FlyBase Insertion Report: Dmel\P{PZ}RhoGEF2[04291]

General Information

Symbol

Dmel\P{PZ}RhoGEF2⁰⁴²⁹¹

Species

D. melanogaster

Name

FlyBase ID

FBti0009121

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)04291

Affected gene(s)

Ecol\lacZ, RhoGEF2

Viability / fertility

Associated allele(s)

Ecol\lacZ^{RhoGEF2-04291}, RhoGEF2⁰⁴²⁹¹

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 53E10 )

Sequence Location

2R:17,028,424..17,028,424 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:17,028,424..17,028,424 [-]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

53E10 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025625

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

RhoGEF2

(Sun et al., 2011, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^{RhoGEF2-04291}

Phenotypic Class Phenotype Manifest in Detailed Description

RhoGEF2⁰⁴²⁹¹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval hindgut

(Meister and Braun, 1995.10)

adult stage

lamina

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In early embryo, expressed in anterior stripe.

(Jackle and Janning, 1998.8.10)

Marker for

Reflects expression of

RhoGEF2

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)04291

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11369

cn¹ P{PZ}RhoGEF2⁰⁴²⁹¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2R:12543286-12543287 determined by FlyBase alignment of dbGSS accession AQ025625 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P1369

(Meister and Braun, 1995.10)

PZ 04291

(Sotillos and Campuzano, 2000)

P{PZ}RhoGEF2⁰⁴²⁹¹

(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}l(2)04291⁰⁴²⁹¹

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)04291⁰⁴²⁹¹

(FlyBase, 1992-)

RhoGEF2-lacZ

(Córdoba and Estella, 2014)

RhoGef2-Z

(Córdoba and Estella, 2014)

Name Synonyms

Secondary FlyBase IDs

FBti0000348
FBti0005263

References (32)

Report Sections

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