FlyBase Allele Report: Dmel\slpr[BS06]

General Information

Symbol

Dmel\slpr^BS06

Species

D. melanogaster

Name

FlyBase ID

FBal0194556

Feature type

allele

Associated gene

Dmel\slpr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Polaski et al., 2006)

Mutagen

ethyl methanesulfonate

(Polaski et al., 2006)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Polaski et al., 2006)

Amino acid replacement: W48term.

(Polaski et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,176,151..8,176,151 [-]

Nucleotide change:

G8176151A

Reported nucleotide change:

G?A

Amino acid change:

W48term | slpr-PA; W48term | slpr-PB; W48term | slpr-PC; W48term | slpr-PD

Reported amino acid change:

W48term

Comment:

TGG to TAG nonsense mutation in codon Trp48.

(Polaski et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response | adult stage

(Gonda et al., 2012)

abnormal stress response | adult stage

(Hsu et al., 2021)

lethal

(Gonda et al., 2012, Baril et al., 2009)

lethal (with slpr⁹²¹)

(Polaski et al., 2006)

lethal - all die during embryonic stage, with Scer\GAL4^arm.PS, slpr^{D314Y.KM.UASp}

(Polaski et al., 2006)

lethal - all die during embryonic stage | recessive | rescuable maternal effect

(Polaski et al., 2006)

partially lethal (with slpr^3P5)

(Polaski et al., 2006)

partially lethal - majority die

(Stronach et al., 2014)

partially lethal - majority live

(Polaski et al., 2006)

short lived

(Gonda et al., 2012)

some die during embryonic stage, with Scer\GAL4^arm.PS, slpr^{D314Y.KM.UASp}

(Polaski et al., 2006)

some die during embryonic stage | rescuable maternal effect

(Polaski et al., 2006)

visible

(Polaski et al., 2006)

visible (with slpr^3P5)

(Polaski et al., 2006)

visible (with slpr⁹²¹)

(Polaski et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant adults are more susceptible to sustained 37[o]C heat shock than wild-type flies.

Hemizygous males and homozygous females have a significantly reduced lifespan compared to controls, with a median survival of only 14-15 days compared to 50-57 days for wild type.

(Gonda et al., 2012)

Very few slpr^BS06/Y males reach adulthood.

(Baril et al., 2009)

slpr^BS06/Y mutants (lacking zygotic slpr function) have a low rate of embryonic lethality and a variable lethal phase; some die during the pupal stage, but approximately 10% of the expected number of adults eclose. Mutant embryos lacking both zygotic and maternal slpr function show a dramatically increased embryonic lethality and severe dorsal open phenotype. The maternal effect on lethality can be rescued by a slpr⁺ paternal chromosome.

Expression of slpr^{D314Y.KM.Scer\UAS.P\T} under the control of Scer\GAL4^arm.PS in embryos derived from slpr^BS06/+ females significantly increases the percentage of embryonic lethality and severity of cuticle defects compared to embryos expressing slpr^{D314Y.KM.Scer\UAS.P\T} under the control of Scer\GAL4^arm.PS in an otherwise wild-type background.

slpr^BS06 male escapers have a number of morphological defects, which are incompletely penetrant. These defects include crumpled wings (42%), genital rotation and eversion defects (37%), dorsal abdominal clefts or segmental fusions (30%), missing or deformed maxillary palps (35%) and rarely, cleft nota (2%).

29% of slpr^BS06/slpr^3P5 escaper adult females have terminalia defects, with truncation of structures posterior to tergite 7, and are unable to defecate or lay eggs. 36% of the escaper females have maxillary palp defects.

Eggs laid by homozygous females have defective, stunted dorsal appendages.

20% of slpr⁹²¹/slpr^BS06 escaper adult females have maxillary palp defects.

