FlyBase Allele Report: Dmel\N[Ax-9]

General Information

Symbol

Dmel\N^Ax-9

Species

D. melanogaster

Name

FlyBase ID

FBal0012851

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Also Known As

Ax^9B2, Ax^9B

Key Links

Genomic Maps

JBrowse

Allele class

gain of function allele

antimorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(de Celis and Garcia-Bellido, 1994)

gain of function allele

(Couso and Martinez Arias, 1994)

Mutagen

ethyl methanesulfonate

ethyl nitrosourea

(Xu et al., 1990)

Progenitor genotype

Cytology

Description

Amino acid replacement: D948V. D948 falls in EGF repeat number 24.

(Couso and Martinez Arias, 1994)

Mutation maps to the 24th EGF repeat.

(Diederich et al., 1994)

D to V substitution in the 23rd EGF repeat in the N protein.

(Gorman and Girton, 1992)

Asp⁹⁴⁸ --> Val Mutant partially sequenced; correlated with single amino acid replacements within six adjacent EGF-homologous elements of the N protein; has Gly at residue 2057 characteristic of Oregon R rather than Ser of Canton S (Kelley, Kidd, Deutsch and Young, 1987). Mutation results in an amino acid change from aspartic acid to valine at residue 948 in EGF repeat 24; GAC --> GTC (Kelley, Kidd, Deutsch and Young, 1987).

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:3,165,471..3,165,471 [+]

Nucleotide change:

A3165471T

Reported nucleotide change:

A11919T

Amino acid change:

D948V | N-PA; D948V | N-PB

Reported amino acid change:

D948V

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Kelley et al., 1987)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with N^Ax-E2)

(Xu et al., 1990)

partially lethal (with N^Ax-71d)

(Grushko et al., 1998)

partially lethal (with N^Ax-E2)

(Grushko et al., 1998)

partially lethal - majority die (with N^Ax-E2)

(Mukherjee et al., 2005)

viable

(Grushko et al., 1998)

viable (with N^Ax-1)

(Grushko et al., 1998)

visible

(Ramain et al., 2001)

visible | dominant

(Couso and Martinez Arias, 1994, Gorman and Girton, 1992)

visible | recessive

(Indrasamy et al., 2000)

visible | recessive | temperature conditional

(Indrasamy et al., 2000)

Phenotype Manifest In

adult thoracic sensillum

(Indrasamy et al., 2000)

macrochaeta

(Couso and Martinez Arias, 1994, de Celis and Garcia-Bellido, 1994)

macrochaeta & scutum

(Lyman and Yedvobnick, 1995)

microchaeta

(Ramain et al., 2001)

microchaeta & scutum

(Lyman and Yedvobnick, 1995)

orbital bristle

(Indrasamy et al., 2000)

wing sensillum | temperature conditional

(Indrasamy et al., 2000)

wing vein | temperature conditional

(Indrasamy et al., 2000)

Detailed Description

Statement

Reference

A small percentage of N^Ax-9/N^Ax-E2 flies survive to adulthood.

(Mukherjee et al., 2005)

Mutant flies have 121.55 +/- 1.91 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54).

(Ramain et al., 2001)

Bristle loss and vein interruptions are more extreme at 29[o]C in N^Ax-9.

N^Ax-9 mutants exhibit elevated levels of asymmetry and reduced mean character size relative to Canton-S flies for thoracic bristles.

N^Ax-9 mutants show significantly reduced mean character size for orbital bristles compared to Canton-S flies.

(Indrasamy et al., 2000)

Lethal in combination with N^Ax-16; unescaped female pupae show a strong antineurogenic phenotype.

(Grushko et al., 1998)

Wild type pattern of microchaetae on the head and notum. Heterozygotes with N^l1N-B or N^60g11 exhibit a T-shaped pattern of mechanosensory bristles on the notum, due to bristle loss.

(Lyman and Yedvobnick, 1995)

Lack of bristles.

