FlyBase Allele Report: Dmel\lz[ts1]

General Information

Symbol

Dmel\lz^ts1

Species

D. melanogaster

Name

FlyBase ID

FBal0011835

Feature type

allele

Associated gene

Dmel\lz

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

heat sensitive hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

heat sensitive hypomorphic allele - genetic evidence

(Gupta et al., 1998)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: N351I.

(Jackson Behan et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:9,288,521..9,288,521 [+]

Nucleotide change:

A9288521T

Reported nucleotide change:

A11268T

Amino acid change:

N351I | lz-PA; N351I | lz-PB

Reported amino acid change:

N351I

(Jackson Behan et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antennal sense organ | precursor

(Gupta et al., 1998)

basiconic sensillum

(Bhalerao et al., 2003)

basiconic sensillum of antennal segment 3

(Stocker et al., 1993)

basiconic sensillum of antennal segment 3 | heat sensitive

(Gupta et al., 1998, Gupta and Rodrigues, 1995)

embryonic/larval crystal cell

(Lebestky et al., 2000)

eye | heat sensitive

(Wildonger et al., 2005, Siddall et al., 2003, Kaminker et al., 2001, Batterham et al., 1996)

eye | posterior | heat sensitive

(Behan et al., 2002)

eye disc | heat sensitive

(Siddall et al., 2003)

female accessory gland | temperature conditional

(Chatterjee et al., 2011)

interommatidial bristle | heat sensitive

(Batterham et al., 1996, Gupta and Rodrigues, 1995)

lens | heat sensitive

(Siddall et al., 2003, Batterham et al., 1996)

maxillary palp basiconic sensillum

(Gupta and Rodrigues, 1995)

ommatidium | heat sensitive

(Kaminker et al., 2001, Batterham et al., 1996, Gupta and Rodrigues, 1995)

retina | heat sensitive

(Siddall et al., 2003)

secondary pigment cell | heat sensitive

(Wildonger et al., 2005)

spermatheca | temperature conditional

(Chatterjee et al., 2011)

tertiary pigment cell | heat sensitive

(Wildonger et al., 2005)

third segment of antenna

(Stocker et al., 1993)

trichoid sensillum of antennal segment 3

(Stocker et al., 1993)

trichoid sensillum of antennal segment 3 | ectopic | heat sensitive

(Gupta et al., 1998)

trichoid sensillum of antennal segment 3 | heat sensitive

(Gupta and Rodrigues, 1995)

Detailed Description

Statement

Reference

lz^ts1 mutant females raised at 16[o]C and shifted to 29[o]C at third instar larval stage fail to form spermathecae and accessory glands. Mutants shifted to 29[o]C at 24 hours after pupal do show formation of spermathecal ducts, but at least one of the spermathecal caps is absent in these animals.

(Chatterjee et al., 2011)

lz^ts1 pupal retina that have been temperature-shifted (to the non-permissive temperature of 33^oC at 20 hours after pupal formation) exhibit an increase in the number of 2^o and 3^o pigment cells compared to lz^ts1 flies at the permissive temperature (25^oC) or controls (at 49 hours APF). On average, there are an additional two cells per ommatidium, which is similar to the number of cells that normally die. At 55 hours after pupal formation retina still exhibit additional 2^o and 3^o pigment cells, indicating that apoptosis is not delayed. There is a reduction in apoptosis in lz^ts1 retina, although perimeter cell death is not affected in lz^ts1 mutants. Temperature-shifted lz^ts1 adult eyes are only mildly rough and have wild-type pigmentation.

(Wildonger et al., 2005)

lz^ts1 flies lack all basiconic sense organs.

(Bhalerao et al., 2003)

At 18^oC, sections through the ommatidia appear normal in lz^ts1 mutants, while at 29^oC defects in the lens and fenestrated membrane are seen. Ectopic cell death is seen in third larval instar eye discs at 29^oC.

(Siddall et al., 2003)

The eye appears normal at 25^oC in lz^ts1 mutants, while at 29^oC, posterior eye defects are seen.

(Behan et al., 2002)

Mutant flies have near wild-type eyes at 25^oC, but have a rough eye phenotype at 29^oC.

(Kaminker et al., 2001)

lz^ts1/Y flies raised at 25^oC have wild-type eyes.

(Flores et al., 2000)

Crystal cells develop normally at 25^oC but development is blocked at 29^oC.

(Lebestky et al., 2000)

The number of sense organ founder cells seen in the antennal disc 10 hours after pupariation is reduced compared to wild-type in animals pulsed at 29^oC from the second larval instar stage onwards. The number of basiconic sensilla of the antenna is reduced compared to wild-type at 25^oC. The number of trichoid sensilla and coeloconic sensilla is unaffected at this temperature. The number of trichoid sensilla is increased compared to wild-type at 28^oC. The trichoid region of the antenna is largely unaffected, but several "ectopic" trichoid sensilla appear in the region occupied exclusively by basiconic sensilla in the wild-type.

