medial, rather than the intermediate longitudinal fascicle, as are most of the Fas2 fascicles. The dbd neuron projection is unchanged. When robo3^Scer\UAS.cSa is expressed in the PNS by Scer\GAL4^12.1 in a robo3¹ mutant the medial fascicle is increased at the expense of the intermediate fascicle, as for robo3¹, but the ch neuron projections remain with the intermediate longitudinal fascicle.

(Zlatic et al., 2003)

Axonal projections in the visual system are not disrupted in mutants. Homozygous embryos show defects in the arrangement of Fas2-expressing fascicles. The intermediate fascicle shifts medially to fuse with the medial fascicle in 98.8% of embryos. 25.2% of embryos show breaks in the lateral fascicle. The axons of the "Sema2b" neurons (which express the marker Btau\MAPT^{Sema-2b.T:Hsap\MYC}), but not the cell bodies, are shifted much closer to the midline in mutant embryos.

(Rajagopalan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

robo3¹ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by robo1¹

(Evans and Bashaw, 2010)

robo1^unspecified, robo3¹ has abnormal neuroanatomy phenotype, enhanceable by Abl²/Abl[+]

(Wills et al., 2002)

NOT Enhanced by

Statement

Reference

robo1¹, robo3[+]/robo3¹ has abnormal neuroanatomy | recessive phenotype, non-enhanceable by capt¹⁰/capt[+]

(Wills et al., 2002)

NOT Enhancer of

Statement

Reference

robo3¹ is a non-enhancer of abnormal neuroanatomy phenotype of chb⁴, robo1¹

(Lee et al., 2004)

NOT Suppressor of

Statement

Reference

robo3¹ is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2010)

robo1¹/robo3¹ is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2010)

Other

Statement

Reference

robo1¹, robo3¹ has abnormal neuroanatomy phenotype

(Orgogozo et al., 2004)

robo1¹, robo3[+]/robo3¹ has abnormal neuroanatomy | recessive phenotype

(Wills et al., 2002)

robo1^unspecified, robo3¹ has abnormal neuroanatomy phenotype

(Wills et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

robo3¹ has larval longitudinal connective phenotype, enhanceable by robo1¹

(Evans and Bashaw, 2010)

robo3¹ has chordotonal organ & embryo & nerve terminal phenotype, enhanceable by sli²/sli[+]

(Zlatic et al., 2003)

robo1^unspecified, robo3¹ has presumptive embryonic/larval central nervous system phenotype, enhanceable by Abl²/Abl[+]

(Wills et al., 2002)

NOT Enhanced by

Statement

Reference

Df(2L)Exel7006/robo3¹ has gonad | embryonic stage phenotype, non-enhanceable by robo2⁴/robo2⁸

(Weyers et al., 2011)

Df(2L)Exel7006/robo3¹ has germline cell | embryonic stage phenotype, non-enhanceable by robo2⁴/robo2⁸

(Weyers et al., 2011)

Df(2L)Exel7006/robo3¹ has gonad | embryonic stage phenotype, non-enhanceable by robo1²/robo1¹

(Weyers et al., 2011)

Df(2L)Exel7006/robo3¹ has germline cell | embryonic stage phenotype, non-enhanceable by robo1²/robo1¹

(Weyers et al., 2011)

robo1¹, robo3[+]/robo3¹ has presumptive embryonic/larval central nervous system phenotype, non-enhanceable by capt¹⁰/capt[+]

(Wills et al., 2002)

robo3¹ has presumptive embryonic/larval nervous system phenotype, non-enhanceable by robo1¹

(Rajagopalan et al., 2000)

robo3¹ has embryonic/larval muscle system phenotype, non-enhanceable by robo1¹

(Rajagopalan et al., 2000)

NOT Enhancer of

Statement

Reference

robo3¹/robo3¹ is a non-enhancer of gonad | embryonic stage phenotype of robo1²/robo1¹