(Polaski et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by slpr::Tak1^{TSK.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Stronach et al., 2014)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by Scer\GAL4^arm.PS/slpr::Tak1^{TSAAA.UASp.Tag:HA}

(Stronach et al., 2014)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by Scer\GAL4^arm.PS/Tak1^{Ct.UASp.Tag:HA}

(Stronach et al., 2014)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by Scer\GAL4^arm.PS/slpr::Tak1^{STCt.UASp.Tag:HA}

(Stronach et al., 2014)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by Scer\GAL4^arm.PS/slpr::Tak1^{STK.UASp.Tag:HA}

(Stronach et al., 2014)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by slpr::Tak1^{SAAATCt.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Stronach et al., 2014)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by kay¹/kay[+]

(Polaski et al., 2006)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by bsk¹/bsk[+]

(Polaski et al., 2006)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(Polaski et al., 2006)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by msn¹⁷²/msn[+]

(Polaski et al., 2006)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by Tak1[+]/Tak1²⁵²⁷

(Polaski et al., 2006)

slpr^BS06 has partially lethal - majority die phenotype, enhanceable by Tak1^K46R.M.UAS/Scer\GAL4^arm.PS

(Polaski et al., 2006)

NOT Enhanced by

Statement

Reference

slpr^BS06 has partially lethal - majority die phenotype, non-enhanceable by Rho1^72O/Rho1[+]

(Polaski et al., 2006)

Suppressed by

Statement

Reference

slpr^BS06 has lethal phenotype, suppressible by puc^E69/puc[+]

(Baril et al., 2009)

slpr^BS06 has lethal phenotype, suppressible by Df(3R)Dr-rv1/+

(Baril et al., 2009)

slpr^BS06 has lethal phenotype, suppressible by alph^XS-88/alph[+]

(Baril et al., 2009)

slpr^BS06 has lethal phenotype, suppressible by alph^S-331/alph[+]

(Baril et al., 2009)

slpr^BS06 has lethal phenotype, suppressible by alph^S-355/alph[+]

(Baril et al., 2009)

slpr^BS06 has partially lethal - majority die phenotype, suppressible by raw¹/raw[+]

(Polaski et al., 2006)

slpr^BS06 has partially lethal - majority die phenotype, suppressible by puc^E69/puc[+]

(Polaski et al., 2006)

slpr^BS06 has partially lethal - majority die phenotype, suppressible | partially by raw⁰²⁴¹⁸/raw[+]

(Polaski et al., 2006)

slpr^BS06 has partially lethal - majority die phenotype, suppressible | partially by aop[+]/aop¹

(Polaski et al., 2006)

NOT suppressed by

Statement

Reference

slpr^BS06 has lethal phenotype, non-suppressible by rl[+]/rl¹

(Baril et al., 2009)

slpr^BS06 has lethal phenotype, non-suppressible by rl[+]/rl^Sem

(Baril et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of slpr::Tak1^{STCt.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS partially rescues the lethality associated with slpr^BS06 mutant males.

Expression of slpr::Tak1^{STK.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS further enhances the lethality associated with slpr^BS06 mutant males.

Expression of slpr::Tak1^{SAAATCt.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS further enhances the lethality associated with slpr^BS06 mutant males.

Expression of slpr::Tak1^{TSK.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS further enhances the lethality associated with slpr^BS06 mutant males.

Expression of slpr::Tak1^{TSAAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS further enhances the lethality associated with slpr^BS06 mutant males.

Expression of Tak1^{Ct.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS further enhances the lethality associated with slpr^BS06 mutant males.

(Stronach et al., 2014)

Df(3R)Dr-rv1/+ restores viability to slpr^BS06/Y males.

alph^XS-88/+, alph^S-331/+ or alph^S-355 restores viability to slpr^BS06/Y males.

puc^E69/+ restores viability to slpr^BS06/Y males.

rl¹/+ or rl^Sem/+ does not suppress the lethality of slpr^BS06/Y males.

(Baril et al., 2009)

The weak lethal phenotype seen in slpr^BS06/Y mutants is enhanced by Tak1²⁵²⁷/+, such that no double mutant adults eclose and there is a slight increase in the relative embryonic lethality and dorsal closure defects of double mutant embryos.

