FlyBase Allele Report: Dmel\sca[UAS.cEa]

General Information

Symbol

Dmel\sca^UAS.cEa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ellis

FlyBase ID

FBal0039875

Feature type

allele

Associated gene

Dmel\sca

Associated Insertion(s)

Carried in Construct

P{UAS-sca.E}

Also Known As

UAS-sca

Key Links

Transgenic product class

cDNA

(Ellis et al., 1994)

wild_type

(Ellis et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Ellis et al., 1994)

wild_type

(Ellis et al., 1994)

Mutagen

in vitro construct

(Lee et al., 1998, Ellis et al., 1994)

Progenitor genotype

Carried in construct

P{UAS-sca.E}

Cytology

Description

sca cDNA expressed under regulation of UASt regulatory sequences.

(Ellis et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

sca

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Chou and Chien, 2002)

visible, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004, Lee et al., 1998)

visible, with Scer\GAL4^h-1J3

(Lee et al., 1998, Ellis et al., 1994)

Phenotype Manifest In

eye, with Scer\GAL4^h-1J3

(Ellis et al., 1994)

microchaeta, with Scer\GAL4^pnr-MD237

(Renaud and Simpson, 2001)

ommatidium, with Scer\GAL4^h-1J3

(Ellis et al., 1994)

ommatidium, with Scer\GAL4^hs.2sev

(Chou and Chien, 2002)

wing, with Scer\GAL4^dpp.blk1

(Lee et al., 1998)

wing | distal, with Scer\GAL4^dpp.blk1

(Lee et al., 2000)

wing margin, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004, Li et al., 2003)

wing vein, with Scer\GAL4^h-1J3

(Lee et al., 1998)

Detailed Description

Statement

Reference

Expression of sca^Scer\UAS.cEa under the control of Scer\GAL4^dpp.blk1 causes a loss of wing margin structure.

(Kamimura et al., 2004)

When sca^Scer\UAS.cEa is driven by Scer\GAL4^dpp.blk1 loss of wing margin is seen.

(Li et al., 2003)

Ommatidial clusters in the eye discs of sca^Scer\UAS.cEa; Scer\GAL4^hs.2sev larvae, display an under-rotation defect, with many of them arresting rotation at 40^o-45^o. This defect is unaffected by sca^unspecified/+.

(Chou and Chien, 2002)

When sca^Scer\UAS.cEa is driven by Scer\GAL4^pnr-MD237, causes a slightly disorganised bristle pattern, but there are no naked patches and bristles are fairly evenly spaced. The density of bristles is reduced compared to wild-type.

(Renaud and Simpson, 2001)

Expression of sca^Scer\UAS.cEa when driven by Scer\GAL4^dpp.blk1 causes loss of wing margin structures where the dpp expression intersects the dorsal-ventral boundary. Higher doses of sca^Scer\UAS.cEa causes a more extensive phenotype.

(Lee et al., 2000)

Flies expressing sca^Scer\UAS.cEa under the control of Scer\GAL4^dpp.blk1 have nicked wings. This phenotype is still seen in a sca^BP2 background. Flies expressing sca^Scer\UAS.cEa under the control of Scer\GAL4^h-1J3 show expansion of wing veins into a "delta" shape.

(Lee et al., 1998)

Flies carrying Scer\GAL4^h-1J3 show a rough eye phenotype. Ommatidia are oversized, irregularly spaced and fused. The cause of this phenotype is increased density of preclusters in the morphogenetic furrow.

