FlyBase Allele Report: Dmel\RhoGEF2[1.1]

General Information

Symbol

Dmel\RhoGEF2^1.1

Species

D. melanogaster

Name

FlyBase ID

FBal0085934

Feature type

allele

Associated gene

Dmel\RhoGEF2

Associated Insertion(s)

Carried in Construct

Also Known As

DRhoGEF2^1.1

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Nikolaidou, 2004)

Mutagen

ethyl methanesulfonate

(Barrett et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q872term.

(Nikolaidou, 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:17,037,153..17,037,153 [+]

Nucleotide change:

C17037153T

Amino acid change:

Reported amino acid change:

Q872term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Nikolaidou, 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape

(Dawes-Hoang et al., 2005)

lethal (with RhoGEF2^4.1)

(Nikolaidou and Barrett, 2004)

lethal | recessive

(Barrett et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

RhoGEF2^1.1 embryos display defects in cellularisation including an irregular, wavy cellularisation front. Despite this RhoGEF2^1.1 embryos that reach the end of cellularisation look normal. The irregularity of the cellularisation front recovers, particularly in the ventral cells, and both the increased cell depth and basal loss of myosin occur normally in these cells.

(Dawes-Hoang et al., 2005)

In mid-cellularisation embryos derived from RhoGEF2^1.1 germline clones, the furrow canals are considerably enlarged compared to wild-type and are filled with large cytoplasmic blebs. Interruptions in the regular F-actin array (a hexagonal array is normally evident in surface views) are seen in these embryos and a variable proportion of the forming cells contain multiple nuclei. Embryos derived from RhoGEF2^1.1 germline clones show normal membrane invagination and nuclear extension during cellularisation.

(Großhans et al., 2005)

RhoGEF2^1.1/RhoGEF2^4.1 animals show 97% embryonic viability, 96% first larval instar viability and 0% adult viability. Large homozygous clones in the wing disc result in defects in folding of the disc; bifurcations of the folds are found at the boundary between mutant and wild-type tissue.

(Nikolaidou and Barrett, 2004)

Homozygous clones contain on average the same number of cells as wild-type twin clones. The planar polarity of bristles in homozygous clones in the wing is normal.

(Nikolaidou, 2004)

The mitotic pattern in early embryos derived from females containing RhoGEF2^1.1 germline clones shows the same delay in the ventral domain as in wild-type embryos.

(Großhans and Wieschaus, 2000)

Embryos derived from homozygous female germline clones have normal dorso-ventral and anterior-posterior patterning, and normal mesoderm specification. Germband extension and posterior midgut invagination appear defective. The cells of the mesectoderm fail to intercalate at the ventral midline. Gastrulation is highly disorganised and ventral furrow formation never occurs. Inappropriate lateral folds are formed. Invagination of the anterior midgut is also defective.

(Barrett et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

RhoGEF2^1.1/RhoGEF2[+] is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

RhoGEF2^1.1 is a suppressor of visible phenotype of Rho1^GMR.PH

(Barrett et al., 1997)

Other

Statement

Reference

Rho1^E3.10, RhoGEF2^1.1/RhoGEF2[+] has visible phenotype

(Bayer et al., 2003)

RhoGEF2^1.1, Sb^63b/Sb[+] has visible phenotype

(Bayer et al., 2003)

RhoGEF2^1.1, Sb[+]/Sb⁷⁰ has visible phenotype

(Bayer et al., 2003)

RhoGEF2^1.1, Sb^sbd-1/Sb^sbd-201 has visible phenotype

(Bayer et al., 2003)

RhoGEF2^1.1/RhoGEF2[+], Sb^63b has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^J3.8, RhoGEF2^1.1/RhoGEF2[+] has visible phenotype

(Bayer et al., 2003)

RhoGEF2^1.1/RhoGEF2[+], Sb⁷⁰ has visible | dominant phenotype

(Bayer et al., 2003)

RhoGEF2^1.1/RhoGEF2[+], zip^Ebr has visible phenotype

(Bayer et al., 2003)

RhoGEF2^1.1/RhoGEF2[+], Sb^sbd-1/Sb^sbd-201 has visible phenotype

(Bayer et al., 2003)

