Date: Thu, 25 May 2006 14:45:16 \-0400 From: beth stronach <stronach+@pitt.edu> Subject: Re: Request for info on slpr mutations for FlyBase To: Beverley Matthews <bmatthew@XXXX> Hi Beverley: please see attached WORD doc. these details are going to be published in a new manuscript coming out in Genetics with info on a new slpr allele. The numbers are based on the protein starting with the second ATG codon, which fits the consensus much better for translation initiation. That protein would be 1148 aa in total. Let me know if you need anything else. Beth >Dear Dr. Stronach, > >I initially sent my request to Norbert as corresponding author >and he asked me to forward it along to you so here it is. > >I would like to curate the locations of the mutations reported in > >FBrf0144880 == Stronach and Perrimon, 2002, Genes Dev. 16(3): 377--387 > >to appear in the genome maps in FlyBase but need more information. >Can you provide me with the amino acid positions of the slpr3P5 >and slpr921 mutations and let me know if slpr3P5 is a TGG \-> TAG >or a TGG \-> TGA mutation? > >Thanks in advance for any help you can offer. Your response will >be saved as a Personal Communication to FlyBase and used as a >reference for the mapping of the mutations (in addition to the >Genes Dev. paper). > >Sincerely, > >Beverley Matthews >Curator >FlyBase-Harvard