FlyBase Allele Report: Dmel\alph[S-331]

General Information

Symbol

Dmel\alph^S-331

Species

D. melanogaster

Name

FlyBase ID

FBal0120641

Feature type

allele

Associated gene

Dmel\alph

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: G120E.

(Baril and Therrien, 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:29,593,709..29,593,709 [+]

Nucleotide change:

G29593709A

Amino acid change:

Reported amino acid change:

G120E

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Baril and Therrien, 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oxidative stress response

(Baril et al., 2009)

long lived

(Baril et al., 2009)

partially lethal - majority live (with alph^S-355)

(Therrien et al., 2000)

radiation sensitive

(Baril et al., 2009)

viable

(Baril and Therrien, 2006, Therrien et al., 2000)

visible (with alph^S-355)

(Therrien et al., 2000)

wild-type

(Therrien et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygotes show increased tolerance to paraquat and increased longevity compared to controls.

Homozygotes display enhanced adult eye tissue loss following UVC irradiation of 24-hr pupal eyes.

(Baril et al., 2009)

alph^S-331 mutants exhibit a low (~4%) but significant number of extra R7 cells. There are additional or missing cone cells in approximately 5% of the alph^S-331 ommatidia. Mispositioned primary pigment cells as well as additional secondary and tertiary pigment cells are also observed. Three out of four ommatidia with a missing cone cell also exhibit an extra R7-like cells, thereby strongly suggesting that cone cell to R7 cell transformation is taking place.

(Baril and Therrien, 2006)

Homozygotes show no apparent phenotype. Heterozygotes with pp2c99B^S-355 are sub-viable with rough eyes and defects including missing wing veins and atrophied wings.

(Therrien et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

alph^S-331 has radiation sensitive phenotype, suppressible by hep[+]/hep^G0107

(Baril et al., 2009)

Enhancer of

Statement

Reference

pp2c99B[+]/alph^S-331 is an enhancer of visible phenotype of Ras85D^V12.sev

(Therrien et al., 2000)

Suppressor of

Statement

Reference

alph^S-331/alph^S-331 is a suppressor of lethal phenotype of slpr^3P5

(Baril et al., 2009)

alph^S-331/alph^S-331 is a suppressor of lethal - all die during embryonic stage phenotype of slpr⁹²¹

(Baril et al., 2009)

alph^S-331/alph[+] is a suppressor of lethal phenotype of slpr^BS06

(Baril et al., 2009)

alph^S-331/alph^S-331 is a suppressor of lethal - all die during P-stage phenotype of hep^G0107

(Baril et al., 2009)

alph^S-331/alph[+] is a suppressor | partially of lethal - all die during P-stage phenotype of hep^G0107

(Baril et al., 2009)

alph^S-331/alph^S-331 is a suppressor of lethal phenotype of lic^G0252

(Baril et al., 2009)

alph^S-331/alph[+] is a suppressor | partially of lethal phenotype of lic^G0252

(Baril et al., 2009)

pp2c99B[+]/alph^S-331 is a suppressor of lethal phenotype of Raf¹²

(Therrien et al., 2000)

pp2c99B[+]/alph^S-331 is a suppressor of visible phenotype of ksr::tor^{ΔNksr.hs.sev}

(Therrien et al., 2000)

Other

Statement

Reference

PTP-ER^XE-3022, alph^S-331 has lethal phenotype

(Baril and Therrien, 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

alph^S-331 has photoreceptor cell R7 | increased number phenotype, enhanceable by PTP-ER^XE-3022/PTP-ER^XE-2776

(Baril and Therrien, 2006)

alph^S-331 has photoreceptor cell R7 | increased number phenotype, enhanceable by RasGAP1^A13D/Gap1[+]

(Baril and Therrien, 2006)

alph^S-331 has photoreceptor cell R7 | increased number phenotype, enhanceable by rl^Sem.sev

(Baril and Therrien, 2006)

Enhancer of

Statement

Reference

alph^S-331/alph[+] is an enhancer of adult thorax phenotype of Scer\GAL4^pnr-MD237, slpr^UASp.cPa

(Baril et al., 2009)

alph^S-331/alph[+] is an enhancer of scutellum phenotype of Scer\GAL4^pnr-MD237, slpr^UASp.cPa

(Baril et al., 2009)

alph^S-331/alph[+] is an enhancer of scutellar bristle phenotype of Scer\GAL4^pnr-MD237, slpr^UASp.cPa

(Baril et al., 2009)

alph^S-331/alph[+] is an enhancer of eye phenotype of Rac1^GMR.PN

(Baril et al., 2009)

alph^S-331 is an enhancer of photoreceptor cell R7 | increased number phenotype of PTP-ER^XE-3022/PTP-ER^XE-2776

(Baril and Therrien, 2006)

alph^S-331 is an enhancer of photoreceptor cell R7 | increased number phenotype of rl^Sem.sev

