FB2024_03 , released June 25, 2024
Allele: Dmel\DCTN1-p150Δ22
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General Information
Symbol
Dmel\DCTN1-p150Δ22
Species
D. melanogaster
Name
FlyBase ID
FBal0217579
Feature type
allele
Associated gene
Dmel\DCTN1-p150
Associated Insertion(s)
Carried in Construct
Also Known As
GlΔ22
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Siller et al., 2005)
Progenitor genotype
DCTN1-p150KG07739
(Siller et al., 2005)
Caused by aberration
Df(3L)GlΔ22
Cytology
Description

Imprecise excision of the insertion in GlKG07739, resulting in a deletion of approximately 3.2kb that part of the Gl transcription unit (including the presumptive transcription start site) and part of the CG8833 transcription unit.

(Siller et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Cultured primary neurons derived from homozygous embryos have a significantly increased axon length compared to wild-type controls.

      (Beaven et al., 2015)

      GlG38S/GlΔ22 larvae show normal locomotion.

      GlG38S/GlΔ22 third instar larvae have a normal number of synaptic boutons per neuromuscular junction (NMJ) in proximal abdominal segments (A2 and A3) but show a small but significant increase in the number of synaptic boutons per NMJ in distal segments (A5 and A6). The terminal boutons (the distal-most synaptic boutons) are swollen at the mutant NMJ and show an approximately 2-fold increase in bouton volume in distal segments.

      GlG38S/GlΔ22 larvae show a significant reduction in the amplitude of the evoked junctional potential (EJP) at the NMJ compared to controls.

      (Lloyd et al., 2012)

      GlΔ22/Gll1 and homozygous neuroblasts show centrosome and spindle formation defects. Defects include metaphase neuroblasts with curved spindles, unfocused spindle poles, spindles with one or two unattached centrosomes, spindles in which both centrosomes are attached to the same half-spindle, or occasionally neuroblasts with three or four centrosomes forming bipolar or multipolar spindles.

      The mitotic index and metaphase:anaphase ratio are increased in homozygous neuroblasts compared to wild type.

      (Siller et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      DCTN1-p150G38S/DCTN1-p150Δ22 has phenotype, enhanceable by Khc[+]/Khc8

      (Lloyd et al., 2012)
      NOT suppressed by
      Statement
      Reference

      DCTN1-p150Δ22 has axon phenotype, non-suppressible by CLIP-190KO/CLIP-190KO

      (Beaven et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increase in axon length seen in cultured primary neurons derived from homozygous DCTN1-p150Δ22 embryos is unchanged if the embryos are also homozygous for CLIP-190KO.

      (Beaven et al., 2015)

      GlG38S/GlΔ22 ; Khc8/+ animals rarely survive to pupal stages.

      (Lloyd et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      DCTN1-p150Δ22/DCTN1-p150l1 is rescued by DCTN1-p150CH322-82J07

      (Lloyd et al., 2012)
      Partially rescued by

      DCTN1-p150Δ22/DCTN1-p150l1 is partially rescued by DCTN1-p150UAS.cLa/Scer\GAL4T80

      (Lloyd et al., 2012)
      Comments

      Expression of GlScer\UAS.cLa under the control of Scer\GAL4T80 rescues the lethality of Gll1/GlΔ22 animals, although the rescued animals are sterile.

      Expression of GlCH322-82J07 fully rescues Gll1/GlΔ22 animals (the rescued animals are viable and fertile).

      (Lloyd et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DCTN1-p150Δ22
      GlΔ22
      (Beaven et al., 2015, Lloyd et al., 2012, Siller et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)