Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2R)BSC331
Date: 	Mon, 29 Oct 2007  12:07:17  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2R)BSC331
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC331 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d02420 and PBac{WH}f07747. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d02420/PBac{WH}f07747 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC331 from the segment of P{XP}d02420 
to the left of its FRT site and the segment of PBac{WH}f07747 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(2R)BSC331 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
53D14;54A1. It failed to complement RhoGEF2[04291] and Df(2R)ED2751. 
Df(2R)FDD-0035557 is a synonym for Df(2R)BSC331.