(Couso and Martinez Arias, 1994)

N^su42c suppresses the pupal lethality of N^Ax-9/N^Ax-E2.

(Diederich et al., 1994)

Su(H)¹⁶, Su(H)^O5 and Su(H)^T4 dominantly suppress the lethality of N^Ax-E2/N^Ax-9 transheterozygotes.

(Fortini and Artavanis-Tsakonas, 1994)

Length of macrochaetae is reduced with respect to that of wild type. Suppresses the N haplo-insufficient phenotype of loss of the wing margin.

(de Celis and Garcia-Bellido, 1994)

Neural precursors never form in the Ax class of N alleles. Cells mutant for Ax class alleles but with some neural potential are inhibited from becoming neural by their neighbours but do not themselves affect their neighbours, which can become neural.

(Heitzler and Simpson, 1993)

Short wing veins II and V. Complex interactions with dx alleles.

(Gorman and Girton, 1992)

Mutant phenotype of transheterozygotes can be rescued when in combination with dx^ENU homozygotes.

(Xu and Artavanis-Tsakonas, 1990)

Viable, suppressor type. N^Ax-E2/N^Ax-9 heterozygotes are lethal.

(Xu et al., 1990)

Viable in both sexes but poorly fertile or sterile. Bristle loss and vein interruptions are more extreme at 29^oC. Heterozygotes of N^Ax-9 with N^Ax-1 and N^Ax-E1 are viable, but N^Ax-9 is inviable with N^Ax-71d, N^Ax-16 and N^Ax-E2 (negative complementation). The lethality associated with negative complementation is suppressed by 23 lethal Notch alleles as well as by alleles of Dl and mam (Xu, Rebay, Fleming, Scottgale and Artavanis-Tsakonas, 1990). When heterozygous with N mutants, phenotypes of Ax and N tend toward normal, but there is temperature sensitivity for suppression of wing nicks (Foster, 1975; Portin, 1975). N^Ax-9 complements every recessive visible on the Notch map at 18^oC to 29^oC (Portin, 1977); with N^Ax-59b and N^Ax-59d, it is semi-lethal. Negative complementation is eliminated by Dp(1;2)51b and results in a strong Ax phenotype (Portin, 1975). The fate map for negatively complementing heteroallelic N^Ax-9/N^Ax-E2 suggests a focus of lethality in tissue close to hypodermal sites of central thoracic structures; in surviving gynandromorphs, negative complementation for morphological defects is autonomous (Portin, 1977b).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^Ax-9/N^Ax-E2, dx^ENU/dx[+] has partially lethal - majority die phenotype, enhanceable by krz^S095214

(Mukherjee et al., 2005)

N^Ax-9/N^Ax-E2 has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2

(Grushko et al., 1998)

N^Ax-9/N^Ax-E2 has partially lethal phenotype, enhanceable by Dp(3;3)bxd¹¹⁰

(Grushko et al., 1998)

N^Ax-9/N^Ax-71d has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2

(Grushko et al., 1998)

N^Ax-9/N^Ax-71d has partially lethal phenotype, enhanceable by Dp(3;3)bxd¹¹⁰

(Grushko et al., 1998)

Suppressed by

Statement

Reference

N^Ax-9/N^Ax-E2 has partially lethal - majority die phenotype, suppressible | partially by dx^ENU/dx[+]

(Mukherjee et al., 2005)

N^Ax-9 has visible phenotype, suppressible by dsh^hs.PA

(Ramain et al., 2001)

N^Ax-9 has visible phenotype, suppressible by A122^A122

(Go and Artavanis-Tsakonas, 1998)

N^Ax-9/N^Ax-E2 has lethal phenotype, suppressible by M285^M285

(Go and Artavanis-Tsakonas, 1998)

N^Ax-9/N^Ax-E2 has lethal phenotype, suppressible by Df(3R)Delta-BX6

(Xu et al., 1990)

N^Ax-9/N^Ax-E2 has lethal phenotype, suppressible by Delta^9P

(Xu et al., 1990)

