FlyBase Allele Report: Dmel\gsb[hs.PBH]

General Information

Symbol

Dmel\gsb^hs.PBH

Species

D. melanogaster

Name

heat shock construct of Basler and Hafen

FlyBase ID

FBal0034748

Feature type

allele

Associated gene

Dmel\gsb

Associated Insertion(s)

Carried in Construct

P{Hsgsb.BH}

Also Known As

gsb-d^hs.PBH, Hs-gsb

Key Links

Transgenic product class

cDNA

(Li and Noll, 1993)

wild_type

(Li and Noll, 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Li and Noll, 1993)

wild_type

(Li and Noll, 1993)

Mutagen

in vitro construct

(Li and Noll, 1994, Li and Noll, 1993)

Progenitor genotype

Carried in construct

P{Hsgsb.BH}

Cytology

Description

gsb expression (cDNA BSH9c2) is driven by an Hsp70 promoter.

(Li and Noll, 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

gsb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | heat sensitive

(Li and Noll, 1993)

pair rule phenotype | embryonic stage

(Li and Noll, 1994)

some die during embryonic stage | heat sensitive

(Li and Noll, 1993)

Phenotype Manifest In

abdominal 1 ventral denticle belt | heat sensitive

(Li and Noll, 1993)

abdominal 2 ventral denticle belt | embryonic stage

(Li and Noll, 1994)

abdominal 2 ventral denticle belt | heat sensitive

(Li and Noll, 1993)

abdominal 3 ventral denticle belt | heat sensitive

(Li and Noll, 1993)

abdominal 4 ventral denticle belt | embryonic stage

(Li and Noll, 1994)

abdominal 4 ventral denticle belt | heat sensitive

(Li and Noll, 1993)

abdominal 5 ventral denticle belt | heat sensitive

(Li and Noll, 1993)

abdominal 6 ventral denticle belt | embryonic stage

(Li and Noll, 1994)

abdominal 6 ventral denticle belt | heat sensitive

(Li and Noll, 1993)

abdominal 7 ventral denticle belt | heat sensitive

(Li and Noll, 1993)

abdominal 8 ventral denticle belt | embryonic stage

(Li and Noll, 1994)

larval EL neuron

(Li and Noll, 1994)

larval RP2 motor neuron

(Bhat et al., 2000, Li and Noll, 1994)

RP2sib neuron

(Bhat et al., 2000)

U neuron

(Li and Noll, 1994)

Detailed Description

Statement

Reference

Heat shock induction after 2-3 hours of development causes a pair rule cuticular phenotype, even numbered abdominal denticle belts and anteriorly adjacent naked regions are deleted. Later heat induction after 3-6 hours of development produces a naked cuticular phenotype, loss of RP2 and EL neurons and increase in CQ neurons.

(Li and Noll, 1994)

Ubiquitous expression of gsb results in the loss of denticle belts in most embryos subject to a heat shock between 3hrs 10 mins and 6 hrs 20 mins after egg laying. After this time heat shock has no effect.

(Li and Noll, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

gsb^hs.PBH is a suppressor of larval RP2 motor neuron | ectopic phenotype of wg^hs.PN

(Bhat, 1996)

gsb^hs.PBH is a suppressor of RP2sib neuron | ectopic phenotype of wg^hs.PN

(Bhat, 1996)

Other

Statement

Reference

gsb^hs.PBH, slp1^hs.PC has larval RP2 motor neuron phenotype

(Bhat et al., 2000)

gsb^hs.PBH, slp1^hs.PC has RP2sib neuron phenotype

(Bhat et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The RP2/sib lineage is missing in 97% of hemisegments in embryos carrying two copies of gsb^hs.PBH and one copy of slp1^hs.PC, as is seen in embryos carrying only gsb^hs.PBH. In contrast, in embryos containing either one copy of gsb^hs.PBH and one copy of slp1^hs.PC or two copies of gsb^hs.PBH and two copies of slp1^hs.PC, both loss and gain of the RP2/sib lineage is seen in a partially penetrant manner.

(Bhat et al., 2000)

gsb^hs.PBH prevents the duplication of RP2 neurons seen in en^E inv^E embryos when expressed during stage 8.

(Bhat and Schedl, 1997)

Ectopic expression of gsb, P{Hsgsb.BH}, in the neuroectoderm suppresses the RP2 lineage duplication induced by ectopic expression of wg, P{HS-wg}.

(Bhat, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gsb-d^hs.PBH

(Jagla et al., 1997, Bhat, 1996, Li and Noll, 1993)

gsb^hs.PBH

Name Synonyms

heat shock construct of Basler and Hafen

Secondary FlyBase IDs

References (6)

Report Sections

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