FlyBase Allele Report: Dmel\sd[UAS.cSa]

General Information

Symbol

Dmel\sd^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Simmonds

FlyBase ID

FBal0094348

Feature type

allele

Associated gene

Dmel\sd

Associated Insertion(s)

Carried in Construct

P{UAS-sd.S}

Also Known As

UAS-sd

Key Links

Transgenic product class

cDNA

(Simmonds et al., 1998)

wild_type

(Simmonds et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Simmonds et al., 1998)

wild_type

(Simmonds et al., 1998)

Mutagen

in vitro construct

(Simmonds et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-sd.S}

Cytology

Description

A 2.05kb XmnI-ClaI sd cDNA fragment is expressed under the control of UASt regulatory sequences.

(Simmonds et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by yki^UAS.cHa

(Ziosi et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^GMR.PF

(Wu et al., 2008)

abnormal size, with Scer\GAL4^vg.PU

(Wu et al., 2008)

increased cell death, with Scer\GAL4^ptc-559.1

(Liu et al., 2000)

lethal, with Scer\GAL4^C587

(Li et al., 2015)

lethal - all die before end of pupal stage, with Scer\GAL4^ptc-559.1

(Garg et al., 2007)

some die during pupal stage, with Scer\GAL4^ptc-559.1

(Garg et al., 2007)

visible, with Scer\GAL4^GMR.PF

(Wu et al., 2008)

visible, with Scer\GAL4^GMR.PU

(Qing et al., 2014)

visible, with Scer\GAL4^ptc-559.1

(Simmonds et al., 1998)

visible, with Scer\GAL4^vg.PU

(Wu et al., 2008)

visible | heat sensitive, with Scer\GAL4^dpp.blk1

(Simmonds et al., 1998)

Phenotype Manifest In

adult head, with Scer\GAL4^ptc-559.1

(Simmonds et al., 1998)

eye, with Scer\GAL4^GMR.PF

(Wu et al., 2008)

eye, with Scer\GAL4^GMR.PU

(Qing et al., 2014)

eye, with Scer\GAL4^ptc-559.1

(Garg et al., 2007)

indirect flight muscle cell, with Scer\GAL4¹¹⁵¹

(Bernard et al., 2009)

lamina, with Scer\GAL4^ptc-559.1

(Garg et al., 2007)

leg, with Scer\GAL4^ptc-559.1

(Garg et al., 2007)

lobula plate, with Scer\GAL4^ptc-559.1

(Garg et al., 2007)

wing, with Scer\GAL4^vg.PU

(Wu et al., 2008)

wing | heat sensitive, with Scer\GAL4^dpp.blk1

(Simmonds et al., 1998)

wing | heat sensitive, with Scer\GAL4^vg.boundary, sd^58d

(Chow et al., 2004)

wing | heat sensitive, with Scer\GAL4^vg.boundary, sd^ETX4

(Chow et al., 2004)

wing disc | somatic clone, with Scer\GAL4^Act5C.PI

(Liu et al., 2000)

Detailed Description

Statement

Reference

Expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^C587 is lethal but when inhibited during development by keeping flies at low temperature the flies survive to adulthood and do not show any germarium defects.

(Li et al., 2015)

Expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU leads to reduced eye size.

(Qing et al., 2014)

Mosaic clones expressing sd^Scer\UAS.cSa under the control of Scer\GAL4^αTub84B.PP do not overgrow compared to wild-type controls.

(Ziosi et al., 2010)

Developing indirect flight muscles degenerate in animals expressing sd^Scer\UAS.cSa under the control of Scer\GAL4¹¹⁵¹.

(Bernard et al., 2009)

Expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF results in decreased eye size compared to controls.

Expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^vg.PU results in a reduction in wing size compared to controls.

(Wu et al., 2008)

Pharate adults expressing sd^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 have eye protrusions and truncated legs. An ectopic cluster of cells is present below the basal membrane of the ommatidia in these animals. The lobula plate and lamina are missing and the brain is physically separated from the optic lobe. The development of the ommatidia remains intact in these animals. In most pupae, the head remains inside the abdominal cavity.

