FlyBase Allele Report: Dmel\sd[ETX4]

General Information

Symbol

Dmel\sd^ETX4

Species

D. melanogaster

Name

FlyBase ID

FBal0032671

Feature type

allele

Associated gene

Dmel\sd

Associated Insertion(s)

P{lArB}sd^ETX4

Carried in Construct

Also Known As

sd-lacZ, sd^P(ry+,ETX4), sd-LacZ^ETX4

Key Links

Allele class

hypomorphic allele - genetic evidence

loss of function allele

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Srivastava et al., 2002, Shyamala and Chopra, 1999, Paumard-Rigal et al., 1998)

loss of function allele

(Deshpande et al., 1997)

Mutagen

Delta2-3 transposase

(Anand et al., 1990)

P-element activity

(Inamdar et al., 1993, Williams et al., 1993, Campbell et al., 1992)

Progenitor genotype

Associated Insertion(s)

P{lArB}sd^ETX4

Cytology

Description

Insertion within the first intron.

(Deshpande et al., 1997)

Insertion of a P{lacZ} element in the first intron, approximately 400bp upstream of the translation start site.

(Inamdar et al., 1993)

P-element insertion within the first intron.

(Campbell et al., 1992)

Allele components

Component

Use(s)

Inserted element

P{lArB}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | recessive

(Inamdar et al., 1993)

abnormal taste perception | recessive

(Anand et al., 1990)

viable

(Srivastava et al., 2004)

visible

(Wu et al., 2008, Delanoue et al., 2004, Srivastava et al., 2004)

visible (with sd⁹)

(Srivastava et al., 2004)

visible (with sd¹¹)

(Srivastava et al., 2004)

visible (with sd¹²)

(Srivastava et al., 2004)

visible (with sd¹³)

(Srivastava et al., 2004)

visible (with sd^58d)

(Srivastava et al., 2004)

visible (with sd^SG29.1)

(Shyamala and Chopra, 1999)

visible | recessive

(Shyamala and Chopra, 1999, Paumard-Rigal et al., 1998, Deshpande et al., 1997, Inamdar et al., 1993)

Phenotype Manifest In

capitellum

(Inamdar et al., 1993)

wing

(Wu et al., 2008, Chow et al., 2004, Srivastava et al., 2004, Bantignies et al., 2003, Srivastava and Bell, 2003, Srivastava et al., 2002, Shyamala and Chopra, 1999, Paumard-Rigal et al., 1998, Deshpande et al., 1997, Inamdar et al., 1993, Anand et al., 1990)

wing, with Scer\GAL4^vg.boundary, sd^Δ88-123.UAS

(Chow et al., 2004)

wing, with Scer\GAL4^vg.boundary, sd^Δ88-159.UAS

(Chow et al., 2004)

wing, with Scer\GAL4^vg.boundary, sd^{Δ124-159.UAS}

(Chow et al., 2004)

wing, with Scer\GAL4^vg.boundary, sd^{Δ160-219.UAS}

(Chow et al., 2004)

wing, with Scer\GAL4^vg.boundary, sd^Δ200.UAS

(Chow et al., 2004)

wing (with sd⁹)

(Srivastava et al., 2004)

wing (with sd¹¹)

(Srivastava et al., 2004)

wing (with sd¹²)

(Srivastava et al., 2004)

wing (with sd¹³)

(Srivastava et al., 2004)

wing (with sd^58d)

(Srivastava et al., 2004)

wing (with sd^SG29.1)

(Shyamala and Chopra, 1999)

wing | heat sensitive, with Scer\GAL4^vg.boundary, sd^UAS.cSa

(Chow et al., 2004)

wing | heat sensitive, with Scer\GAL4^vg.boundary, sd^Δ87.UAS

(Chow et al., 2004)

wing margin

(Marygold et al., 2011, Delanoue et al., 2004, Srivastava et al., 2004)

wing margin (with sd⁹)

(Srivastava et al., 2004)

wing margin (with sd¹¹)

(Srivastava et al., 2004)

wing margin (with sd¹²)

(Srivastava et al., 2004)

wing margin (with sd¹³)

(Srivastava et al., 2004)

wing sensillum

(Inamdar et al., 1993)

Detailed Description

Statement

Reference

sd^ETX4 hemizygous males have notched wings.

(Marygold et al., 2011)

Hemizygous males have a notched wing phenotype.

(Wu et al., 2008)

Expression of one of sd^{Δ200.Scer\UAS}, sd^{Δ124-159.Scer\UAS}, sd^{Δ88-159.Scer\UAS}, sd^{Δ160-219.Scer\UAS} and sd^{Δ88-123.Scer\UAS} when driven by Scer\GAL4^vg.boundary, lead to an enhancement of the wing phenotype seen in sd^ETX4 mutants. Expression of either sd^{Δ87.Scer\UAS} or sd^Scer\UAS.cSa driven by Scer\GAL4^vg.boundary can either rescue the sd^ETX4 defective wing phenotype, or can actually make this phenotype more severe, depending on the insertion lines used. The effect of these insertions is also temperature sensitive, with certain insertions suppressing the sd^ETX4 phenotype at one temperature and enhancing it at another.

