FlyBase Allele Report: Dmel\lz[L]

General Information

Symbol

Dmel\lz^L

Species

D. melanogaster

Name

FlyBase ID

FBal0061066

Feature type

allele

Associated gene

Dmel\lz

Associated Insertion(s)

roo{}lz^L

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Crew et al., 1997)

Mutagen

Progenitor genotype

Associated Insertion(s)

roo{}lz^L

Cytology

Description

Contains a partial, 5-7kb fragment of the roo element located between exons II and III, along with a deletion of 16497bp downstream from this site.

(Jackson Behan et al., 2005)

Deletion from within intron 2 to exon 6 of lz (deletion corresponds to nucleotides 9799-26294 of accession number AJ217651). An LTR-like retrotransposon of approximately 8kb is also inserted at the site of the deletion.

(Siddall et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:9,287,052..9,303,547 [+]

Linked to:

5L-3L restriction fragment

Comment:

Deleted sequence that occurs in conjunction with an 8kb LTR like insertion.

(Siddall et al., 2003)

transposable_element_insertion_site

X:9,287,052..9,287,052 [+]

(Jackson Behan et al., 2005, Siddall et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Siddall et al., 2003)

visible

(Kondo et al., 2009, Crew et al., 1997)

visible | recessive

(Batterham et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

lz^L adults have a "spectacle" eye phenotype. Ommatidial clusters are rarely detectable in lz^L eyes. Ectopic cell death is seen in third larval instar eye discs, between rows 3 an 7 posterior to the morphogenetic furrow.

(Siddall et al., 2003)

15% of photoreceptor cell clusters are abnormal, cells R1 and R6 are not recruited properly. Uniform sheet of unorganised cone cells is found. The periphery of the retina contains cells in loosely organised clusters including cones as well as primary and secondary pigment cells, the darker pigment produces the 'spectacle' phenotype.

(Crew et al., 1997)

Homozygotes have a total lack of ommatidial structure in the eye. Pigment is dispersed as a ring around the edge of the eye ("spectacle" phenotype). Few, if any lenses form, and the eye is covered by a cuticle scar. The 'retina' appears to lack pigment cells. Photoreceptor cell orientation is random, and the fenestrated membrane is absent.

(Batterham et al., 1996)

The antennal funiculus is reduced in size in hemizygous males. All large and small basiconic sensilla on the antennal funiculus are missing, and the number of trichoid sensilla on the antennal funiculus is reduced. The number of coeloconic sensilla on the antennal funiculus is normal. The density of the coeloconic sensilla on the antennal funiculus is increased and the density of the trichoid sensilla on the antennal funiculus is decreased compared to wild-type. Most basiconic sensilla on the maxillary palps are normal, although some are reduced in length.

(Stocker et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

lz^L has visible phenotype, suppressible by P{CH1226-43A10}

(Kondo et al., 2009)

lz^L has increased cell death phenotype, suppressible by BacA\p35^GMR.PH

(Siddall et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

lz^L has eye phenotype, suppressible by P{CH1226-43A10}

(Kondo et al., 2009)

lz^L has eye disc phenotype, suppressible by BacA\p35^GMR.PH

(Siddall et al., 2003)

Other

Statement

Reference

BacA\p35^GMR.PH, lz^L has eye phenotype

(Siddall et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Cell death is blocked in lz^L eye discs that are also expressing BacA\p35^GMR.PH. The eye appears slightly rougher (indicative of ommatidia trying but failing to form) in the double mutants than in lz^L single mutants.

(Siddall et al., 2003)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype of lz^L flies is rescued if they are carrying the P{CH1226-43A10} construct (which contains D. pseudoobscura pseudoobscura fosmid sequence that includes the region orthologous to lz).

(Kondo et al., 2009)

Complementation and Rescue Data

Partially complements

lz³⁷

(Stocker et al., 1993)

Fails to complement

lz^y4

(Batterham et al., 1996)

lz^ts1

(Batterham et al., 1996)

lz^s

(Batterham et al., 1996)