FlyBase Allele Report: Dmel\dsh[hs.PA]

General Information

Symbol

Dmel\dsh^hs.PA

Species

D. melanogaster

Name

heat shock construct of Axelrod

FlyBase ID

FBal0049444

Feature type

allele

Associated gene

Dmel\dsh

Associated Insertion(s)

Carried in Construct

P{Hs:dsh}

Also Known As

hs-dsh

Key Links

Transgenic product class

cDNA

(Axelrod et al., 1996)

wild_type

(Axelrod et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Axelrod et al., 1996)

wild_type

(Axelrod et al., 1996)

Mutagen

in vitro construct

(Axelrod et al., 1998, Axelrod et al., 1996)

Progenitor genotype

Carried in construct

P{Hs:dsh}

Cytology

Description

dsh cDNA is expressed from an Hsp70 promoter.

(Axelrod et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

dsh

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity

(Tree et al., 2002)

abnormal planar polarity | dominant | heat sensitive

(Axelrod et al., 1998)

visible | heat sensitive

(Ramain et al., 2001)

wild-type

(Axelrod et al., 1998)

Phenotype Manifest In

macrochaeta & wing

(Axelrod et al., 1996)

microchaeta | increased number | heat sensitive

(Ramain et al., 2001)

sensory mother cell

(Axelrod et al., 1996)

wing hair

(Tree et al., 2002)

Detailed Description

Statement

Reference

Expression of dsh^hs.PA using a 15 minute 37^oC heat pulse at the beginning of pupariation results in a 5.8% increase in the number of microchaetae.

(Ramain et al., 2001)

Expression of dsh^hs.PA at 24 hours after pupariation results in a dominant planar cell polarity phenotype in the wing. This phenotype is suppressed by dsh^DEP+.hs.

(Axelrod et al., 1998)

Heat induced expression, by application of hot wax, in distal wing regions results in a strong tissue-polarity phenotype, but no reverse polarity is observed.

(Adler et al., 1997)

Pulses of heat shock during late third instar larvae (108-120 hours AEL) result in the development of supernumerary bristles in the interior of the wing, of stout, slender and recurved types, and biased towards the margin. Ectopic SMCs first appear in the late third instar larvae.

(Axelrod et al., 1996)

Overexpression of dsh can inhibit bristle formation, independently of wg.

(Cadigan and Nusse, 1996)

Heat induced expression for 30 minutes between 3 and 4 hours of development causes cardiac hyperplasia, but the hyperplasia is considerably reduced at later stages suggesting the hyperplasia is not a permanent switch to cardiac fate.

(Park et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dsh^hs.PA has visible | heat sensitive phenotype, enhanceable by dx^ENU/dx[+]

(Ramain et al., 2001)

Suppressed by

Statement

Reference

dsh^hs.PA has abnormal cell polarity phenotype, suppressible by pk^pk-sple-13

(Tree et al., 2002)

dsh^hs.PA has abnormal cell polarity phenotype, suppressible by fz²³

(Tree et al., 2002)

NOT Enhancer of

Statement

Reference

dsh^hs.PA is a non-enhancer of visible | recessive phenotype of N^Mcd8

(Ramain et al., 2001)

Suppressor of

Statement

Reference

dsh^hs.PA is a suppressor of visible phenotype of N^nd-3

(Ramain et al., 2001)

dsh^hs.PA is a suppressor of visible phenotype of N^spl-1

(Ramain et al., 2001)

dsh^hs.PA is a suppressor of visible phenotype of N^Ax-9

(Ramain et al., 2001)

dsh^hs.PA is a suppressor of visible phenotype of N^Ax-E2

(Ramain et al., 2001)

NOT Suppressor of

Statement

Reference

dsh^hs.PA is a non-suppressor of abnormal cell polarity phenotype of fz²³

(Tree et al., 2002)

dsh^hs.PA is a non-suppressor of abnormal cell polarity phenotype of pk^pk-sple-13

(Tree et al., 2002)

dsh^hs.PA is a non-suppressor of visible | recessive phenotype of N^Mcd8

(Ramain et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

dsh^hs.PA has microchaeta | increased number | heat sensitive phenotype, enhanceable by dx^ENU/dx[+]

