FlyBase Allele Report: Dmel\wg[hs.PN]

General Information

Symbol

Dmel\wg^hs.PN

Species

D. melanogaster

Name

heat shock construct of Noordermeer

FlyBase ID

FBal0036008

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{HS-wg}

Also Known As

hs-wg

Key Links

Transgenic product class

wild_type

(Noordermeer et al., 1992)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Noordermeer et al., 1992)

Mutagen

in vitro construct

(Axelrod et al., 1998, Bhat and Schedl, 1997, Boyle et al., 1997, Haerry et al., 1997, Noordermeer et al., 1995, Yanagawa et al., 1995, Thuringer and Bienz, 1993, Noordermeer et al., 1992, Skaer and Martinez Arias, 1992)

Progenitor genotype

Carried in construct

P{HS-wg}

Cytology

Description

wg coding region is expressed under the control of Hsp70 promoter.

(Noordermeer et al., 1992)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal 1 ventral denticle belt

(Noordermeer et al., 1994)

abdominal 2 ventral denticle belt

(Noordermeer et al., 1994)

abdominal 3 ventral denticle belt

(Noordermeer et al., 1994)

abdominal 4 ventral denticle belt

(Noordermeer et al., 1994)

abdominal 5 ventral denticle belt

(Noordermeer et al., 1994)

abdominal 6 ventral denticle belt

(Noordermeer et al., 1994)

abdominal 7 ventral denticle belt

(Noordermeer et al., 1994)

copper cell

(Hoppler and Bienz, 1995)

embryonic/larval dorsal vessel

(Park et al., 1996)

embryonic brain

(Richter et al., 1998)

embryonic epidermis

(Tomlinson et al., 1997)

germarium region 1

(Forbes et al., 1996)

larval RP2 motor neuron

(Bhat, 1996)

larval RP2 motor neuron | ectopic

(Bhat, 1996)

larval ventral nerve cord

(Richter et al., 1998)

mesothoracic pleurum & microchaeta

(Shirras and Couso, 1996)

microchaeta | ectopic

(Shirras and Couso, 1996)

midgut interstitial cell

(Hoppler and Bienz, 1995)

ovariole

(Forbes et al., 1996)

RP2sib neuron | ectopic

(Bhat, 1996)

stalk follicle cell

(Forbes et al., 1996)

sternite

(Shirras and Couso, 1996)

tergite

(Shirras and Couso, 1996)

Detailed Description

Statement

Reference

After heatshocking single Malpighian tubule tip cell always developed appearing morphologically normal.

(Wan et al., 2000)

Overexpression of wg^hs.PN at 24 hours after pupariation produces no phenotype in the wing.

(Axelrod et al., 1998)

Ubiquitous overexpression of wg^hs.PN results in oversized brains and ventral nerve cords. The protocerebrum is increased in size in these brains.

(Richter et al., 1998)

Embryos in which wg^hs.PN is ectopically expressed at 4 hours after egg laying show an increase in the number of somatic gonadal precursor cells.

(Boyle et al., 1997)

Heat induced expression in embryos causes an extreme phenotype, nearly all the epidermal cells secrete smooth cuticle and remnants of denticle belts are observed. An intermediate phenotype: narrow denticle belts due to deletion of the first 3-4 anterior rows of denticles. Weak phenotype, affects only affect the first row of denticles.

(Tomlinson et al., 1997)

Ectopic expression of wg in the neuroectoderm induces duplication of the RP2 lineage in wild type embryos.

(Bhat, 1996)

Ectopic wg expression causes abnormalities in germarium structure, such as elongation of the germarium where region 1 becomes constricted. The number of stalk cells connecting post-germarial egg chambers is reduced and germ-line cysts frequently undergo cell death prior to vitellogenesis resulting in short ovarioles containing fewer than the normal number of egg chambers.

(Forbes et al., 1996)

Overexpression between 3-4 hours of development causes extensive hypertrophy of heart precursor cells. hh⁸, P{HS-wg} embryos kept at 25^oC exhibit lack of heart formation, 30 minutes of heat shock between 3 and 4 hours of development significantly restores heart development.

(Park et al., 1996)

Produced insufficient activity to generate ectopic wing bristles in a wild type background.

