FlyBase Allele Report: Dmel\dx[ENU]

General Information

Symbol

Dmel\dx^ENU

Species

D. melanogaster

Name

FlyBase ID

FBal0031145

Feature type

allele

Associated gene

Dmel\dx

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl nitrosourea

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Fuwa et al., 2006)

Mutagen

ethyl nitrosourea

(Xu and Artavanis-Tsakonas, 1990)

Progenitor genotype

Cytology

Description

Amino acid replacement: E157K.

(Fuwa et al., 2006)

Nucleotide substitution: ?469?.

(Fuwa et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:6,534,884..6,534,884 [+]

Nucleotide change:

G6534884A

Reported nucleotide change:

?469?

Amino acid change:

E158K | dx-PA

Reported amino acid change:

E157K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Fuwa et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless | recessive

(Xu and Artavanis-Tsakonas, 1990)

lethal | maternal effect | recessive

(Gorman and Girton, 1992)

visible

(Fuwa et al., 2006, Ramain et al., 2001, Fostier et al., 1998)

visible (with dx¹⁵²)

(Fuwa et al., 2006)

visible | recessive

(Xu and Artavanis-Tsakonas, 1990)

Phenotype Manifest In

eye

(Busseau et al., 1994)

microchaeta

(Ramain et al., 2001)

ocellus

(Busseau et al., 1994)

wing

(Fostier et al., 1998, Busseau et al., 1994)

wing (with dx¹⁵²)

(Fuwa et al., 2006)

wing vein

(Fuwa et al., 2006, Fostier et al., 1998)

wing vein (with dx¹⁵²)

(Fuwa et al., 2006)

Detailed Description

Statement

Reference

Homozygous and dx^ENU/dx¹⁵² flies show wing vein thickening, with the severity of the phenotype being stronger in the dx^ENU/dx¹⁵² flies (homozygotes showing only a weak wing vein phenotype).

(Fuwa et al., 2006)

Homozygous females have an excess of microchaetae, having 133.13 +/- 1.88 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54).

(Ramain et al., 2001)

Thickened wing veins and wing margin loss.

(Fostier et al., 1998)

Viable. Extra wing vein material at distal ends, nicked margins an tips. Ocelli are fused or closed, hairs and bristles are abnoral or missing. Weak rough eye phenotype: irregular ommatidia, bristles are duplicated or missing. No visual abnormality. Homozygotes rescue the mutant phenotype of N^Ax-E2, N^Ax-9 and N^Ax-75c transheterozygotes. 40% eggs from homozygous females fail to hatch: defective dorsal appendages and no cuticular structures. Hemizygote males with N^Ax-E2 have a low fertility. N^nd-1/dx^ENU and N^nd-2/dx^ENU transheterozygous flies never reach adulthood (stage of lethality not determined). An extra wild type copy of N suppresses the wing nicks and thickened veins, and partially suppresses the ocellar phenotype. An extra wild type copy of Dl or mam causes some pupal lethality, escapers have small eyes. Flies heterozygous for H and homozygous for dx^ENU have eyes smaller than wild type, infrequent missing or duplicated bristle phenotype and ommatidia are square wih a bristle at each corner.

(Xu and Artavanis-Tsakonas, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^Ax-9/N^Ax-E2, dx^ENU/dx[+] has partially lethal - majority die phenotype, enhanceable by krz^S095214

(Mukherjee et al., 2005)

Suppressed by

Statement

Reference

N^unspecified, dx^ENU has lethal phenotype, suppressible by Su(dx)⁴

(Fostier et al., 1998)

N^unspecified, dx^ENU has lethal phenotype, suppressible by Su(dx)⁵⁶

(Fostier et al., 1998)

Enhancer of

Statement

Reference

dx^ENU/dx[+] is an enhancer of visible | heat sensitive phenotype of dsh^hs.PA

(Ramain et al., 2001)

Suppressor of

Statement

Reference

dx^ENU/dx[+] is a suppressor | partially of partially lethal - majority die phenotype of N^Ax-9/N^Ax-E2

(Mukherjee et al., 2005)

dx^ENU/dx[+] is a suppressor of visible | dominant phenotype of N^Mcd1

(Ramain et al., 2001)

dx^ENU/dx[+] is a suppressor of visible | dominant phenotype of N^Mcd5

(Ramain et al., 2001)

dx^ENU/dx[+] is a suppressor of visible | dominant phenotype of N^Mcd8

(Ramain et al., 2001)

Other

Statement

Reference

N^unspecified, dx^ENU has lethal phenotype

(Fostier et al., 1998)

N^Ax-E2, Su(dx)^sp, dx^ENU has lethal phenotype

(Busseau et al., 1994)

N^Ax-9/N^Ax-E2, Su(dx)[+]/Su(dx)¹, dx^ENU has lethal phenotype

(Busseau et al., 1994)

Phenotype Manifest In

Enhanced by

Statement

Reference

dx^ENU has phenotype, enhanceable by Pcif1^EY11352/CG11399[+]

(Bejarano et al., 2008)

Suppressed by

Statement

Reference

dx^ENU has wing vein phenotype, suppressible by Su(dx)^sp

(Fostier et al., 1998)

dx^ENU has wing phenotype, suppressible by Su(dx)^sp

(Fostier et al., 1998)

dx^ENU has wing vein phenotype, suppressible by Su(dx)⁴

(Fostier et al., 1998)

dx^ENU has wing phenotype, suppressible by Su(dx)⁴

(Fostier et al., 1998)

dx^ENU has wing vein phenotype, suppressible by Su(dx)⁵⁶

(Fostier et al., 1998)

dx^ENU has wing phenotype, suppressible by Su(dx)⁵⁶

(Fostier et al., 1998)

dx^ENU has wing vein phenotype, suppressible by Df(2L)Su(dx)-7

(Fostier et al., 1998)

dx^ENU has wing phenotype, suppressible by Df(2L)Su(dx)-7

(Fostier et al., 1998)

dx^ENU has eye phenotype, suppressible by Su(dx)[+]/Su(dx)¹

(Busseau et al., 1994)

dx^ENU has ocellus phenotype, suppressible by Su(dx)[+]/Su(dx)¹

(Busseau et al., 1994)

dx^ENU has wing phenotype, suppressible by Su(dx)^sp/Su(dx)[+]