(Ramain et al., 2001)

Suppressed by

Statement

Reference

dsh^hs.PA has wing hair phenotype, suppressible by fz²³

(Tree et al., 2002)

dsh^hs.PA has wing hair phenotype, suppressible by pk^pk-sple-13

(Tree et al., 2002)

Enhancer of

Statement

Reference

dsh^hs.PA is an enhancer of wing hair | heat sensitive phenotype of fz^hs.PP

(Lee and Adler, 2002)

NOT Enhancer of

Statement

Reference

dsh^hs.PA is a non-enhancer of adult thorax & microchaeta phenotype of N^Mcd8

(Ramain et al., 2001)

Suppressor of

Statement

Reference

dsh^hs.PA is a suppressor of microchaeta phenotype of N^nd-3

(Ramain et al., 2001)

dsh^hs.PA is a suppressor of microchaeta phenotype of N^spl-1

(Ramain et al., 2001)

dsh^hs.PA is a suppressor of microchaeta phenotype of N^Ax-9

(Ramain et al., 2001)

dsh^hs.PA is a suppressor of microchaeta phenotype of N^Ax-E2

(Ramain et al., 2001)

dsh^hs.PA is a suppressor of embryonic/larval dorsal vessel phenotype of wg^l-12

(Park et al., 1996)

dsh^hs.PA is a suppressor of embryonic/larval dorsal vessel phenotype of wg^l-17

(Park et al., 1996)

NOT Suppressor of

Statement

Reference

dsh^hs.PA is a non-suppressor of wing hair phenotype of fz²³

(Tree et al., 2002)

dsh^hs.PA is a non-suppressor of wing hair phenotype of pk^pk-sple-13

(Tree et al., 2002)

dsh^hs.PA is a non-suppressor of adult thorax & microchaeta phenotype of N^Mcd8

(Ramain et al., 2001)

Other

Statement

Reference

dsh^hs.PA, wg^hs.PN has wing & macrochaeta phenotype

(Axelrod et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of fz²³ or pk^pk-sple-13 suppresses the wing hair polarity phenotype seen in dsh^hs.PA animals.

(Tree et al., 2002)

Expression of dsh^hs.PA using a 15 minute 37^oC heat pulse at the beginning of pupariation does not alter the number of thoracic microchaetae produced in N^Mcd8 animals. The number of microchaetae in N^nd-3 flies is increased to 119.33 +/- 1.54 by expression of dsh^hs.PA using a heat pulse. The number of microchaetae in N^spl-1 flies is increased to 153.33 +/- 1.42 by expression of dsh^hs.PA using a heat pulse. The number of microchaetae in N^Ax-9 flies is increased to 155.33 +/- 1.89 by expression of dsh^hs.PA using a heat pulse. The number of microchaetae in N^Ax-E2 flies is increased to 126.23 +/- 1.59 by expression of dsh^hs.PA using a heat pulse. dx^ENU dominantly enhances the increase in microchaetae seen when dsh^hs.PA is expressed using heat shock, increasing the number to 142.12 +/- 2.53 thoracic microchaetae per heminotum.

(Ramain et al., 2001)

Induction of wg^hs.PN along with dsh^hs.PA potentiates the response to dsh, causing bristles to form well into the interior of the vast majority of wings. The effect of dsh^hs.PA is potentiated by reduced N or Dl dosage and N^ΔCDC10.hs and suppressed by extra copies of Dl, N or N^int.hs, N^ICN2.hs or N^ΔBam.RV.hs.

(Axelrod et al., 1996)

Heart formation does not occur in dsh^hs.PA, wg^l-12 flies or dsh^hs.PA, wg^l-17 flies reared at 25^oC or 27^oC. Heat shock for 30 minutes between 3 and 4 hours of development rescues heart development, often flies exhibit cardiac hyperplasia.

(Park et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

dsh^hs.PA rescues dsh¹

(Axelrod et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dsh^hs.PA

Name Synonyms

heat shock construct of Axelrod

(Axelrod et al., 1996)

Secondary FlyBase IDs

References (8)

Report Sections

Open Close