(Ellis et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, sca^UAS.cEa has visible phenotype, enhanceable by Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

Scer\GAL4^hs.2sev, sca^UAS.cEa has abnormal planar polarity phenotype, enhanceable by nmo^unspecified/nmo[+]

(Chou and Chien, 2002)

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, sca^UAS.cEa has abnormal planar polarity phenotype, suppressible | partially by Scer\GAL4^hs.2sev/nmo^c5-1.UAS

(Chou and Chien, 2002)

Enhancer of

Statement

Reference

sca^UAS.cEa, Scer\GAL4^dpp.blk1 is an enhancer of visible phenotype of Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

Suppressor of

Statement

Reference

sca^UAS.cEa/Scer\GAL4^hs.2sev is a suppressor of abnormal planar polarity | recessive phenotype of hh^bar3

(Chou and Chien, 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, sca^UAS.cEa has wing margin phenotype, enhanceable by Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1, sca^UAS.cEa has wing margin phenotype, enhanceable by Gp150^UAS.cLa, Scer\GAL4^dpp.blk1

(Li et al., 2003)

Scer\GAL4^hs.2sev, sca^UAS.cEa has ommatidium phenotype, enhanceable by nmo^unspecified/nmo[+]

(Chou and Chien, 2002)

Scer\GAL4^dpp.blk1, sca^UAS.cEa has wing | distal phenotype, enhanceable by N^unspecified, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, sca^UAS.cEa has wing margin phenotype, suppressible by Gp150⁴/Gp150³

(Li et al., 2003)

Scer\GAL4^hs.2sev, sca^UAS.cEa has ommatidium phenotype, suppressible | partially by Scer\GAL4^hs.2sev/nmo^c5-1.UAS

(Chou and Chien, 2002)

Scer\GAL4^dpp.blk1, sca^UAS.cEa has wing | distal phenotype, suppressible by N^UAS.cSa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

Enhancer of

Statement

Reference

sca^UAS.cEa, Scer\GAL4^dpp.blk1 is an enhancer of wing vein L3 phenotype of Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

sca^UAS.cEa, Scer\GAL4^dpp.blk1 is an enhancer of wing margin phenotype of Gp150^UAS.cLa, Scer\GAL4^dpp.blk1

(Li et al., 2003)

NOT Enhancer of

Statement

Reference

sca^UAS.cEa, Scer\GAL4^dpp.blk1 is a non-enhancer of anterior crossvein phenotype of N^UAS.cSa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

sca^UAS.cEa, Scer\GAL4^dpp.blk1 is a non-enhancer of wing vein | ectopic phenotype of N^UAS.cSa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

Suppressor of

Statement

Reference

sca^UAS.cEa/Scer\GAL4^hs.2sev is a suppressor of ommatidium phenotype of hh^bar3

(Chou and Chien, 2002)

NOT Suppressor of

Statement

Reference

sca^UAS.cEa, Scer\GAL4^dpp.blk1 is a non-suppressor of anterior crossvein phenotype of N^UAS.cSa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

sca^UAS.cEa, Scer\GAL4^dpp.blk1 is a non-suppressor of wing vein | ectopic phenotype of N^UAS.cSa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

When sca^Scer\UAS.cEa and Gp150^Scer\UAS.cLa are driven by Scer\GAL4^dpp.blk1, there is a loss of wing margin that is stronger than either sca^Scer\UAS.cEa or Gp150^Scer\UAS.cLa on its own. When sca^Scer\UAS.cEa is driven by Scer\GAL4^dpp.blk1 in a Gp150⁴/Gp150⁴ background, no wing margin loss is seen.

(Li et al., 2003)

The under-rotation defect exhibited by the ommatidia in the eye discs of sca^Scer\UAS.cEa; Scer\GAL4^hs.2sev larvae, is enhanced by nmo^unspecified/+, and partially suppressed by nmo^{c5-1.Scer\UAS}.

(Chou and Chien, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^sca-73-1/sca^UAS.cEa partially rescues sca^73-1/sca^BP2

(Lee et al., 1998)

Fails to rescue

Scer\GAL4^pnr-MD237/sca^UAS.cEa fails to rescue sca^BP2

(Renaud and Simpson, 2001)

Comments

Rescues the phenotype of sca^BP2/sca^73-1 flies when expressed under the control of Scer\GAL4^sca-73-1; the eyes are less rough and the number of extra thoracic bristles is reduced, often producing the normal bristle pattern.

(Lee et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sca^Scer\UAS.cEa

sca^UAS.cEa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ellis

(Ellis et al., 1994)

Secondary FlyBase IDs

References (9)

Report Sections

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