UASp regulatory sequences drive expression of amino acids 1-516 of slpr followed by amino acids 272-678 from Tak1. Three residues in the slpr activation loop have been mutated to alanine to prevent activating phosphorylation (T287A, S291A and T295A). The construct is tagged at the C-terminal end with two copies of Tag:HA.
slpr::Tak1SAAATCt.UASp.Tag:HA/Scer\GAL4arm.PS is an enhancer of partially lethal - majority die phenotype of slprBS06
slpr::Tak1SAAATCt.UASp.Tag:HA/Scer\GAL4GMR.PF is a suppressor of visible phenotype of Scer\GAL4GMR.PF, egrGS9830
slpr::Tak1SAAATCt.UASp.Tag:HA/Scer\GAL4da.G32 is a non-suppressor of abnormal immune response phenotype of Tak12
slpr::Tak1SAAATCt.UASp.Tag:HA/Scer\GAL4GMR.PF is a suppressor of eye phenotype of Scer\GAL4GMR.PF, egrGS9830
slpr::Tak1SAAATCt.UASp.Tag:HA/Scer\GAL4arm.PS is a non-suppressor of embryo | dorsal closure stage phenotype of slpr921
Expression of slpr::Tak1SAAATCt.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS further enhances the lethality associated with slprBS06 mutant males.
Expression of slpr::Tak1SAAATCt.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS does not rescue the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.
Expression of slpr::Tak1SAAATCt.Scer\UAS.P\T.T:Ivir\HA1 suppresses the reduction in eye size seen when egrGS9830 is expressed under the control of Scer\GAL4GMR.PF.
Expression of slpr::Tak1SAAATCt.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4da.G32 does not suppress the increased susceptibility to E.coli challenge seen in Tak12/Y males. Expression of slpr::Tak1SAAATCt.Scer\UAS.P\T.T:Ivir\HA1 modestly increases susceptibility of Tak12/+ females to E.coli.