(Weyers et al., 2011)

robo3¹/robo3¹ is a non-enhancer of germline cell | embryonic stage phenotype of robo1²/robo1¹

(Weyers et al., 2011)

robo3¹/robo3¹ is a non-enhancer of gonad | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo3¹/robo3¹ is a non-enhancer of gonadal sheath proper primordium | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo3¹/robo3¹ is a non-enhancer of germline cell | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo3¹ is a non-enhancer of embryonic/larval neuron phenotype of chb⁴, robo1¹

(Lee et al., 2004)

robo3¹ is a non-enhancer of presumptive embryonic/larval nervous system phenotype of robo1¹

(Rajagopalan et al., 2000)

robo3¹ is a non-enhancer of embryonic/larval muscle system phenotype of robo1¹

(Rajagopalan et al., 2000)

NOT Suppressor of

Statement

Reference

robo3¹ is a non-suppressor of larval longitudinal connective phenotype of Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2010)

robo1¹/robo3¹ is a non-suppressor of larval longitudinal connective phenotype of Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2010)

Other

Statement

Reference

robo1¹, robo3¹ has larval abdominal ventral cluster group multidendritic neuron phenotype

(Orgogozo et al., 2004)

robo1¹, robo3[+]/robo3¹ has presumptive embryonic/larval central nervous system phenotype

(Wills et al., 2002)

robo1^unspecified, robo3¹ has presumptive embryonic/larval central nervous system phenotype

(Wills et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

A robo3¹ genetic background does not affect the ability of ectopic Scer\GAL4^ap-md544>lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} to force the Scer\GAL4^ap-md544-expressing axons to extreme lateral positions.

In robo3¹, robo¹ double mutants, the intermediate Fas2-positive tract fuses with the medial tract, and this thick fascicle crosses the midline. As in robo¹ mutants, the Scer\GAL4^ap-md544-expressing axons follow this fascicle across the midline. The lateral Fas2-positive tract remains in robo3¹, robo¹ double mutants.

Ectopic Scer\GAL4^ap-md544>lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} is able to direct the Scer\GAL4^ap-md544-expressing axons to extreme lateral positions even in a robo3¹, robo¹ mutant genetic background.

(Evans and Bashaw, 2010)

Heterozygosity for robo3¹ does not enhance the frequency of axons ectopically crossing the midline that is seen in robo¹ chb⁴ double heterozygous embryos.

(Lee et al., 2004)

robo¹ robo3¹ double mutant embryos do not show defects in the position of the vmd1a neuron of the ventral multidendritic neuron cluster in the abdomen; a single vmd1a neuron is seen in each hemisegment.

(Orgogozo et al., 2004)

The ch neurons in sli²/+ robo3¹/+ embryos frequently show abnormal projections.

(Zlatic et al., 2003)

capt¹⁰ robo3¹ double heterozygotes show little if any midline crossing defects in the embryonic central nervous system. One copy of capt¹⁰ does not enhance the frequency of midline crossing defects seen in robo¹ robo3¹ double mutant heterozygotes. The frequency of ectopic crossing of the midline by axons in the central nervous system seen in robo^unspecified robo3¹ embryos is increased by Abl²/+.

(Wills et al., 2002)

robo¹/robo3¹ show an embryonic nervous system that is merely additive.

(Rajagopalan et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

robo3¹ is partially rescued by Scer\GAL4^12.1/robo3^UAS.cSa

(Zlatic et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66885

y¹ w^*; robo3¹/CyO, P{en1}wg^en11

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

robo3¹

(Evans, 2017, Ito et al., 2016, Oliva et al., 2016, Santiago et al., 2014, Sekine et al., 2013, Evans and Bashaw, 2012, Pappu et al., 2011, Weyers et al., 2011, Evans and Bashaw, 2010, Spitzweck et al., 2010, Bhat et al., 2007, Nicolas and Preat, 2005)

Name Synonyms

Secondary FlyBase IDs

References (26)

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