FlyBase Allele Report: Dmel\CLIP-190[KO]

General Information

Symbol

Dmel\CLIP-190^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0291053

Feature type

allele

Associated gene

Dmel\CLIP-190

Associated Insertion(s)

TI{TI}CLIP-190^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Beaven et al., 2015)

Mutagen

ends-out gene targeting

(Dix et al., 2013)

Progenitor genotype

Associated Insertion(s)

TI{TI}CLIP-190^KO

Cytology

Description

Part of the CLIP-190 gene has been replaced by a loxP cassette containing w^+mW.hs.

(Beaven et al., 2015)

Deletion that removes the majority of the CLIP-190 gene, including the CAP-Gly domain (nucleotides 2L:17389389..17406580 , release 5 genome, have been removed).

(Dix et al., 2013)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:17,389,389..17,406,580 [+]

Comment:

A deletion generated by homologous recombination of the region 2L:17389389..17406580 (R5 coordinates).

(Dix et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Beaven et al., 2015)

viable

(Beaven et al., 2015, Dix et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

The length of the motor nerves is normal in stage 16 CLIP-190^KO/Df(2L)CLIP-190 embryos (also carrying Rpb11^Ubi.PB to rescue the loss of Rpb11 function in Df(2L)CLIP-190). There are no obvious changes in general morphology or overall terminal length at the neuromuscular junction of late third instar larvae.

Cultured primary neurons derived from homozygous CLIP-190^KO embryos have a normal axon length compared to wild-type controls.

(Beaven et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

CLIP-190^KO/CLIP-190^KO is a non-suppressor of axon phenotype of chb²

(Beaven et al., 2015)

CLIP-190^KO/CLIP-190^KO is a non-suppressor of axon phenotype of Apc2^g10, Apc^Q8

(Beaven et al., 2015)

CLIP-190^KO/CLIP-190^KO is a non-suppressor of axon phenotype of shot³

(Beaven et al., 2015)

CLIP-190^KO/CLIP-190^KO is a non-suppressor of axon phenotype of DCTN1-p150^Δ22

(Beaven et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in axon length seen in cultured primary neurons derived from either homozygous chb² or shot³ embryos is unchanged if the embryos are also homozygous for CLIP-190^KO.

The increase in axon length seen in cultured primary neurons derived from homozygous DCTN1-p150^Δ22 embryos is unchanged if the embryos are also homozygous for CLIP-190^KO.

Cultured primary neurons derived from doubly homozygous Apc2^g10/Apc2^g10 ; Apc^Q8/Apc^Q8 embryos have a significantly increased axon length compared to wild-type controls. This phenotype is unaffected if the embryos are also homozygous for CLIP-190^KO.

(Beaven et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93997

w^*; TI{TI}CLIP-190^KO/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CLIP-190^KO

(Bu et al., 2022, Beaven et al., 2015)

clip190^KO

(Dix et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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