FlyBase Allele Report: Dmel\N[gt-10-35]

General Information

Symbol

Dmel\N^gt-10-35

Species

D. melanogaster

Name

FlyBase ID

FBal0269857

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{N.gt-10_35}

PBac{N.gt-10_35}

Key Links

Transgenic product class

missense_variant

(Leonardi et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Leonardi et al., 2011)

Mutagen

in vitro construct

(Leonardi et al., 2011)

Progenitor genotype

N^+t40

(Leonardi et al., 2011)

Carried in construct

P{N.gt-10_35}

PBac{N.gt-10_35}

Cytology

Description

Contains serine-to-alanine mutations in the EGF repeats of region 10_35 to abolish the addition of O-glucose.

(Leonardi et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with N^unspecified

(Leonardi et al., 2011)

visible | cold sensitive

(Leonardi et al., 2011)

Phenotype Manifest In

leg joint, with N^unspecified

(Leonardi et al., 2011)

notal microchaeta, with N^unspecified

(Leonardi et al., 2011)

wing vein | cold sensitive

(Leonardi et al., 2011)

Detailed Description

Statement

Reference

N^gt-10-35 rescues N^unspecified null alleles to pharate adulthood at room temperature (21-23 [o]C). About half of the rescued animals show almost complete loss of microchaetae on the notum and the remainder show a severe loss of microchaetae with some intact bristles. Leg joint defects without leg shortening are present in most of the rescued animals.

Flies with two copies of N^gt-10-35 show extra wing vein tissue and wing vein expansion at 18-23 [o]C, but not at higher temperatures.

(Leonardi et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

N^gt-10-35 is a suppressor | partially of visible | recessive phenotype of rumi^Δ26

(Leonardi et al., 2011)

NOT Suppressor of

Statement

Reference

N^gt-10-35 is a non-suppressor of lethal | heat sensitive phenotype of rumi^Δ26

(Leonardi et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

N^gt-10-35 is a suppressor | partially of notal microchaeta phenotype of rumi^Δ26

(Leonardi et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous rumi^Δ26 mutant animals also carrying N^gt-10-35 fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C.

(Leonardi et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

N^gt-10-35 partially rescues N^unspecified

(Leonardi et al., 2011)

Comments

(Leonardi et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

N^gt-10-35

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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