FlyBase Allele Report: Dmel\slpr[921]

General Information

Symbol

Dmel\slpr⁹²¹

Species

D. melanogaster

Name

FlyBase ID

FBal0134000

Feature type

allele

Associated gene

Dmel\slpr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Polaski et al., 2006)

Amino acid replacement: E324K.

(Polaski et al., 2006)

Amino acid replacement: E324K. Nucleotide substitution: G?A.

(Stronach, 2006.5.26)

Amino acid replacement: E?K. Mutation is within the kinase domain.

(Stronach and Perrimon, 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,175,198..8,175,198 [-]

Nucleotide change:

G8175198A

Reported nucleotide change:

G?A

Amino acid change:

E324K | slpr-PA; E324K | slpr-PB; E324K | slpr-PC; E324K | slpr-PD

Reported amino acid change:

E324K

Reported amino acid change:

E?K

(Polaski et al., 2006, Stronach, 2006.5.26, Stronach and Perrimon, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

100% of mutant embryos have a severe dorsal open phenotype.

(Garlena et al., 2010)

slpr⁹²¹ displays complete embryonic lethality - dead embryos exhibit a high proportion of severe 'dorsal open' (a hole in the dorsal larval cuticle) phenotypes.

(Baril et al., 2009)

slpr⁹²¹/Y mutants (lacking zygotic slpr function) are embryonic lethal with a severe dorsal closure phenotype. Mutant embryos derived from homozygous female germline clones (lacking both zygotic and maternal slpr function) show the same cuticle phenotype as zygotically mutant embryos.

Eggs laid by females carrying homozygous follicle cell clones have thin, shortened dorsal appendages.

20% of slpr⁹²¹/slpr^BS06 escaper adult females have maxillary palp defects.

(Polaski et al., 2006)

slpr⁹²¹/Y embryos derived from heterozygous females die during embryogenesis with a severe dorsal open phenotype. The concerted elongation of leading edge cells that is seen during dorsal closure initiates correctly in the embryos, but fails shortly afterwards and eventually the cells round up, the dorsal epidermis slackens laterally and the internal organs herniate. A paternal copy of slpr⁺ can rescue embryos derived from females containing slpr⁹²¹ homozygous germ-line clones. Mutant embryos derived from females containing homozygous germ-line clones which do not receive a paternal copy of slpr⁺ die with a severe cuticle phenotype identical to the that of slpr⁹²¹ zygotic mutants.

(Stronach and Perrimon, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

slpr⁹²¹ has lethal - all die during embryonic stage phenotype, suppressible by alph^XS-88/alph^XS-88

(Baril et al., 2009)

slpr⁹²¹ has lethal - all die during embryonic stage phenotype, suppressible by alph^S-331/alph^S-331

(Baril et al., 2009)

Suppressor of

Statement

Reference

slpr⁹²¹/slpr[+] is a suppressor of visible phenotype of Rac1^GMR.PN

(Stronach and Perrimon, 2002)

NOT Suppressor of

Statement

Reference

slpr⁹²¹/slpr[+] is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Polaski et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

slpr⁹²¹ has embryo | dorsal closure stage phenotype, suppressible by Scer\GAL4^arm.PS/slpr::Tak1^{STCt.UASp.Tag:HA}

(Stronach et al., 2014)

slpr⁹²¹ has embryo | dorsal closure stage phenotype, suppressible by Scer\GAL4^arm.PS/slpr::Tak1^{STK.UASp.Tag:HA}

(Stronach et al., 2014)

slpr⁹²¹ has embryonic/first instar larval cuticle | dorsal phenotype, suppressible by alph^XS-88/alph^XS-88

(Baril et al., 2009)

slpr⁹²¹ has embryonic/first instar larval cuticle | dorsal phenotype, suppressible by alph^S-331/alph^S-331

(Baril et al., 2009)

slpr⁹²¹ has embryo | dorsal closure stage phenotype, suppressible by Jra^Asp.hs.sev

(Stronach and Perrimon, 2002)

slpr⁹²¹ has embryo | dorsal closure stage phenotype, suppressible by puc^E69/puc[+]

(Stronach and Perrimon, 2002)

slpr⁹²¹ has embryonic/first instar larval cuticle phenotype, suppressible by puc^E69/puc[+]

(Stronach and Perrimon, 2002)

slpr⁹²¹ has embryonic/first instar larval cuticle phenotype, suppressible by Jra^Asp.hs.sev

(Stronach and Perrimon, 2002)

NOT suppressed by

Statement

Reference

slpr⁹²¹ has embryo | dorsal closure stage phenotype, non-suppressible by slpr::Tak1^{SAAATCt.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Stronach et al., 2014)

slpr⁹²¹ has embryo | dorsal closure stage phenotype, non-suppressible by slpr::Tak1^{TSK.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Stronach et al., 2014)

slpr⁹²¹ has embryo | dorsal closure stage phenotype, non-suppressible by Scer\GAL4^arm.PS/slpr::Tak1^{TSAAA.UASp.Tag:HA}

(Stronach et al., 2014)

Enhancer of

Statement

Reference

slpr⁹²¹ is an enhancer of mesothoracic tergum phenotype of Pvr^RNAi.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

slpr⁹²¹ is an enhancer of thorax phenotype of Pvr^RNAi.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

NOT Enhancer of

Statement

Reference

slpr⁹²¹/slpr[+] is a non-enhancer of eye phenotype of hep^{CA.sev.Tag:MYC}

(Baril et al., 2009)