Expression of Tak1^{K46R.M.Scer\UAS} under the control of Scer\GAL4^arm.PS significantly increases the percentage of embryonic lethality and severity of cuticle defects of embryos derived from slpr^BS06/+ females.

(Polaski et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

slpr^BS06 is rescued by Scer\GAL4^arm.PS/slpr^UASp.Tag:HA

(Stronach et al., 2014)

slpr^BS06 is rescued by Scer\GAL4^arm.PS/slpr^{SKLC.UASp.Tag:HA}

(Stronach et al., 2014)

slpr^BS06 is rescued by Scer\GAL4^arm.PS/slpr^UASp.Tag:HA

(Gonda et al., 2012)

slpr^BS06 is rescued by slpr^{PXAP.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Gonda et al., 2012)

slpr^BS06 is rescued by slpr^{PXEP.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Gonda et al., 2012)

slpr^BS06 is rescued by slpr^UASp.cPa/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr^BS06 is rescued by slpr^+tSa

(Polaski et al., 2006)

slpr^BS06 is rescued by slpr^UASp.cPa/Scer\GAL4^arm.PS

(Polaski et al., 2006)

Partially rescued by

slpr^BS06 is partially rescued by Scer\GAL4^arm.PS/slpr^UASp.Tag:HA

(Garlena et al., 2010)

slpr^BS06 is partially rescued by Scer\GAL4^arm.PS/slpr^{SKLC.UASp.Tag:HA}

(Garlena et al., 2010)

slpr^BS06 is partially rescued by slpr^{ΔSH3.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

Not rescued by

slpr^BS06 is not rescued by slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Gonda et al., 2012)

slpr^BS06 is not rescued by slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr^BS06 is not rescued by slpr^{TAA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr^BS06 is not rescued by slpr^{ASA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr^BS06 is not rescued by slpr^{AAT.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr^BS06 is not rescued by slpr^{SKLC.AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr^BS06 is not rescued by slpr^{SH3.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr^BS06 is not rescued by Scer\GAL4^arm.PS/slpr^{Cterm.UASp.Tag:HA}

(Garlena et al., 2010)

slpr^BS06 is not rescued by Scer\GAL4^arm.PS/slpr^{AVA.UASp.Tag:HA}

(Garlena et al., 2010)

Comments

Expression of slpr^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS rescues the lethality associated with slpr^BS06 mutant males.

Expression of slpr^{SKLC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS partially rescues the lethality associated with slpr^BS06 mutant males.

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS further enhances the lethality associated with slpr^BS06 mutant males.

(Stronach et al., 2014)

Expression of either slpr^{PXEP.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{PXAP.Scer\UAS.P\T.T:Ivir\HA1} or slpr^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS significantly rescues the lethality of slpr^BS06 animals.

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS fails to rescue the lethality of slpr^BS06 animals.

(Gonda et al., 2012)

The lethality of slpr^BS06 mutants is completely rescued by expression of slpr^{Scer\UAS.P\T.cPa} under the control of Scer\GAL4^arm.PS.

The lethality of slpr^BS06 mutants is partially rescued by expression of slpr^{Scer\UAS.P\T.T:Ivir\HA1}, slpr^{ΔSH3.Scer\UAS.P\T.T:Ivir\HA1} or slpr^{SKLC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS.

The lethality of slpr^BS06 mutants is not rescued by expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{TAA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{ASA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{AAT.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{SKLC.AAA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{SH3.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{AVA.Scer\UAS.P\T.T:Ivir\HA1} or slpr^{Cterm.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS.

(Garlena et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58807

w¹¹¹⁸ slpr^BS06 P{neoFRT}19A/FM7c, sn⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

slpr^B506

(Deichsel et al., 2024)

slpr^BS06

(Hsu et al., 2021, Stronach et al., 2014, Gonda et al., 2012, Garlena et al., 2010, Lesch et al., 2010, Xiong et al., 2010, Baril et al., 2009, Stronach et al., 2007, Polaski et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (10)

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