FB2024_03 , released June 25, 2024
Allele: Dmel\DCTN1-p150l1
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General Information
Symbol
Dmel\DCTN1-p150l1
Species
D. melanogaster
Name
FlyBase ID
FBal0005066
Feature type
allele
Associated gene
Dmel\DCTN1-p150
Associated Insertion(s)
Carried in Construct
Also Known As
Gl1-3
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Kankel and Lipshitz, 1981)
Progenitor genotype
Cytology
Description

Nucleotide substitution: C?T.

(Siller et al., 2005)

Amino acid replacement: Q932term.

(Siller et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:13,933,189..13,933,189 [+]
Nucleotide change:

C13933189T

Amino acid change:

Q932term | DCTN1-p150-PA

Reported amino acid change:

Q932term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Siller et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      GlΔ22/Gll1 neuroblasts show centrosome and spindle formation defects. Defects include metaphase neuroblasts with curved spindles, unfocused spindle poles, spindles with one or two unattached centrosomes, spindles in which both centrosomes are attached to the same half-spindle, or occasionally neuroblasts with three or four centrosomes forming bipolar or multipolar spindles.

      The mitotic index and metaphase:anaphase ratio are increased in Gll1/Df(3L)fz-GF3b neuroblasts compared to wild type.

      Tension between sister centromeres is reduced in Gll1 metaphase neuroblasts compared to wild type.

      (Siller et al., 2005)

      Gll1/Df(3L)Gl2 animals die as embryos or early first instar larvae. Cell lethal in the eye or cuticle.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Gll1 shows little or no dominant interaction with S1 in the eye.

      (Iyadurai et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      DCTN1-p150Δ22/DCTN1-p150l1 is rescued by DCTN1-p150CH322-82J07

      (Lloyd et al., 2012)

      DCTN1-p150G38S/DCTN1-p150l1 is rescued by DCTN1-p150CH322-82J07

      (Lloyd et al., 2012)
      Partially rescued by

      DCTN1-p150Δ22/DCTN1-p150l1 is partially rescued by DCTN1-p150UAS.cLa/Scer\GAL4T80

      (Lloyd et al., 2012)

      DCTN1-p150G38S/DCTN1-p150l1 is partially rescued by DCTN1-p150UAS.cLa/Scer\GAL4T80

      (Lloyd et al., 2012)
      Comments

      Expression of GlScer\UAS.cLa under the control of Scer\GAL4T80 rescues the lethality of Gll1/GlΔ22 and Gll1/GlG38S animals, although the rescued animals are sterile.

      Expression of GlCH322-82J07 fully rescues Gll1/GlΔ22 and Gll1/GlG38S animals (the rescued animals are viable and fertile).

      (Lloyd et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      DCTN1-p150l1
      Gl1-3
      (Lloyd et al., 2012, Iyadurai et al., 2008, Siller et al., 2005)
      Gll1
      l(3)Gl1-3
      (Kankel and Lipshitz, 1981)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)