FlyBase Allele Report: Dmel\msps[d03376]

General Information

Symbol

Dmel\msps^d03376

Species

D. melanogaster

Name

FlyBase ID

FBal0178804

Feature type

allele

Associated gene

Dmel\msps

Associated Insertion(s)

P{XP}msps^d03376

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

P{XP}msps^d03376

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{XP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:16,051,117..16,051,117 [+]

(Lowery et al., 2010, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Lowery et al., 2010)

abnormal size | heat sensitive, with Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Phenotype Manifest In

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^insc-Mz1407

(Lowery et al., 2010)

larval MP1 neuron, with Scer\GAL4^elav.PLu

(Lowery et al., 2010)

lateral longitudinal fascicle, with Scer\GAL4^elav.PLu

(Lowery et al., 2010)

retina | heat sensitive, with Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Detailed Description

Statement

Reference

Expression of msps^d03376 under the control of Scer\GAL4^GMR.PF does not affect eye development at 25[o]C, but when the temperature is increased to 29.5[o]C, increasing the expression level of msps^d03376, the retinal field is reduced.

Neuronal expression of msps^d03376 under the control of Scer\GAL4^elav.PLu leads to various lateral longitudinal axonal defects at low penetrance. Not only do axons follow ectopic axonal pathways, but there is also a low penetrance of ectopic midline crossing by the MP1 axon pathway. In addition, the third fascicle exhibits some missing segments. Some embryos also display mildly abnormal wavy fascicles.

Overexpression of msps^d03376 under the control of Scer\GAL4¹⁴⁰⁷ results in ISNb axons sometimes failing to reach their final muscle target. However, these mutants do not exhibit a 'bypass' phenotype, where the ISNb axon follows the ISN pathway.

(Lowery et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, msps^d03376 has abnormal size | heat sensitive phenotype, suppressible by Abl^UAS.cFa, Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/msps^d03376 is an enhancer of abnormal neuroanatomy phenotype of chb^P4

(Lowery et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/msps^d03376 is a suppressor of abnormal neuroanatomy phenotype of Abl^UAS.cFa, Scer\GAL4^insc-Mz1407

(Lowery et al., 2010)

msps^d03376, Scer\GAL4^GMR.PF is a suppressor of abnormal size | heat sensitive phenotype of Abl^UAS.cFa, Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, msps^d03376 has retina | heat sensitive phenotype, suppressible by Abl^UAS.cFa, Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/msps^d03376 is an enhancer of fascicle phenotype of chb^P4

(Lowery et al., 2010)

Suppressor of

Statement

Reference

msps^d03376 is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, chb^UAS.GFP

(Lowery et al., 2010)

msps^d03376/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, chb^UAS.GFP

(Lowery et al., 2010)

Scer\GAL4^insc-Mz1407/msps^d03376 is a suppressor of larval intersegmental nerve branch ISNb of A1-7 phenotype of Abl^UAS.cFa, Scer\GAL4^insc-Mz1407

(Lowery et al., 2010)

msps^d03376, Scer\GAL4^GMR.PF is a suppressor of eye | heat sensitive phenotype of Abl^UAS.cFa, Scer\GAL4^GMR.PF

(Lowery et al., 2010)

msps^d03376, Scer\GAL4^GMR.PF is a suppressor of retina | heat sensitive phenotype of Abl^UAS.cFa, Scer\GAL4^GMR.PF

(Lowery et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of msps^d03376 with chb^{Scer\UAS.T:Avic\GFP}, both under the control of Scer\GAL4^GMR.PF, leads to a strong suppression of the chb^{Scer\UAS.T:Avic\GFP} rough eye phenotype.

Co-expression of msps^d03376 and Abl^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PF neutralizes the opposing effects on retinal size, resulting in an eye that is nearly normal in size, albeit still abnormal in lens pattern.

Co-expression of msps^d03376 under the control of Scer\GAL4¹⁴⁰⁷ suppressed the ISNb bypass phenotype seen upon expression of Abl^Scer\UAS.cFa.

(Lowery et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

msps^d03376

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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