FlyBase Allele Report: Dmel\N[Mcd8]

General Information

Symbol

Dmel\N^Mcd8

Species

D. melanogaster

Name

Microchaetae defective 8

FlyBase ID

FBal0130885

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Ramain et al., 2001)

Mutagen

X ray

(Ramain et al., 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: S2526term.

(Ramain et al., 2001)

Nucleotide substitution: C16818A.

(Ramain et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:3,170,371..3,170,371 [+]

Nucleotide change:

C3170371A

Reported nucleotide change:

C16818A

Amino acid change:

S2526term | N-PA; S2526term | N-PB

Reported amino acid change:

S2526term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ramain et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | recessive

(Ramain et al., 2001)

some die during pupal stage | recessive

(Ramain et al., 2001)

visible (with N^l1N-ts1)

(Ramain et al., 2001)

visible | dominant

(Ramain et al., 2001)

Phenotype Manifest In

adult thorax & microchaeta

(Ramain et al., 2001)

adult thorax & microchaeta (with N^l1N-ts1)

(Ramain et al., 2001)

embryonic/larval crystal cell | larval stage

(Duvic et al., 2002)

embryonic/larval lymph gland | larval stage

(Duvic et al., 2002)

Detailed Description

Statement

Reference

Increased numbers of crystal cells are seen in N^Mcd8 mutant larvae at late third instar, compared to wild-type. These larvae also have increased numbers of prophenoloxidase expressing cells in the larval lymph glands.

(Duvic et al., 2002)

Heterozygotes show loss of thoracic microchaetae, having 87.58 +/- 1.23 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54). Homozygous adult N^Mcd8 escapers lack 63% of thoracic microchaetae (they have 48.00 +/- 1.66 microchaetae per heminotum), the remaining microchaetae are evenly spaced.. Animals carrying 1 copy of N^Mcd8 in the presence of 2 copies of N⁺ have 93.54 +/- 2.01 thoracic microchaetae per heminotum. Animals carrying 2 copies of N^Mcd8 in the presence of 1 copy of N⁺ have 73.82 +/- 2.02 thoracic microchaetae per heminotum. N^Mcd8/N^l1N-ts1 flies have 91.83 +/- 1.28 thoracic microchaetae per heminotum.

(Ramain et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

N^Mcd8 has visible | recessive phenotype, non-enhanceable by dsh^hs.PA

(Ramain et al., 2001)

Suppressed by

Statement

Reference

N^Mcd8 has visible | dominant phenotype, suppressible by dx^P/dx[+]

(Ramain et al., 2001)

N^Mcd8 has visible | dominant phenotype, suppressible by dx^ENU/dx[+]

(Ramain et al., 2001)

NOT suppressed by

Statement

Reference

N^Mcd8 has visible | recessive phenotype, non-suppressible by dsh^hs.PA

(Ramain et al., 2001)

NOT Suppressor of

Statement

Reference

N^Mcd8 is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

N^Mcd8 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

N^Mcd8 has adult thorax & microchaeta phenotype, non-enhanceable by dsh^hs.PA

(Ramain et al., 2001)

Suppressed by

Statement

Reference

N^Mcd8 has adult thorax & microchaeta phenotype, suppressible by dx^ENU/dx[+]

(Ramain et al., 2001)

N^Mcd8 has adult thorax & microchaeta phenotype, suppressible by dx^P/dx[+]

(Ramain et al., 2001)

NOT suppressed by

Statement

Reference

N^Mcd8 has adult thorax & microchaeta phenotype, non-suppressible by dsh^hs.PA

(Ramain et al., 2001)

NOT Suppressor of

Statement

Reference

N^Mcd8 is a non-suppressor of wing vein phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The thickened wing vein phenotype of adults expressing anchor^GD3613 under the control of Scer\GAL4^A9 is not suppressed by addition of N^Mcd8.

(Wang et al., 2018)

dx^ENU and dx^P dominantly suppress the loss of microchaetae seen in N^Mcd8 heterozygotes, increasing the number of microchaetae per heminotum to 90.30 +/- 1.61 and 98.08 +/- 1.42 respectively. Expression of dsh^hs.PA using a 15 minute 37^oC heat pulse at the beginning of pupariation does not alter the number of thoracic microchaetae produced in N^Mcd8 animals. Su(H)^IB115 dominantly enhances the loss of microchaetae seen in N^Mcd8 heterozygotes, reducing the number of microchaetae per heminotum to 79.67 +/- 1.40.

(Ramain et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

N^Mcd8

(Wang et al., 2018)

Name Synonyms

Microchaetae defective 8

(Ramain et al., 2001)

Secondary FlyBase IDs

References (4)

Report Sections

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