(Chow et al., 2004)

sd^ETX4/Y males display wings with notches at the margin.

(Delanoue et al., 2004)

Mutants have notches in the wing margin. sd¹¹/sd^ETX4, sd⁹/sd^ETX4 and sd¹²/sd^ETX4 flies have a scalloped wing phenotype, with the loss of most of the wing blade tissue and erosion of the wing margin. sd¹³/sd^ETX4 flies have a scalloped wing phenotype, with little loss of wing blade tissue but variable erosion of the wing margin (ranging from very conspicuous to loss of only a few margin bristles). sd¹⁰/sd^ETX4 flies have wild-type wings.

(Srivastava et al., 2004)

Mutant animals have severely notched wings.

(Srivastava and Bell, 2003)

Mutant animals have notched wings.

(Srivastava et al., 2002)

Homozygotes show scalloping of the wing at the anterior margin and at the tip. sd^SG29.1/sd^ETX4 flies show slight nicking of the wing, comparable to that seen in sd^SG29.1 homozygotes.

(Shyamala and Chopra, 1999)

Homozygotes have gaps in the wing margin.

(Paumard-Rigal et al., 1998)

Homozygous females and hemizygous males show nicking of the anterior and lateral margins of the wing blade, with males having a more severe phenotype. sd^ETX4/Df(1)sd72b flies have vestigial wings and the capitellum is reduced. Approximately 85% of sd^ETX4 flies lack all wing sensilla. Homozygotes and hemizygotes show a reduced response to 1mM sucrose in a proboscis extension test when stimulated on the tarsal or labellar sensilla. Adults also show a reduced response to sucrose, and an enhanced preference and tolerance for salt in a feeding preference assay. The L1 neuron shows a normal response on stimulation of the labellar taste hairs with varying concentrations of sucrose, NaCl or KCl. Larvae show a strongly reduced avoidance of NaCl and KCl in a chemotaxis test. Adults and larvae show a normal response to benzaldehyde and iso-amyl acetate in olfactory assays.

(Inamdar et al., 1993)

Mutants show a general taste defect. Wings are scalloped.

(Anand et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sd^ETX4 has visible phenotype, enhanceable by yki[+]/yki^B5

(Wu et al., 2008)

sd^ETX4 has visible phenotype, enhanceable by Df(3R)P-10/+

(Delanoue et al., 2004)

sd^ETX4 has visible phenotype, enhanceable by vg[+]/vg^null

(Delanoue et al., 2004)

sd^ETX4 has visible phenotype, enhanceable by E2f[+]/E2f1⁹¹

(Delanoue et al., 2004)

sd^ETX4 has visible | recessive phenotype, enhanceable by vg^79d5

(Paumard-Rigal et al., 1998)

Suppressor of

Statement

Reference

sd^ETX4 is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Other

Statement

Reference

Scer\GAL4^ptc-559.1, sd^ETX4, vg^UAS.cZa has visible phenotype

(Paumard-Rigal et al., 1998)

Scer\GAL4^ptc-559.1, sd^ETX4, vg^UAS.cZa has lethal phenotype

(Paumard-Rigal et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

sd^ETX4 has wing margin phenotype, enhanceable by E-2g[+]/ebi^2g2

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by ebi^E90/ebi[+]

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by ebi[+]/ebi^CC3

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by E-2g[+]/ebi^2g1

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by ebi[+]/ebi^CCS-8

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by ebi[+]/ebi^E4

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by ebi^Δ1/ebi[+]

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by ebi[+]/ebi^W418X

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by ebi[+]/ebi^k16213

(Marygold et al., 2011)

sd^ETX4 has wing margin phenotype, enhanceable by ebi[+]/ebi^WKS-24

(Marygold et al., 2011)

sd^ETX4 has wing phenotype, enhanceable by yki[+]/yki^B5

(Wu et al., 2008)

sd^ETX4 has wing margin phenotype, enhanceable by Df(3R)P-10/+

(Delanoue et al., 2004)

sd^ETX4 has wing margin phenotype, enhanceable by vg[+]/vg^null

(Delanoue et al., 2004)

sd^ETX4 has wing margin phenotype, enhanceable by E2f[+]/E2f1⁹¹

(Delanoue et al., 2004)

sd^ETX4 has wing phenotype, enhanceable by sens^Ly-1

(Srivastava and Bell, 2003)

sd^ETX4 has wing phenotype, enhanceable by vg^79d5/vg¹

(Srivastava et al., 2002)

sd^ETX4 has wing phenotype, enhanceable by vg^79d5

(Paumard-Rigal et al., 1998)

sd^ETX4 has adult thorax & macrochaeta phenotype, enhanceable by vg^79d5

(Paumard-Rigal et al., 1998)

NOT Enhanced by

Statement

Reference

sd^ETX4 has wing margin phenotype, non-enhanceable by ebi[+]/ebi^P7

(Marygold et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^vg.boundary, sd^ETX4, sd^UAS.cSa has wing phenotype, suppressible by vg^UAS.cKa/Scer\GAL4^vg.boundary