(Garg et al., 2007)

Expression of sd^Scer\UAS.cSa driven by Scer\GAL4^vg.boundary can either rescue the sd^ETX4 and sd^58d defective wing phenotypes, or can actually make these phenotypes more severe, depending on the sd^Scer\UAS.cSa insertion line used. The effect of these insertions is also temperature sensitive, with certain insertions suppressing the sd^ETX4 and sd^58d phenotypes at one temperature and enhancing these phenotypes at another.

(Chow et al., 2004)

Clones of cells over-expressing sd^Scer\UAS.cSa (under the control of Scer\GAL4^Act5C.PI) fail to grow in the hinge and notal regions of the disc. Expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 induces apoptotic cell death in the notum.

(Liu et al., 2000)

Flies expressing sd^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 do not produce ectopic wing tissue in the eye. Flies expressing sd^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 at 25^oC show incomplete formation of the wing margin. At 29^oC, the phenotype is more severe and almost no wing tissue is formed. Flies expressing sd^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 show loss of head tissue.

(Simmonds et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, sd^UAS.cSa has visible phenotype, enhanceable by Ncoa6^HMS00664, Scer\GAL4^GMR.PU

(Qing et al., 2014)

Scer\GAL4^GMR.PU, sd^UAS.cSa has visible phenotype, enhanceable by trr^HMS01019/Scer\GAL4^GMR.PU

(Qing et al., 2014)

Scer\GAL4^GMR.PF, sd^UAS.cSa has abnormal size phenotype, enhanceable by yki[+]/yki^B5

(Wu et al., 2008)

Scer\GAL4^GMR.PF, sd^UAS.cSa has visible phenotype, enhanceable by yki[+]/yki^B5

(Wu et al., 2008)

Scer\GAL4^vg.PU, sd^UAS.cSa has abnormal size phenotype, enhanceable by yki[+]/yki^B5

(Wu et al., 2008)

Scer\GAL4^vg.PU, sd^UAS.cSa has visible phenotype, enhanceable by yki[+]/yki^B5

(Wu et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, sd^UAS.cSa has visible phenotype, suppressible | partially by Scer\GAL4^dpp.blk1/vg^UAS.cKa

(Simmonds et al., 1998)

Enhancer of

Statement

Reference

sd^UAS.cSa/Scer\GAL4^αTub84B.PP is an enhancer of increased cell size | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

sd^UAS.cSa/Scer\GAL4^αTub84B.PP is an enhancer of hyperplasia | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

sd^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, yki^UAS.cHa

(Wu et al., 2008)

sd^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of abnormal size phenotype of Scer\GAL4^GMR.PF, yki^UAS.cHa

(Wu et al., 2008)

Suppressor of

Statement

Reference

sd^UAS.cSa/Scer\GAL4^vg.PU is a suppressor of visible phenotype of Scer\GAL4^vg.PU, vg^ΔACT.UAS

(Garg et al., 2007)

sd^UAS.cSa/Scer\GAL4^dpp.blk1 is a suppressor | partially of visible phenotype of Scer\GAL4^dpp.blk1, vg^UAS.cKa

(Simmonds et al., 1998)

Other

Statement

Reference

Scer\GAL4^GMR.PF, sd^UAS.cSa, yki^UAS.cHa has abnormal eye color phenotype

(Wu et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, sd^UAS.cSa has eye phenotype, enhanceable by Ncoa6^HMS00664, Scer\GAL4^GMR.PU

(Qing et al., 2014)

Scer\GAL4^GMR.PU, sd^UAS.cSa has eye phenotype, enhanceable by trr^HMS01019/Scer\GAL4^GMR.PU

(Qing et al., 2014)

Scer\GAL4^GMR.PF, sd^UAS.cSa has eye phenotype, enhanceable by yki[+]/yki^B5

(Wu et al., 2008)

Scer\GAL4^vg.PU, sd^UAS.cSa has wing phenotype, enhanceable by yki[+]/yki^B5

(Wu et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^vg.boundary, sd^58d, sd^UAS.cSa has wing phenotype, suppressible by vg^UAS.cKa/Scer\GAL4^vg.boundary