Suppressor of

Statement

Reference

slpr⁹²¹/slpr[+] is a suppressor of eye phenotype of Rac1^GMR.PN

(Baril et al., 2009, Stronach and Perrimon, 2002)

slpr⁹²¹/slpr[+] is a suppressor of ommatidium phenotype of Rac1^GMR.PN

(Stronach and Perrimon, 2002)

NOT Suppressor of

Statement

Reference

slpr⁹²¹/slpr[+] is a non-suppressor of eye phenotype of hep^{CA.sev.Tag:MYC}

(Baril et al., 2009)

slpr⁹²¹/slpr[+] is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Polaski et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of slpr::Tak1^{STCt.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS partially rescues the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of slpr::Tak1^{STK.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS partially rescues the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of slpr::Tak1^{SAAATCt.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS does not rescue the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of slpr::Tak1^{TSK.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS does not rescue the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of slpr::Tak1^{TSAAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS does not rescue the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of Tak1^{Ct.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS does not rescue the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

(Stronach et al., 2014)

alph^XS-88/alph^XS-88 or alph^S-331/alph^S-331 significantly reduces the number of dead embryos exhibiting a severe 'dorsal open' phenotype, while increasing the proportion the either display a weak 'dorsal open' phenotype or have only head defects.

(Baril et al., 2009)

The eye ablation phenotype seen in adults expressing egr^GS9830 under the control of Scer\GAL4^GMR.PF is not suppressed by slpr⁹²¹/+.

(Polaski et al., 2006)

The severe dorsal open phenotype of slpr⁹²¹/Y embryos is significantly rescued by expression of Jra^Asp.hs.sev (using a 30 minute heat shock several hours before the beginning of dorsal closure) and is also significantly rescued by puc^E69/+.

(Stronach and Perrimon, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

slpr⁹²¹ is rescued by Scer\GAL4^arm.PS/slpr^UASp.Tag:HA

(Stronach et al., 2014)

slpr⁹²¹ is rescued by Scer\GAL4^arm.PS/slpr^{SKLC.UASp.Tag:HA}

(Stronach et al., 2014)

slpr⁹²¹ is rescued by slpr^UASp.cPa/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr⁹²¹ is rescued by slpr^UASp.cPa/Scer\GAL4^arm.PS

(Polaski et al., 2006)

slpr⁹²¹ is rescued by slpr^+tSa

(Stronach and Perrimon, 2002)

Partially rescued by

slpr⁹²¹ is partially rescued by Scer\GAL4^arm.PS/slpr^UASp.Tag:HA

(Garlena et al., 2010)

slpr⁹²¹ is partially rescued by Scer\GAL4^arm.PS/slpr^{SKLC.UASp.Tag:HA}

(Garlena et al., 2010)

slpr⁹²¹ is partially rescued by slpr^{ΔSH3.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

Not rescued by

slpr⁹²¹ is not rescued by slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Stronach et al., 2014)

slpr⁹²¹ is not rescued by slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr⁹²¹ is not rescued by slpr^{TAA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr⁹²¹ is not rescued by slpr^{ASA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr⁹²¹ is not rescued by slpr^{AAT.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr⁹²¹ is not rescued by slpr^{SKLC.AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr⁹²¹ is not rescued by slpr^{SH3.UASp.Tag:HA}/Scer\GAL4^arm.PS

(Garlena et al., 2010)

slpr⁹²¹ is not rescued by Scer\GAL4^arm.PS/slpr^{Cterm.UASp.Tag:HA}

(Garlena et al., 2010)

slpr⁹²¹ is not rescued by Scer\GAL4^arm.PS/slpr^{AVA.UASp.Tag:HA}

(Garlena et al., 2010)

Comments

Expression of slpr^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS rescues the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of slpr^{SKLC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS partially rescues the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS does not rescue the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

(Stronach et al., 2014)

The lethality of slpr⁹²¹ mutants is completely rescued by expression of slpr^{Scer\UAS.P\T.cPa} under the control of Scer\GAL4^arm.PS.

The lethality of slpr⁹²¹ mutants is partially rescued by expression of slpr^{Scer\UAS.P\T.T:Ivir\HA1} or slpr^{SKLC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS.

The lethality of slpr⁹²¹ mutants is not rescued by expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{TAA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{ASA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{SKLC.AAA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{SH3.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{AVA.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{Cterm.Scer\UAS.P\T.T:Ivir\HA1} or slpr^{ΔSH3.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS.

Expression of slpr^{AAT.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS in slpr⁹²¹ mutants results in the recovery of a few rescued adults and many more rescued animals are observed as pharate adults.

Expression of slpr^{SH3.Scer\UAS.P\T.T:Ivir\HA1}, slpr^{SKLC.AAA.Scer\UAS.P\T.T:Ivir\HA1} or slpr^{Cterm.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS fails to rescue the severe dorsal open phenotype of slpr⁹²¹ embryos.

Expression of slpr^{SKLC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS significantly rescues the severe dorsal open phenotype of slpr⁹²¹ embryos (the penetrance of the phenotype is reduced from 100% to approximately 30%).

Expression of slpr^{ΔSH3.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS significantly rescues the severe dorsal open phenotype of slpr⁹²¹ embryos (the penetrance of the phenotype is reduced from 100% to approximately 20%).

Expression of slpr^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS significantly rescues the severe dorsal open phenotype of slpr⁹²¹ embryos (the penetrance of the phenotype is reduced from 100% to approximately 10%).

(Garlena et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58794

y¹ w^* slpr⁹²¹ v¹ P{FRT(w^hs)}101/FM7c, P{ftz-lacC}YH1, sn⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

921

(Stronach and Perrimon, 2002)

slpr⁹²¹

(Stronach et al., 2014, Garlena et al., 2010, Baril et al., 2009, Polaski et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

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