FlyBase Allele Report: Dmel\sd[9]

General Information

Symbol

Dmel\sd⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0015355

Feature type

allele

Associated gene

Dmel\sd

Associated Insertion(s)

Carried in Construct

Also Known As

sd^3L

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Katzen and Bishop, 1996, Katzen, 1990)

Progenitor genotype

Cytology

Description

Amino acid replacement: L232term.

(Srivastava et al., 2004)

The coordinates given for the mutant lesion refer to the sd E21 isoform. The protein encoded by the sd E7 isoform appears to be unaffected in this mutant.

(Srivastava et al., 2004)

Nucleotide substitution: T?A.

(Srivastava et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:15,823,439..15,823,439 [+]

Nucleotide change:

T15823439A

Reported nucleotide change:

T?A

Amino acid change:

Reported amino acid change:

L232term

(Srivastava et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval cardial cell

(Deng et al., 2009)

larval segmental nerve

(Guss et al., 2013)

muscle cell of ventral oblique muscle 4

(Deng et al., 2009)

muscle cell of ventral oblique muscle 5

(Deng et al., 2009)

muscle cell of ventral oblique muscle 6

(Deng et al., 2009)

wing (with sd^ETX4)

(Srivastava et al., 2004)

wing margin (with sd^ETX4)

(Srivastava et al., 2004)

Detailed Description

Statement

Reference

Muscle structure is generally normal in sd⁹ embryos, although an altered distribution of nuclei is observed.

sd⁹ embryos exhibit a disruption in segmental nerve branch growth. The length of SNb is shorter in sd⁹ mutants than in controls, and the stereotyped branching pattern is disrupted.

(Guss et al., 2013)

The waves of contraction seen in newly hatched larvae are much slower than normal in sd⁹ hemizygotes, taking approximately 3 times as long to pass from posterior tip to anterior tip compared to wild type.

Stage 13 hemizygous embryos often have less than the normal number of cardiac cells, and many of the remaining cardiac cells have larger nuclei than normal. Somatic muscle defects are also seen, with the ventral somatic muscles (VO4-VO6) being lost or abnormal.

(Deng et al., 2009)

Homozygotes die as first instar larvae. sd⁹/sd^ETX4 flies have a scalloped wing phenotype, with the loss of most of the wing blade tissue and erosion of the wing margin.

(Srivastava et al., 2004)

Dorsal medial muscles in sd⁹ mutants display no obvious phenotype.

(Bernard et al., 2003)

Larvae exhibit slow growth. Heterozygotes with viable sd alleles exhibit an extreme wing phenotype.

(Campbell et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

rhea¹, sd⁹ has somatic muscle cell phenotype

(Deng et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

sd⁹; rhea¹ double mutants produce a mild somatic musculature phenotype, but otherwise are indistinguishable from rhea¹ mutants.

(Deng et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sd^ETX4

(Srivastava et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

em3

(Katzen and Bishop, 1996)

sd^3L

(Guss et al., 2013, Deng et al., 2010, Deng et al., 2009, Srivastava et al., 2004, Bernard et al., 2003, Campbell et al., 1992, Campbell et al., 1991)

sd⁹

sd^EM3

(Katzen, 1990)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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