(Chow et al., 2004)

Scer\GAL4^vg.boundary, sd^Δ200.UAS, sd^ETX4 has wing phenotype, suppressible by vg^UAS.cKa/Scer\GAL4^vg.boundary

(Chow et al., 2004)

sd^ETX4 has wing phenotype, suppressible by wg^UAS.lacZ/Scer\GAL4^vg.boundary

(Srivastava and Bell, 2003)

sd^ETX4, vg^79d5/vg¹ has wing phenotype, suppressible by sd::vg^UAS.sdTEA/Scer\GAL4^vg.PM

(Srivastava et al., 2002)

sd^ETX4 has wing phenotype, suppressible by Hsap\TEAD1^hs.PD

(Deshpande et al., 1997)

NOT suppressed by

Statement

Reference

sd^ETX4 has wing phenotype, non-suppressible by Fab-7¹

(Bantignies et al., 2003)

Enhancer of

Statement

Reference

sd^ETX4 is an enhancer of wing phenotype of sens^Ly-1

(Srivastava and Bell, 2003)

Suppressor of

Statement

Reference

sd^ETX4 is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

sd^ETX4 is a suppressor of eye phenotype of Hsap\VGLL1^UAS.cVa, Scer\GAL4^ptc-559.1

(Vaudin et al., 1999)

sd^ETX4 is a suppressor of eye phenotype of Hsap\VGLL1^UAS.cVa, Scer\GAL4^da.G32

(Vaudin et al., 1999)

Other

Statement

Reference

Scer\GAL4^ptc-559.1, sd^ETX4, vg^UAS.cZa has eye phenotype

(Paumard-Rigal et al., 1998)

Scer\GAL4^ptc-559.1, sd^ETX4, vg^UAS.cZa has adult head phenotype

(Paumard-Rigal et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for ebi^2g2, ebi^E90, ebi^CC3, ebi^2g1, ebi^CCS-8, ebi^E4, ebi^Δ1, ebi^W418X, ebi^k16213 or ebi^WKS-24 enhances the notched wing margin phenotype seen in sd^ETX4 hemizygous males.

(Marygold et al., 2011)

yki^B5/+ enhances the notched wing phenotype of sd^ETX4/Y males.

(Wu et al., 2008)

The defective wing phenotypes seen when sd^Scer\UAS.cSa or sd^{Δ200.Scer\UAS} are expressed under control of Scer\GAL4^vg.boundary in sd^ETX4 mutants is alleviated when vg^Scer\UAS.cKa is overexpressed.

(Chow et al., 2004)

sd[ETX4]/Y males display an enhanced wing phenotype in a vg^null/+ background.

sd^ETX4/Y, E2f⁹¹/+ males display a severe mutant wing phenotype compared to sd^ETX4/Y males.

sd^ETX4/Y males display an enhanced wing phenotype in Df(3R)P-10/+ background.

(Delanoue et al., 2004)

The addition of sd::vg^{Scer\UAS.sdTEA} (driven by Scer\GAL4^vg.PM) to sd^ETX4 animals, almost completely rescues the wing phenotype with only a small amount of wing notching and occasional loss of bristles on the wing margin. The L5 wing vein is also usually incompletely formed. sd^ETX4, vg¹/vg^79d5 wings are very small and deformed. The addition of sd::vg^{Scer\UAS.sdTEA} (driven by Scer\GAL4^vg.PM) significantly rescues the wing defects.

(Srivastava et al., 2002)

sd^ETX4 vg^79d5 double homozygotes show a significant reduction in wing size and an uplifting of the postscutellar bristles, similar to the phenotype of vg¹ homozygotes. Flies heterozygous for vg^79d5 in a sd^ETX4 background have a phenotype almost as strong as sd^ETX4 vg^79d5 double homozygotes. Flies heterozygous for vg^null in a sd^ETX4 background do not show such a significant reduction in the wing as flies heterozygous for vg^79d5 in a sd^ETX4 or sd¹ background. Females expressing vg^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 in a sd^ETX4/+ background die in the late pupal stage. They show ectopic wing-like outgrowths at the posterior of the head capsule, with strong deformation of the eye. Males expressing vg^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 in a hemizygous sd^ETX4 background do not show such extensive wing-like outgrowths and show only slight deformation of the eye. These males can emerge from the pupae, although they die within a few hours.

(Paumard-Rigal et al., 1998)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\TDU^Scer\UAS.cVa under the control of Scer\GAL4^ptc-559.1 or Scer\GAL4^da.G32 results in a large increase in eye size. This is partially suppressed in a sd^ETX4 background.

(Vaudin et al., 1999)

Defects in the wing blade can be rescued by constructs carrying Hsap\TCF13^hs.PD.

(Deshpande et al., 1997)

Complementation and Rescue Data

Complements

sd¹⁰

(Srivastava et al., 2004)

Partially complements

sd^SG29.1

(Shyamala and Chopra, 1999)

Fails to complement

sd¹³