(Rulifson et al., 1996)

Ectopic wg expression causes ectopic microchaetae on the pleura and lateral expansion of the tergites and sternites. The time period for each of these effects is 10-22 hours APF for the ectopic bristles and 18-28 hours for expansion of the tergites. Clones found in regions that normally express wg a normal phenotype. Clones found in regions that do not express wg differentiate extra bristles, and expanded tergite and sternite.

(Shirras and Couso, 1996)

Heat shocked embryos do not exhibit copper cells (or a drastic reduction in the number), or interstitial cells but large numbers of large flat cells could be seen. Ubiquitous heat induction after 10 hours of embryonic development causes the 'topping-up' phenotype with large anterior copper cells.

(Hoppler and Bienz, 1995)

The cuticle phenotypes of double mutants of wg^hs.PN with dsh⁴⁷⁷ or arm^25B are indistinguishable from the mutant germline clone phenotypes of dsh, arm and wg embryos, i.e. a lawn of denticles. Double mutants of wg^hs.PN and hh¹¹ show a similar but not identical phenotype to wg^hs.PN alone, i.e. naked throughout most of the ventral cuticle but with dispersed patches of non-polarized denticles evident at the lateral sides. Double mutants of wg^hs.PN and arm^25B show some restoration of the segmental denticle phenotype. The cuticle of double mutant wg^hs.PN, wg^l-17 embryos shows an embryo of almost wild type size with partially restored head and tail structures (filzkorper) and a segmental denticle pattern.

(Noordermeer et al., 1994)

The wg mutant phenotype is at least partly suppressed by three rounds of heat shock activation of wg^hs.PN. Continuous wg activity from 3hrs 40 mins after egg laying rescues the gsb mutant phenotype at least partially.

(Li and Noll, 1993)

Mirror phenotypes result if the wg gene is active during early embryonic development (up to 6 hours) but not later. The result is the same whether the gene product is ubiquitous (wg^hs.PN) or normally localised (wg^l-12).

(Sampedro et al., 1993)

Uniform wg expression generates completely naked cuticle, uniform arm accumulation and broadening of the en domain. Phenotype resembles mutant phenotype of nkd.

(Noordermeer et al., 1992)

Over-expression of wg results in supernumerary cells in the Malpighian tubules.

(Skaer and Martinez Arias, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

wg^hs.PN is a suppressor of abnormal planar polarity | dominant | heat sensitive phenotype of fz^I.hs

(Axelrod et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

wg^hs.PN has larval RP2 motor neuron | ectopic phenotype, suppressible by gsb^hs.PBH

(Bhat, 1996)

wg^hs.PN has RP2sib neuron | ectopic phenotype, suppressible by gsb^hs.PBH

(Bhat, 1996)

Suppressor of

Statement

Reference

wg^hs.PN is a suppressor of wing phenotype of fz^I.hs

(Axelrod et al., 1998)

Other

Statement

Reference

en^E, inv^E, wg^hs.PN has larval RP2 motor neuron | ectopic phenotype

(Bhat and Schedl, 1997)

dsh^hs.PA, wg^hs.PN has wing & macrochaeta phenotype

(Axelrod et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Suppresses the dominant gain-of-function planar cell polarity phenotype in the wing produced by overexpression of fz^I.hs at 24 hours after pupariation.

(Axelrod et al., 1998)

en^E inv^E embryos expressing wg^hs.PN have triplicated RP2 neurons.

(Bhat and Schedl, 1997)

Induction of wg^hs.PN along with dsh^hs.PA potentiates the response to dsh, causing bristles to form well into the interior of the vast majority of wings.

(Axelrod et al., 1996)

Ectopic expression of gsb^hs.PBH in the neuroectoderm (in a wg^hs.PN background) suppresses the RP2 lineage duplication. Ectopic expression in Df(2R)gsb embryos causes triplication of the RP2 lineage.

(Bhat, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Noordermeer.

(Sampedro et al., 1993, Thuringer and Bienz, 1993, Skaer and Martinez Arias, 1992)

Comments

Displays a variety of wg-dependent effects that are mediated by dsh (FBrf0055887).

(Yanagawa et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

wg^hs.PN

Name Synonyms

heat shock construct of Noordermeer

Secondary FlyBase IDs

References (40)

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