(Busseau et al., 1994)

dx^ENU has eye phenotype, suppressible by Su(dx)^sp/Su(dx)[+]

(Busseau et al., 1994)

dx^ENU has ocellus phenotype, suppressible by Su(dx)^sp/Su(dx)[+]

(Busseau et al., 1994)

dx^ENU has wing phenotype, suppressible by Su(dx)[+]/Su(dx)¹

(Busseau et al., 1994)

Enhancer of

Statement

Reference

dx^ENU/dx[+] is an enhancer of microchaeta | increased number | heat sensitive phenotype of dsh^hs.PA

(Ramain et al., 2001)

Suppressor of

Statement

Reference

dx^ENU/dx[+] is a suppressor of adult thorax & microchaeta phenotype of N^Mcd1

(Ramain et al., 2001)

dx^ENU/dx[+] is a suppressor of adult thorax & microchaeta phenotype of N^Mcd5

(Ramain et al., 2001)

dx^ENU/dx[+] is a suppressor of adult thorax & microchaeta phenotype of N^Mcd8

(Ramain et al., 2001)

Other

Statement

Reference

Delta^9P, Su(dx)[+]/Su(dx)¹, dx^ENU has wing phenotype

(Busseau et al., 1994)

Delta^9P, Su(dx)[+]/Su(dx)¹, dx^ENU has ocellus phenotype

(Busseau et al., 1994)

N^nd-1, Su(dx)¹, dx^ENU has wing phenotype

(Busseau et al., 1994)

Su(dx)[+]/Su(dx)¹, dx^ENU, mam¹⁰/mam[+] has wing phenotype

(Busseau et al., 1994)

Su(dx)[+]/Su(dx)¹, dx^ENU, mam¹⁰/mam[+] has ocellus phenotype

(Busseau et al., 1994)

Delta^9P/Dl[+], Su(dx)[+]/Su(dx)¹, dx^ENU has wing phenotype

(Busseau et al., 1994)

Delta^9P/Dl[+], Su(dx)[+]/Su(dx)¹, dx^ENU has ocellus phenotype

(Busseau et al., 1994)

Su(dx)[+]/Su(dx)¹, dx^ENU, mam¹⁰ has wing phenotype

(Busseau et al., 1994)

Su(dx)[+]/Su(dx)¹, dx^ENU, mam¹⁰ has ocellus phenotype

(Busseau et al., 1994)

Su(dx)¹, dx^ENU, mam¹⁰/mam[+] has wing phenotype

(Busseau et al., 1994)

Su(dx)¹, dx^ENU, mam¹⁰/mam[+] has ocellus phenotype

(Busseau et al., 1994)

Delta^9P/Dl[+], Su(dx)¹, dx^ENU has wing phenotype

(Busseau et al., 1994)

Delta^9P/Dl[+], Su(dx)¹, dx^ENU has ocellus phenotype

(Busseau et al., 1994)

Additional Comments

Genetic Interactions

Statement

Reference

The percentage of N^Ax-9/N^Ax-E2 flies surviving to adulthood is dramatically increased by dx^ENU/+. Survival of N^Ax-9/N^Ax-E2 dx^ENU/+ flies to adulthood is significantly decreased by krz^S047819/+ or krz^S095214/+.

(Mukherjee et al., 2005)

dx^ENU dominantly suppresses the loss of microchaetae seen in N^Mcd1, N^Mcd5 or N^Mcd8 heterozygotes, increasing the number of microchaetae per heminotum to 78.33 +/- 1.09, 94.25 +/- 1.15 or 90.30 +/- 1.61 respectively. dx^ENU dominantly enhances the increase in microchaetae seen when dsh^hs.PA is expressed using heat shock, increasing the number to 142.12 +/- 2.53 thoracic microchaetae per heminotum.

(Ramain et al., 2001)

Triple homozygotes of Su(dx)^sp with N^Ax-E2, dx^ENU are inviable. N^Ax-E2, dx^ENU/N^Ax-9+;Su(dx)¹/+ are inviable, whereas N^nd-1, dx^ENU; Su(dx)¹ are viable though the wing blade is reduced. The mutant wing, eye and ocelli phenotypes are dominantly suppressed by Su(dx)¹, Su(dx)² and Su(dx)^sp. Flies that are dx^ENU/Y; Su(dx)¹/+; mam¹⁰/+ are viable but display clear dx phenotypes in the ocelli and wings. Flies that are dx^ENU/Y; Su(dx)¹/+; Dl^9P/+ are viable but display clear dx phenotypes in the ocelli and wings.

(Busseau et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Xu and Artavanis-Tsakonas.

(Gorman and Girton, 1992)

Comments

Strong dx allele.

(Xu and Artavanis-Tsakonas, 1990)

Variable maternal effect lethality of the dx^ENU chromosome is not linked to dx function.

(Busseau et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dx<up>ENU

(Mukherjee et al., 2005)

dx^ENU

(Sachan et al., 2015, Bejarano et al., 2008)

dx^enu

(Fuwa et al., 2006, Fostier et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close