(Baril and Therrien, 2006)

pp2c99B[+]/alph^S-331 is an enhancer of photoreceptor cell R7 | increased number phenotype of aop^A141

(Baril and Therrien, 2006)

alph^S-331 is an enhancer of photoreceptor cell R7 | increased number phenotype of aop^A141

(Baril and Therrien, 2006)

pp2c99B[+]/alph^S-331 is an enhancer of eye phenotype of Ras85D^V12.sev

(Therrien et al., 2000)

NOT Enhancer of

Statement

Reference

alph^S-331/alph[+] is a non-enhancer of eye phenotype of hep^{CA.sev.Tag:MYC}

(Baril et al., 2009)

alph^S-331/alph[+] is a non-enhancer of eye phenotype of lic^{CA.sev.Tag:MYC}

(Baril et al., 2009)

alph^S-331 is a non-enhancer of eye phenotype of phyl^hs.sev

(Baril and Therrien, 2006)

Suppressor of

Statement

Reference

alph^S-331/alph^S-331 is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of slpr⁹²¹

(Baril et al., 2009)

alph^S-331 is a suppressor of photoreceptor neuron phenotype of ksr::tor^{ΔNksr.hs.sev}

(Baril and Therrien, 2006)

pp2c99B[+]/alph^S-331 is a suppressor of eye phenotype of ksr::tor^{ΔNksr.hs.sev}

(Therrien et al., 2000)

NOT Suppressor of

Statement

Reference

alph^S-331/alph[+] is a non-suppressor of eye phenotype of hep^{CA.sev.Tag:MYC}

(Baril et al., 2009)

alph^S-331/alph[+] is a non-suppressor of eye phenotype of lic^{CA.sev.Tag:MYC}

(Baril et al., 2009)

Other

Statement

Reference

RasGAP1^A13D/Gap1[+], alph^S-331 has wing vein phenotype

(Baril and Therrien, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Introduction of a homozygous alph^S-331 allele into the lic^G0252 background strongly suppresses hemizygous male lethality.

Introduction of a heterozygous alph^S-331 allele into the lic^G0252 background suppresses hemizygous male lethality.

Introduction of a homozygous alph^S-331 allele into the hep^G0107 background strongly suppresses hemizygous male lethality.

Introduction of a heterozygous alph^S-331 allele into the hep^G0107 background suppresses hemizygous male lethality.

alph^S-331/+ restores viability to slpr^BS06/Y males.

alph^S-331/alph^S-331 significantly reduces the number of dead embryos exhibiting a severe 'dorsal open' phenotype, while increasing the proportion the either display a weak 'dorsal open' phenotype or have only head defects.

alph^S-331/+ enhances the thorax closure defects of Scer\GAL4^pnr-MD237 slpr^{Scer\UAS.P\T.cPa} flies - the scutellum is smaller, and more bristles are lost.

hep^G0107/+ suppresses the eye loss enhancement seen in alph^S-331 homozygotes following UVC irradiation of 24-hr pupal eyes.

(Baril et al., 2009)

Homozygous aop^A141 mutants, with one copy of alph^S-331 exhibit an increase from 37% to 86% in the number of extra R7 cells. Double homozygotes exhibit extra R7 cells in all ommatidia, with an average of almost three R7 cells per ommatidium, which visibly increases eye roughness.

PTP-ER^XE-3022, in a double homozygote combination with alph^S-331 exhibits a synthetic lethality.

PTP-ER^XE-3022/PTP-ER^XE-2776 do not show synthetic lethality when placed in an alph^S-331 homozygous background, however, a significant increase of extra R7 cells is observed compared to the respective single homozygotes.

Introduction of Gap1^A13D/+ into a homozygous alph^S-331 background not only increases the number of extra R7 cells, but also induces the formation of additional wing vein material.

rl^Sem.sev heterozygous mutants in combination with alph^S-331 exhibit a rough eye phenotype. They exhibit an average of 2.75 R7 cells per ommatidium, compared to 1 R7 cell per ommatidium in wild-type.

ksr::tor^{ΔNksr.hs.sev}/alph^S-331 mutants exhibit a 24% loss of ommatidial R7 cells, indicating that alph^S-331 has suppressed the ksr::tor^{ΔNksr.hs.sev} phenotype. This effect is not specific to the R7 cells as the loss of outer photoreceptors is suppressed in this double mutant.

phyl^hs.sev heterozygous mutants in combination with alph^S-331 exhibit a rough eye phenotype. They exhibit an average of 1.72 R7 cells per ommatidium, compared to 1 R7 cell per ommatidium in wild-type. This however is no higher than in phyl^hs.sev heterozygous mutants.

(Baril and Therrien, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

alph^S-355

(Therrien et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59009

alph^S-331

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

S-331

(Baril and Therrien, 2006)

SK3-1^S-331

(Therrien et al., 2000)

alph^S-331

(Baril et al., 2009, Baril and Therrien, 2006)

pp2c99B^S-331

Name Synonyms

Secondary FlyBase IDs

References (3)

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