FlyBase Allele Report: Dmel\Ras85D[N17.hs.2sev]

General Information

Symbol

Dmel\Ras85D^N17.hs.2sev

Species

D. melanogaster

Name

FlyBase ID

FBal0048925

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{sevhs-Ras1^N17}

Key Links

Transgenic product class

missense_variant

(Allard et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Allard et al., 1996)

Mutagen

in vitro construct

(Allard et al., 1996)

Progenitor genotype

Carried in construct

P{sevhs-Ras1^N17}

Cytology

Description

Construct: Amino acid replacement: T17N. Mutant protein is expressed under the transcriptional control of an Hsp70 promoter and two copies of the sev enhancer.

(Allard et al., 1996)

Allele components

Component

Use(s)

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Kurada and White, 1998)

visible

(Kon et al., 2005, Shamloula et al., 2002, Smith et al., 2002)

Phenotype Manifest In

embryo

(Kurada and White, 1998)

eye

(Behan et al., 2002, Shamloula et al., 2002, Kurada and White, 1998)

ommatidium

(Kon et al., 2005, Smith et al., 2002, Allard et al., 1996)

photoreceptor cell R3

(Allard et al., 1996)

photoreceptor cell R4

(Allard et al., 1996)

photoreceptor cell R7

(Allard et al., 1996, Kauffmann et al., 1996)

Detailed Description

Statement

Reference

Ras85D^N17.hs.2sev heterozygotes have a very mild eye phenotype.

(Behan et al., 2002)

Flies expressing Ras85D^N17.hs.2sev have a rough eye phenotype.

(Shamloula et al., 2002)

Flies carrying Ras85D^N17.hs.2sev have a rough eye phenotype.

(Smith et al., 2002)

Expression in the embryo causes ectopic cell death.

(Kurada and White, 1998)

60% of the ommatidia of flies carrying Ras85D^N17.hs.2sev lack an R7 cell. Many ommatidia lack one or both of photoreceptors R3 and R4. Ras85D^N17.hs.2sev completely blocks the ability of csw::Src64B^hs.2sev to induce R7 development.

(Allard et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ras85D^N17.hs.2sev has visible phenotype, enhanceable by mask^10.22

(Smith et al., 2002)

NOT Enhanced by

Statement

Reference

Ras85D^N17.hs.2sev has visible phenotype, non-enhanceable by simj[+]/simj^unspecified

(Kon et al., 2005)

Suppressed by

Statement

Reference

Ras85D^N17.hs.2sev has abnormal cell death phenotype, suppressible by Df(3L)H99

(Kurada and White, 1998)

NOT suppressed by

Statement

Reference

Ras85D^N17.hs.2sev has visible phenotype, non-suppressible by simj[+]/simj^unspecified

(Kon et al., 2005)

Enhancer of

Statement

Reference

Ras85D^N17.hs.2sev is an enhancer of visible phenotype of lz^ts1

(Behan et al., 2002)

Ras85D^N17.hs.2sev is an enhancer of visible phenotype of rg^γ6

(Shamloula et al., 2002)

Other

Statement

Reference

Ras85D^N17.hs.2sev, sev^S11.Tag:MYC has viable phenotype

(Maixner et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^N17.hs.2sev has ommatidium phenotype, enhanceable by mask^10.22

(Smith et al., 2002)

NOT Enhanced by

Statement

Reference

Ras85D^N17.hs.2sev has ommatidium phenotype, non-enhanceable by simj[+]/simj^unspecified

(Kon et al., 2005)

NOT suppressed by

Statement

Reference

Ras85D^N17.hs.2sev has ommatidium phenotype, non-suppressible by simj[+]/simj^unspecified

(Kon et al., 2005)

Enhancer of

Statement

Reference

Ras85D^N17.hs.2sev is an enhancer of eye phenotype of lz^ts1

(Behan et al., 2002)

Ras85D^N17.hs.2sev is an enhancer of eye phenotype of rg^γ6

(Shamloula et al., 2002)

Ras85D^N17.hs.2sev is an enhancer of eye phenotype of hid^GMR.PG

(Kurada and White, 1998)

Suppressor of

Statement

Reference

Ras85D^N17.hs.2sev is a suppressor of photoreceptor cell R7 | ectopic phenotype of sty^F7

(Kramer et al., 1999)

Other

Statement

Reference

Ras85D^N17.hs.2sev, lz^r1/lz[+] has eye phenotype

(Behan et al., 2002)

Ras85D^N17.hs.2sev, lz^r1/lz[+] has ommatidium phenotype

(Behan et al., 2002)

Ras85D^N17.hs.2sev, lz^r1 has ommatidium phenotype

(Behan et al., 2002)

Ras85D^N17.hs.2sev, lz^r1 has eye phenotype

(Behan et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

lz^r1/+ ; Ras85D^N17.hs.2sev/+ flies have a rough eye phenotype. Ras85D^N17.hs.2sev enhances the lz^ts1 eye phenotype, making the eye rough at the permissive temperature for lz^ts1 and severely defective at 29^oC.

(Behan et al., 2002)

One copy of mask^10.22 weakly enhances the Ras85D^N17.hs.2sev rough eye phenotype.

(Smith et al., 2002)

The extra R7 cells of sty^F7 are suppressed in ommatidia also mutant for Ras85D^N17.hs.2sev.

(Kramer et al., 1999)

Enhances the eye reduction phenotype caused by W^GMR.PG. The ectopic cell death caused by Ras85D^N17.hs.2sev expression in the embryo is blocked if the embryos are also homozygous for Df(3L)H99.

(Kurada and White, 1998)

Not lethal in combination with sev^{S11.T:Hsap\MYC}.

(Maixner et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5777

P{sevhs-Ras1^N17}JA1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ras85D^N17.hs.2sev

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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