FlyBase Allele Report: Dmel\N[unspecified]

General Information

Symbol

Dmel\N^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0089580

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage (with N^Nul)

(Dambly-Chaudiere and Leyns, 1992)

lethal - all die before end of P-stage | heat sensitive, with N^gt-10-15

(Leonardi et al., 2011)

neurogenic phenotype

(Tremmel et al., 2013)

some die during larval stage (with N^Nul)

(Dambly-Chaudiere and Leyns, 1992)

some die during pharate adult stage | heat sensitive, with N^gt-10-15

(Leonardi et al., 2011)

visible, with N^gt-10-20

(Leonardi et al., 2011)

visible, with N^gt-10-35

(Leonardi et al., 2011)

visible, with N^gt-16-35

(Leonardi et al., 2011)

visible | heat sensitive, with N^gt-10-15

(Leonardi et al., 2011)

visible | heat sensitive, with N^{gt-10.12.14.15}

(Leonardi et al., 2011)

Phenotype Manifest In

cardioblast

(Haag et al., 1999)

eye disc & neuron | somatic clone

(Ligoxygakis et al., 1998)

larval RP2 motor neuron | ectopic

(Rusconi and Corbin, 1999)

leg | heat sensitive, with N^gt-10-15

(Leonardi et al., 2011)

leg joint, with N^gt-10-20

(Leonardi et al., 2011)

leg joint, with N^gt-10-35

(Leonardi et al., 2011)

leg joint | heat sensitive, with N^gt-10-15

(Leonardi et al., 2011)

leg joint | heat sensitive, with N^{gt-10.12.14.15}

(Leonardi et al., 2011)

notal microchaeta, with N^gt-10-20

(Leonardi et al., 2011)

notal microchaeta, with N^gt-10-35

(Leonardi et al., 2011)

notal microchaeta, with N^gt-16-35

(Leonardi et al., 2011)

notal microchaeta | heat sensitive, with N^gt-10-15

(Leonardi et al., 2011)

notal microchaeta | heat sensitive, with N^{gt-10.12.14.15}

(Leonardi et al., 2011)

RP2sib neuron

(Rusconi and Corbin, 1999)

salivary gland

(Lammel and Saumweber, 2000)

wing

(de Celis and Barrio, 2000)

wing vein

(de Celis and Barrio, 2000)

Detailed Description

Statement

Reference

N^gt-10-15 rescues N^unspecified mutants to pharate adulthood at 30 [o]C, but the rescued animals show a severe loss of bristles on the thorax, loss of leg joints and severe leg shortening. When the rescued animals are raised at 25 [o]C, they readily eclose, the severity of bristle loss is significantly decreased and the legs look normal. At lower temperatures (18-23 [o]C), the rescued animals show a normal bristle pattern and normal legs.

Two copies of N^gt-10-15 significantly rescues the bristle and leg phenotypes of a N^unspecified null allele at 30 [o]C, although not completely, because the rescued animals still display some leg joint defects and die as pharate adults.

N^gt-16-35 rescues N^unspecified mutants to pharate adulthood. The rescued animals show only a mild loss of microchaetae on the notum.

N^gt-10-20 rescues N^unspecified mutants to pharate adulthood at room temperature (21-23 [o]C). The rescued animals show an intermediate loss of microchaetae on the notum. Some rescued animals show leg joint defects.

N^gt-10-35 rescues N^unspecified null alleles to pharate adulthood at room temperature (21-23 [o]C). About half of the rescued animals show almost complete loss of microchaetae on the notum and the remainder show a severe loss of microchaetae with some intact bristles. Leg joint defects without leg shortening are present in most of the rescued animals.

(Leonardi et al., 2011)

Mutant embryos have small or absent salivary gland rudiments.

(Lammel and Saumweber, 2000)

All the cells in the vein competent region of the wing differentiate as vein cells, causing a thickening of the vein.

(de Celis and Barrio, 2000)

Reduction of N function during 7-9 hours of development results in a strong increase in the number of cardioblasts, although the cardioblasts still align and meet up with their contralateral counterparts.

(Haag et al., 1999)

In zygotic null embryos, RP2sib neurons become eve expressing RP2 neurons.

(Rusconi and Corbin, 1999)

Homozygous clones in the eye disc lack most neural differentiation.

(Ligoxygakis et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^unspecified has neurogenic phenotype phenotype, suppressible by Pkc98E^f06221

(Tremmel et al., 2013)

N^unspecified, dx^ENU has lethal phenotype, suppressible by Su(dx)⁴

(Fostier et al., 1998)

N^unspecified, dx^ENU has lethal phenotype, suppressible by Su(dx)⁵⁶

(Fostier et al., 1998)

Suppressor of

Statement

Reference

N^unspecified is a suppressor of visible phenotype of Myt1^GMR.PP, Wee1^GMR.PP

(Price et al., 2002)

Other

Statement

Reference

N^unspecified, net^unspecified has visible phenotype

(Biehs et al., 1998)

N^unspecified, net^unspecified has visible | recessive phenotype

(Biehs et al., 1998)

N^unspecified, Su(dx)^sp has partially lethal - majority die phenotype

(Fostier et al., 1998)