(Gupta et al., 1998)

The amplitude of the physiological response of the antenna to ethyl acetate is reduced compared to wild-type, with the severity of the phenotype increasing as the temperature increases. This phenotype is at least partially rescued if the flies are also carrying Dp(1;Y)y⁺lz⁺.

(Riesgo-Escovar et al., 1997)

Homozygotes have normal eyes when raised at 18^oC. When raised at 25^oC, subtle eye defects are seen; bristles of the hair nerve groups grow abnormally long and may be misplaced, although lenses are generally well formed. At 29^oC, flies have a more severe phenotype, with poorly formed, fused or missing lenses, abnormally long bristles that are misplaced and a general lack of ommatidial structure.

(Batterham et al., 1996)

Adults show missing, fused and reduced ommatidia and absence or duplication of several interommatidial bristles when raised at the restrictive temperature of 28^oC. The eye phenotype overlaps wild-type at 25^oC, the only defect being the incorrect location of some interommatidial bristles. The number of basiconic sensilla on the antenna is reduced by 30% at 25^oC. Flies raised at 28^oC show a striking reduction in the number of basiconic sensilla on the antenna, which is accompanied by a small but significant increase in the number of trichoid sensilla. The basiconic-like sensilla of the maxillary palps occasionally have abnormal shaft morphology, being composed of a short spine surrounded by a socket. The eye phenotype but not the antennal or maxillary palp sensilla phenotype of lz^ts1 flies is dominantly enhanced by E(lz)15¹, E(lz)53¹, E(lz)174¹, E(lz)178¹ and E(lz)205¹.

(Gupta and Rodrigues, 1995)

The antennal funiculus is reduced in size in hemizygous males raised at 28^oC or 29^oC. All large and small basiconic sensilla on the antennal funiculus are missing, and the number of trichoid sensilla on the antennal funiculus is reduced. The number of coeloconic sensilla on the antennal funiculus is normal. The density of the coeloconic sensilla on the antennal funiculus is increased and the density of the trichoid sensilla on the antennal funiculus is decreased compared to wild-type in hemizygous males raised at 29^oC. The density of the coeloconic and trichoid sensilla on the antennal funiculus is increased compared to wild-type in hemizygous males raised at 28^oC. The antennal funiculus is reduced in size in hemizygous males raised at 25^oC or 27^oC. The number of large and small basiconic sensilla on the antennal funiculus is greatly reduced, and the number of trichoid sensilla on the antennal funiculus is increased. The number of coeloconic sensilla on the antennal funiculus is normal. The density of the coeloconic and trichoid sensilla on the antennal funiculus is increased and the density of the basiconic sensilla on the antennal funiculus is decreased compared to wild-type. The antennal funiculus is reduced in size in hemizygous males raised at 18^oC. The number of large and small basiconic sensilla is reduced. The density of the coeloconic and trichoid sensilla on the antennal funiculus is increased and the density of the small basiconic sensilla on the antennal funiculus is decreased compared to wild-type.

(Stocker et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

lz^ts1 has visible phenotype, enhanceable by Ras85D^N17.hs.2sev

(Behan et al., 2002)

lz^ts1 has visible phenotype, enhanceable by aop^ACT.sev

(Behan et al., 2002)

lz^ts1 has visible phenotype, enhanceable by aop^S2382/aop[+]

(Behan et al., 2002)

lz^ts1 has visible phenotype, enhanceable by aop^ACT.GMR

(Behan et al., 2002)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by Bgb[+]/Bgb⁹

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by Bgb^D/Bgb[+]

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by Bgb^D/Df(3L)Bgb-K4

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by +/Df(3L)Bgb-K4

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by Hsp83[+]/Hsp83^3C

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by Hsp83^4C/Hsp83[+]

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by en(lz)Y[+]/en(lz)Y^Y

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by osa^4F/osa[+]

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by osa^4H/osa[+]

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by sv[+]/sv^I

(Kaminker et al., 2001)

lz^ts1 has visible | heat sensitive phenotype, enhanceable by sv[+]/sv^4G

(Kaminker et al., 2001)

lz^ts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)53¹

(Gupta and Rodrigues, 1995)

lz^ts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)174¹

(Gupta and Rodrigues, 1995)

lz^ts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)178¹

(Gupta and Rodrigues, 1995)

lz^ts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)205¹

(Gupta and Rodrigues, 1995)

lz^ts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)15¹

(Gupta and Rodrigues, 1995)

