FlyBase Allele Report: Dmel\chb[2]

General Information

Symbol

Dmel\chb²

Species

D. melanogaster

Name

FlyBase ID

FBal0104957

Feature type

allele

Associated gene

Dmel\chb

Associated Insertion(s)

Carried in Construct

Also Known As

orbit²

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Inoue et al., 2000)

Mutagen

Delta2-3 transposase

(Inoue et al., 2000)

Progenitor genotype

P{lacW}chb^S068607

(Inoue et al., 2000)

chb^S068607

(Inoue et al., 2000)

Caused by aberration

Df(3L)chb²

Cytology

Description

Imprecise excision of the P{lacW} element, resulting in a deletion extending from within the asparagine-synthetase gene to within the P{lacW} element.

(Inoue et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion

(Stramer et al., 2010)

abnormal mitotic cell cycle | recessive

(Inoue et al., 2000)

abnormal neuroanatomy

(Lee et al., 2004)

abnormal neuroanatomy (with chb⁴)

(Lee et al., 2004)

abnormal size | larval stage (with chb³)

(Inoue et al., 2000)

abnormal size | larval stage (with chb⁴)

(Inoue et al., 2000)

abnormal size | larval stage (with Df(3L)Pc-9a)

(Inoue et al., 2000)

decreased size | embryonic stage (with Df(3L)BSC553)

(Hahn et al., 2021)

Phenotype Manifest In

axon

(Beaven et al., 2015)

axon (with Df(3L)BSC553)

(Beaven et al., 2015)

axon | embryonic stage (with Df(3L)BSC553)

(Hahn et al., 2021)

embryonic/larval central nervous system | larval stage (with chb³)

(Inoue et al., 2000)

embryonic/larval central nervous system | larval stage (with chb⁴)

(Inoue et al., 2000)

embryonic/larval central nervous system | larval stage (with Df(3L)Pc-9a)

(Inoue et al., 2000)

embryonic/larval hemocyte

(Stramer et al., 2010)

microtubule

(Stramer et al., 2010)

microtubule plus-end | embryonic stage (with Df(3L)BSC553)

(Hahn et al., 2021)

mitotic cell cycle

(Inoue et al., 2000)

mitotic metaphase

(Inoue et al., 2000)

presumptive embryonic/larval central nervous system

(Lee et al., 2004)

presumptive embryonic/larval central nervous system (with chb⁴)

(Lee et al., 2004)

wing disc | larval stage (with chb³)

(Inoue et al., 2000)

wing disc | larval stage (with chb⁴)

(Inoue et al., 2000)

wing disc | larval stage (with Df(3L)Pc-9a)

(Inoue et al., 2000)

Detailed Description

Statement

Reference

Cultured primary neurons derived from either homozygous chb² or chb²/Df(3L)BSC553 embryos have a significantly reduced axon length compared to wild-type controls.

(Beaven et al., 2015)

chb² mutant hemocytes exhibit a delay in migration along the ventral midline. Individual hemocytes fail to distribute evenly at stage 15, indicating a defect in contact repulsion. chb² hemocytes are frequently seen in contact with one another for more than 15 minutes (wild-type hemocytes are rarely seen in contact for more than 10 minutes). chb² hemocytes are still capable of migration to both epithelial wounds and in response to developmental guidance cues. chb² mutant hemocytes migrating to wounds exhibit reduced directional persistence and an increase in migration speed when compared to wild-type cells.

Microtubules in chb² mutant cells are highly dynamic and are able to both polymerize and undergo catastrophe. However, unlike in wild-type cells, microtubule polymerization is unpolarized: no microtubules are stabilized or bundled, with no microtubule arm developing.

(Stramer et al., 2010)

Homozygous and chb²/chb⁴ embryos show ectopic crossing of the midline by axons in the central nervous system. Sema-2b-expressing neurons show erratic trajectories both approaching the midline and along the longitudinal neuropil in the central nervous system of homozygous embryos. The Sema-2b-positive axon tracts show a significant inward shift in their lateral position.

(Lee et al., 2004)

Mitotic defects are seen in the cells of the larval central nervous system in chb²/chb^S068607 flies. Polyploid metaphase figures (16%), diploid metaphase figures with hypercondensed chromosomes (40%) and circular metaphase figures (3%) are seen. The metaphase to anaphase ratio and the mitotic index is increased compared to wild type. Third instar chb²/Df(3L)Pc-9a, chb³/chb² or chb⁴/chb² larvae lack imaginal discs and have a small central nervous system. More than 80% of total metaphase figures are polyploid, and more than 30% of polyploid figures have greater than an 8N chromosome complement. Circular metaphase figures and anaphase figures cannot be detected.

(Inoue et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

chb² has axon phenotype, non-suppressible by CLIP-190^KO/CLIP-190^KO

(Beaven et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in axon length seen in cultured primary neurons derived from homozygous chb² embryos is unchanged if the embryos are also homozygous for CLIP-190^KO.

(Beaven et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

chb² is rescued by chb^UAS.GFP/Scer\GAL4^srp.Hemo

(Stramer et al., 2010)

chb⁴/chb² is rescued by chb^UAS.GFP/Scer\GAL4^elav.PLu

(Lee et al., 2004)

Comments

Expression of chb^{Scer\UAS.T:Avic\GFP} in hemocytes under the control of Scer\GAL4^srp.Hemo rescues microtubule arm formation in chb² mutants. In addition, expression of chb^{Scer\UAS.T:Avic\GFP} restores contact repulsion between hemocytes resulting in wild-type levels of contact time between cells.

(Stramer et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

chb²

clasp²

(Hahn et al., 2021)

dCLASP²

(Beaven et al., 2015)

orbit²

(Lee et al., 2004, Inoue et al., 2000)

Name Synonyms

orbit2

(Stramer et al., 2010)

Secondary FlyBase IDs

References (5)

Report Sections

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