N^Ax-9/N^Ax-E2 has lethal phenotype, suppressible by mam¹⁰

(Xu et al., 1990)

Suppressor of

Statement

Reference

N^Ax-9/N[+] is a suppressor | partially of visible | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Other

Statement

Reference

Dp(3;3)MKRS-D2, N^Ax-9 has partially lethal phenotype

(Grushko et al., 1998)

N^Ax-9/N^Ax-E2, Su(dx)[+]/Su(dx)¹, dx^ENU has lethal phenotype

(Busseau et al., 1994)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^Ax-9 has microchaeta phenotype, suppressible by dsh^hs.PA

(Ramain et al., 2001)

N^Ax-9 has macrochaeta phenotype, suppressible by A122^A122

(Go and Artavanis-Tsakonas, 1998)

N^Ax-9/N^l1N-B has microchaeta phenotype, suppressible by H¹

(Lyman and Yedvobnick, 1995)

N^Ax-9 has phenotype, suppressible by sno^71e3

(Coyle-Thompson and Banerjee, 1993)

Suppressor of

Statement

Reference

N^Ax-9/N[+] is a suppressor | partially of wing blade posterior compartment | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The wing blistering phenotype seen in the posterior compartment of wings in flies expressing Eogt^GD5084 under the control of Scer\GAL4^en.PU in the presence of Dcr-2^Scer\UAS.cDa is dominantly partially suppressed if the flies are also heterozygous for N^Ax-9.

(Müller et al., 2013)

The percentage of N^Ax-9/N^Ax-E2 flies surviving to adulthood is dramatically increased by dx^ENU/+. Survival of N^Ax-9/N^Ax-E2 dx^ENU/+ flies to adulthood is significantly decreased by krz^S047819/+ or krz^S095214/+.

(Mukherjee et al., 2005)

The number of microchaetae in N^Ax-9 flies is increased to 155.33 +/- 1.89 by expression of dsh^hs.PA using a heat pulse.

(Ramain et al., 2001)

Homozygous viability is decreased if the flies also carry Dp(3;3)MKRS-D2. The antineurogenic phenotype of N^Ax-16/N^Ax-9 female escapers is enhanced in the presence of Dp(3;3)MKRS-D2 or Dp(3;3)bxd¹¹⁰.

(Grushko et al., 1998)

H^hs.PB largely restores microchaetae specification to a wild type pattern in a N^l1N-B/N^Ax-9 background.

(Lyman and Yedvobnick, 1995)

Lethality of N^Ax-E2/N^Ax-9 heterozygotes can be rescued by Df(3R)Dl-BX6, Dl^9P or mam¹⁰.

(Xu et al., 1990)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

N^Ax-E2

(Go and Artavanis-Tsakonas, 1998)

N^Ax-16

(Go and Artavanis-Tsakonas, 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Lefevre.

Comments

N^Ax-9 shows negative complementation with N^Ax-M1.

(de Celis and Garcia-Bellido, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Ax⁹

(Gorman and Girton, 1992)

Ax^9B2-2b

Ax^9B2

(Mukherjee et al., 2005, Indrasamy et al., 2001, Ramain et al., 2001, Indrasamy et al., 2000, Rollins et al., 1999, Go and Artavanis-Tsakonas, 1998, Grushko et al., 1998, Lyman and Yedvobnick, 1995, Busseau et al., 1994, de Celis and Garcia-Bellido, 1994, Diederich et al., 1994, Fortini and Artavanis-Tsakonas, 1994, de Celis et al., 1993, Heitzler and Simpson, 1993, Xu and Artavanis-Tsakonas, 1990, Xu et al., 1990, Grimwade et al., 1985)

Ax^9B

(Wesley, 1999, Verheyen et al., 1996, Couso and Martinez Arias, 1994)

Ax^9b2

(Coyle-Thompson and Banerjee, 1993)

N^Ax-9

N^Ax9B2

(Müller et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (28)

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