Suppressed by

Statement

Reference

lz^ts1 has visible phenotype, suppressible by Ras85D^V12.sev

(Behan et al., 2002)

Other

Statement

Reference

Egfr^tsla, lz^ts1 has increased cell death phenotype

(Wildonger et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

lz^ts1 has eye phenotype, enhanceable by Ras85D^N17.hs.2sev

(Behan et al., 2002)

lz^ts1 has eye phenotype, enhanceable by aop^ACT.sev

(Behan et al., 2002)

lz^ts1 has eye phenotype, enhanceable by aop^S2382/aop[+]

(Behan et al., 2002)

lz^ts1 has eye phenotype, enhanceable by aop^ACT.GMR

(Behan et al., 2002)

lz^ts1 has eye phenotype, enhanceable by Bgb[+]/Bgb⁹

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by Bgb^D/Bgb[+]

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by Bgb^D/Df(3L)Bgb-K4

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by +/Df(3L)Bgb-K4

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by Hsp83[+]/Hsp83^3C

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by Hsp83^4C/Hsp83[+]

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by en(lz)Y[+]/en(lz)Y^Y

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by osa^4F/osa[+]

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by osa^4H/osa[+]

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by sv[+]/sv^I

(Kaminker et al., 2001)

lz^ts1 has eye phenotype, enhanceable by sv[+]/sv^4G

(Kaminker et al., 2001)

lz^ts1 has phenotype, enhanceable by sv^spa-pol/sv[+]

(Flores et al., 2000)

lz^ts1 has eye phenotype, enhanceable by E(lz)53¹

(Gupta and Rodrigues, 1995)

lz^ts1 has eye phenotype, enhanceable by E(lz)174¹

(Gupta and Rodrigues, 1995)

lz^ts1 has eye phenotype, enhanceable by E(lz)178¹

(Gupta and Rodrigues, 1995)

lz^ts1 has eye phenotype, enhanceable by E(lz)205¹

(Gupta and Rodrigues, 1995)

lz^ts1 has eye phenotype, enhanceable by E(lz)15¹

(Gupta and Rodrigues, 1995)

Suppressed by

Statement

Reference

lz^ts1 has eye phenotype, suppressible by Ras85D^V12.sev

(Behan et al., 2002)

Suppressor of

Statement

Reference

lz^ts1/Scer\GAL4^hs.2sev is a suppressor of tertiary pigment cell phenotype of Scer\GAL4^hs.2sev, aos^UAS.cHa

(Wildonger et al., 2005)

lz^ts1/Scer\GAL4^hs.2sev is a suppressor of secondary pigment cell phenotype of Scer\GAL4^hs.2sev, aos^UAS.cHa

(Wildonger et al., 2005)

lz^ts1 is a suppressor of eye phenotype of hid^GMR.PG

(Wildonger et al., 2005)

lz^ts1 is a suppressor of eye phenotype of rpr^GMR.PW

(Wildonger et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Reducing lz activity by transferring lz^ts1 W^GMR.PG flies to the non-permissive temperature of 33^oC partially suppresses the W^GMR.PG eye phenotype. Reducing lz activity by transferring lz^ts1 rpr^GMR.PW flies to the non-permissive temperature of 33^oC partially suppresses the rpr^GMR.PW eye phenotype. Expression of argos^Scer\UAS.cHa, under the control of Scer\GAL4^hs.2sev in temperature-shifted lz^ts1 mutants results in a few small clusters of 2^o/3^o cells, which are not seen in argos^Scer\UAS.cHa Scer\GAL4^hs.2sev single mutants. Reducing Egfr signaling (using temperature-sensitive Egfr^tsla) in lz^ts1 retinas results in an increase in cell death.

(Wildonger et al., 2005)

lz^ts1 ; aop^ACT.GMR flies have a markedly reduced eye at 29^oC, with the loss of all ommatidial structures. aop^S2382/+ enhances the lz^ts1 phenotype at 29^oC, with defects extending throughout the eye. Ras85D^N17.hs.2sev enhances the lz^ts1 eye phenotype, making the eye rough at the permissive temperature for lz^ts1 and severely defective at 29^oC. Ras85D^V12.sev suppresses the posterior flattening of the eye seen in lz^ts1 flies at 29^oC.

(Behan et al., 2002)

Rare lz^ts1 ; Bgb^D/Df(3L)Bgb-K4 escapers are seen. These flies are extremely weak and can only be rescued if they are dissected from the pupal case. They have a strong adult eye phenotype that is identical to that of a null lz mutant.

(Kaminker et al., 2001)

sv^spa-pol dominantly enhances the lz^ts1 phenotype.

(Flores et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

lz^50e

(Batterham et al., 1996)

lz^77a7

(Batterham et al., 1996)