Rho1^E3.10/Rho1[+], RhoGEF2^1.1 has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^J3.8/Rho1[+], RhoGEF2^1.1 has visible | dominant phenotype

(Bayer et al., 2003)

RhoGEF2^1.1, zip[+]/zip^Ebr has visible | dominant phenotype

(Bayer et al., 2003, Halsell et al., 2000)

RhoGEF2^1.1, zip^Ebr has visible | dominant phenotype

(Halsell et al., 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

RhoGEF2^1.1/RhoGEF2[+] is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

RhoGEF2^1.1 is a suppressor of eye phenotype of Rho1^GMR.PH

(Barrett et al., 1997)

RhoGEF2^1.1 is a suppressor of embryonic head | dorsal phenotype of fog^hkb.PB

(Barrett et al., 1997)

Other

Statement

Reference

Rho1^E3.10, RhoGEF2^1.1 has leg phenotype

(Bayer et al., 2003)

RhoGEF2^1.1, Sb^sbd-1/Sb^sbd-201 has leg phenotype

(Bayer et al., 2003)

RhoGEF2^1.1, Sb^63b has leg phenotype

(Bayer et al., 2003)

RhoGEF2^1.1, Sb⁷⁰ has leg phenotype

(Bayer et al., 2003)

RhoGEF2^1.1/RhoGEF2[+], Sb^63b has leg phenotype

(Bayer et al., 2003)

RhoGEF2^1.1/RhoGEF2[+], Sb⁷⁰ has leg phenotype

(Bayer et al., 2003)

RhoGEF2^1.1/RhoGEF2[+], Sb^sbd-1/Sb^sbd-201 has leg phenotype

(Bayer et al., 2003)

Rho1^J3.8, RhoGEF2^1.1 has leg phenotype

(Bayer et al., 2003)

Rho1^E3.10/Rho1[+], RhoGEF2^1.1 has leg phenotype

(Bayer et al., 2003)

Rho1^J3.8/Rho1[+], RhoGEF2^1.1 has leg phenotype

(Bayer et al., 2003)

RhoGEF2^1.1, zip[+]/zip^Ebr has leg phenotype

(Bayer et al., 2003, Halsell et al., 2000)

RhoGEF2^1.1, zip^Ebr has leg phenotype

(Bayer et al., 2003, Halsell et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

RhoGEF2^1.1 shows a weak interaction (5-24% of double heterozygotes have at least one malformed leg) with the following mutations: Sb^63b, Sb⁷⁰. br¹ fails to show a significant genetic interaction (assayed in terms of a malformed leg phenotype) with RhoGEF2^1.1. Sb^sbd-201/Sb^sbd-1 shows a weak interaction (5-24% of double mutants have at least one malformed leg) with the following mutations: RhoGEF2^1.1/+.

(Bayer et al., 2003)

33% of RhoGEF2^1.1/zip^Ebr double heterozygotes have a malformed leg phenotype.

(Halsell et al., 2000)

Dominantly suppresses the Rho1^GMR.PH rough eye phenotype. The transient depression in the dorsal head region seen in embryos carrying fog^hkb.PB in a wild-type background is not seen in embryos carrying fog^hkb.PB which are derived from homozygous RhoGEF2^1.1 female germline clones.

(Barrett et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RhoGEF2²⁰¹

(Großhans et al., 2005)

RhoGEF2³⁵⁰

(Großhans et al., 2005)

RhoGEF2^11-3

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹

(Barrett et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9121

y¹ w^*; RhoGEF2^1.1/CyO; P{neoFRT}82B ssh^1-11/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

Comments

Isolated as a dominant suppressor of the Rho1^GMR.PH rough eye phenotype.

(Barrett et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

1.1

(Nikolaidou, 2004)

DRhoGEF2^1.1

(Nikolaidou and Barrett, 2004, Bayer et al., 2003, Großhans and Wieschaus, 2000, Barrett et al., 1997)

RhoGEF2^1.1

(Denk-Lobnig et al., 2021)

RhoGEF^1.1

(Ng and Luo, 2004)

rhogef2^1.1

(Mason et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (11)

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