N^unspecified, dx^ENU has lethal phenotype

(Fostier et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^unspecified has wing phenotype, enhanceable by M285^M285

(Go and Artavanis-Tsakonas, 1998)

N^unspecified has phenotype, enhanceable by E(nd)195^ry+3550

(Hing and Artavanis-Tsakonas, 1995)

Suppressed by

Statement

Reference

N^unspecified has phenotype, suppressible by Scer\GAL4^da.G32/N::tor^UAS.cSa

(Zecchini et al., 1999)

N^unspecified has phenotype, suppressible by Delta¹

(Siren and Portin, 1989)

Enhancer of

Statement

Reference

N^unspecified, Scer\GAL4^dpp.blk1 is an enhancer of wing | distal phenotype of Scer\GAL4^dpp.blk1, sca^UAS.cEa

(Lee et al., 2000)

Suppressor of

Statement

Reference

N^unspecified is a suppressor of eye phenotype of Myt1^GMR.PP, Wee1^GMR.PP

(Price et al., 2002)

Other

Statement

Reference

N^unspecified, net^unspecified has wing phenotype

(Biehs et al., 1998)

N^unspecified, net^unspecified has wing vein | ectopic phenotype

(Biehs et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The neurogenic phenotype seen in N^unspecified embryos is suppressed specifically in the ventral region in Pkc98E^f06221 N^unspecified double mutant embryos.

(Tremmel et al., 2013)

The non-autonomous overgrowth phenotype caused by Df(1)ph^del clones in the eye is suppressed if the clones are also mutant for N^unspecified, but the autonomous apoptosis and defective differentiation seen in Df(1)ph^del clones in the eye disc is not suppressed in the double mutant clones.

(Feng et al., 2011)

The eye phenotype caused by two copies of Myt1^GMR.PP combined with a single copy of wee^GMR.PP is suppressed by N^unspecified.

(Price et al., 2002)

N^unspecified/+; net^unspecified/net^unspecified flies have solid ectopic veins between L2 and L3 and between L4 and L5.

(Biehs et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

N^unspecified is rescued by N^gt-10.13-15

(Leonardi et al., 2011)

N^unspecified is rescued by N^gt-12

(Leonardi et al., 2011)

N^unspecified is rescued by N^gt-12.14.15

(Leonardi et al., 2011)

N^unspecified is rescued by N^gt-16-20

(Leonardi et al., 2011)

N^unspecified is rescued by N^gt-24-35

(Leonardi et al., 2011)

N^unspecified is rescued by N^gt-4.5

(Leonardi et al., 2011)

N^unspecified is rescued by Scer\GAL4^da.G32/N^FLN.UAS

(Zecchini et al., 1999)

Partially rescued by

N^unspecified is partially rescued by N^gt-10-35

(Leonardi et al., 2011)

N^unspecified is partially rescued by N^gt-10-20

(Leonardi et al., 2011)

N^unspecified is partially rescued by N^gt-10-15

(Leonardi et al., 2011)

N^unspecified is partially rescued by N^{gt-10.12.14.15}

(Leonardi et al., 2011)

N^unspecified is partially rescued by N^gt-16-35

(Leonardi et al., 2011)

N^unspecified is partially rescued by N^int.SH.UAS/Scer\GAL4^da.G32

(Zecchini et al., 1999)

N^unspecified is partially rescued by Scer\GAL4^da.G32/N^{FLN.ΔBRV.UAS}

(Zecchini et al., 1999)

N^unspecified is partially rescued by N^{FLNΔ10-12.UAS}/Scer\GAL4^da.G32

(Zecchini et al., 1999)

N^unspecified is partially rescued by N^mMGIIa

(Ramos et al., 1989)

N^unspecified is partially rescued by N^mMG810

(Ramos et al., 1989)

Not rescued by

N^unspecified is not rescued by N^{FLN.ΔCdc10.UAS}/Scer\GAL4^da.G32

(Zecchini et al., 1999)

Comments

N^gt-12.14.15 fully rescues the N^unspecified null phenotypes even when raised at 30 [o]C.

N^unspecified mutants rescued by N^{gt-10.12.14.15} show mild to moderate bristle loss on the thorax and occasional leg joint defects at 30 [o]C.

N^gt-12 fully rescues the lethality and phenotypes of N^unspecified null mutants at 30 [o]C.

N^gt-10.13-15 is able to rescue the lethality and phenotypes of N^unspecified mutants at 30 [o]C.

N^gt-16-20 fully rescues the bristle and leg phenotypes of N^unspecified alleles at 30 [o]C.

N^gt-16-35 rescues N^unspecified mutants to pharate adulthood. The rescued animals show only a mild loss of microchaetae on the notum.

N^gt-24-35 fully rescues the lethality and phenotypes associated with N^unspecified.

N^gt-4.5 fully rescues the lethality and phenotypes associated with N^unspecified.

(Leonardi et al., 2011)

P-element mediated phenotypic rescue demonstrates that a 40.8kb N fragment from -28.5 to +12.3 on the molecular map is functionally indistinguishable from a duplication of the N locus.

(Ramos et al., 1989)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

N^unspecified

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

Open Close