(Chow et al., 2004)

Scer\GAL4^vg.boundary, sd^ETX4, sd^UAS.cSa has wing phenotype, suppressible by vg^UAS.cKa/Scer\GAL4^vg.boundary

(Chow et al., 2004)

Scer\GAL4^dpp.blk1, sd^UAS.cSa has wing phenotype, suppressible | partially by Scer\GAL4^dpp.blk1/vg^UAS.cKa

(Simmonds et al., 1998)

Enhancer of

Statement

Reference

sd^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, yki^UAS.cHa

(Wu et al., 2008)

Suppressor of

Statement

Reference

sd^UAS.cSa/Scer\GAL4^vg.PU is a suppressor of wing phenotype of Scer\GAL4^vg.PU, vg^ΔACT.UAS

(Garg et al., 2007)

sd^UAS.cSa/Scer\GAL4^vg.PU is a suppressor of wing blade phenotype of Scer\GAL4^vg.PU, vg^ΔACT.UAS

(Garg et al., 2007)

sd^UAS.cSa/Scer\GAL4^dpp.blk1 is a suppressor | partially of eye phenotype of Scer\GAL4^dpp.blk1, vg^UAS.cKa

(Simmonds et al., 1998)

sd^UAS.cSa/Scer\GAL4^dpp.blk1 is a suppressor | partially of wing | ectopic phenotype of Scer\GAL4^dpp.blk1, vg^UAS.cKa

(Simmonds et al., 1998)

Other

Statement

Reference

Scer\GAL4^GMR.PF, sd^UAS.cSa, yki^UAS.cHa has eye phenotype

(Wu et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Ncoa6^HMS00664 enhances the reduction in eye size seen when sd^Scer\UAS.cSa is expressed under the control of Scer\GAL4^GMR.PU.

Expression of trr^HMS01019 enhances the reduction in eye size seen when sd^Scer\UAS.cSa is expressed under the control of Scer\GAL4^GMR.PU.

(Qing et al., 2014)

Mosaic clones expressing sd^Scer\UAS.cSa and yki^Scer\UAS.cHa under the control of Scer\GAL4^αTub84B.PP overgrow relative to yki^Scer\UAS.cHa clones.

(Ziosi et al., 2010)

Co-expression of sd^Scer\UAS.cSa and yki^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF results in eyes which are severely overgrown (and much larger than the eyes of animals expressing yki^Scer\UAS.cHa alone under the control of Scer\GAL4^GMR.PF) and folded. There is a general loss of eye pigmentation in the posterior region of the eye.

The reduction in eye size caused by expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF is significantly enhanced by yki^B5/+.

The reduction in wing size caused by expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^vg.PU is enhanced by yki^B5/+.

(Wu et al., 2008)

Co-expression of sd^Scer\UAS.cSa rescues the bristle and wing margin phenotypes caused by expression of vg^{ΔACT.Scer\UAS} under the control of Scer\GAL4^vg.PU.

(Garg et al., 2007)

The defective wing phenotype seen when sd^Scer\UAS.cSa is expressed under control of Scer\GAL4^vg.boundary in sd^ETX4 and sd^58d mutants is alleviated when vg^Scer\UAS.cKa is overexpressed.

(Chow et al., 2004)

Partially suppresses the massive outgrowth of wing tissue from the eye seen in flies expressing vg^Scer\UAS.cKa under the control of Scer\GAL4^dpp.blk1. The wing phenotype caused by sd^Scer\UAS.cSa expressed under the control of Scer\GAL4^dpp.blk1 is partially suppressed if the flies are also coexpressing vg^Scer\UAS.cKa.

(Simmonds et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

sd^UAS.cSa/Scer\GAL4^vg.boundary rescues sd^58d

(Magico and Bell, 2011)

Comments

Expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^vg.boundary restores wing development in sd^58d mutants.

(Magico and Bell, 2011)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9373

w^*; P{UAS-sd.S}V1

9374

w^*; P{UAS-sd.S}V3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sd^Scer\UAS.cSa

sd^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Simmonds

(Simmonds et al., 1998)

Secondary FlyBase IDs

References (18)

Report Sections

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