FlyBase Allele Report: Dmel\N[Mcd5]

General Information

Symbol

Dmel\N^Mcd5

Species

D. melanogaster

Name

Microchaetae defective 5

FlyBase ID

FBal0130887

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Ramain et al., 2001)

Mutagen

ethyl methanesulfonate

(Ramain et al., 2001)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G11292A. Amino acid replacement: C739Y.

(Ramain et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:3,164,844..3,164,844 [+]

Nucleotide change:

G3164844A

Reported nucleotide change:

G11292A

Amino acid change:

C739Y | N-PA; C739Y | N-PB

Reported amino acid change:

C739Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ramain et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage | recessive

(Ramain et al., 2001)

some die during larval stage | recessive

(Ramain et al., 2001)

visible (with N^l1N-ts1)

(Ramain et al., 2001)

visible | dominant

(Ramain et al., 2001)

visible | recessive | somatic clone

(Ramain et al., 2001)

Phenotype Manifest In

adult thorax & microchaeta

(Ramain et al., 2001)

adult thorax & microchaeta (with N^l1N-ts1)

(Ramain et al., 2001)

adult thorax & microchaeta | somatic clone

(Ramain et al., 2001)

Detailed Description

Statement

Reference

Heterozygotes show loss of thoracic microchaetae, having 89.25 +/- 1.90 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54). 99% of thoracic microchaetae are missing in homozygous clones, whereas macrochaetae are unaffected. Animals carrying 1 copy of N^Mcd5 in the presence of 2 copies of N⁺ have 114.83 +/- 2.58 thoracic microchaetae per heminotum. Animals carrying 2 copies of N^Mcd5 in the presence of 1 copy of N⁺ have 72.92 +/- 2.44 thoracic microchaetae per heminotum. N^Mcd5/N^l1N-ts1 flies have 36.75 +/- 1.88 thoracic microchaetae per heminotum. Homozygous pupae show loss of microchaetae precursors in the thorax.

(Ramain et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^Mcd5 has visible | dominant phenotype, enhanceable by Su(H)[+]/Su(H)^IB115

(Ramain et al., 2001)

Suppressed by

Statement

Reference

N^Mcd5 has visible | dominant phenotype, suppressible by dx^ENU/dx[+]

(Ramain et al., 2001)

N^Mcd5 has visible | dominant phenotype, suppressible by dx^P/dx[+]

(Ramain et al., 2001)

Other

Statement

Reference

Delta^RevF10, N^Mcd5, Ser^RX82 has visible | somatic clone phenotype

(Ramain et al., 2001)

N^Mcd5, Su(H)^IB115 has visible | somatic clone phenotype

(Ramain et al., 2001)

Delta^unspecified, N^Mcd5 has visible | somatic clone phenotype

(Ramain et al., 2001)

Df(3R)gro^b32.2, N^Mcd5 has visible | somatic clone phenotype

(Ramain et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^Mcd5 has adult thorax & microchaeta phenotype, enhanceable by Su(H)[+]/Su(H)^IB115

(Ramain et al., 2001)

Suppressed by

Statement

Reference

N^Mcd5 has adult thorax & microchaeta phenotype, suppressible by dx^ENU/dx[+]

(Ramain et al., 2001)

N^Mcd5 has adult thorax & microchaeta phenotype, suppressible by dx^P/dx[+]

(Ramain et al., 2001)

Other

Statement

Reference

Delta^RevF10, N^Mcd5, Ser^RX82 has microchaeta | somatic clone phenotype

(Ramain et al., 2001)

N^Mcd5, Su(H)^IB115 has microchaeta | somatic clone phenotype

(Ramain et al., 2001)

Delta^unspecified, N^Mcd5 has microchaeta | somatic clone phenotype

(Ramain et al., 2001)

Df(3R)gro^b32.2, N^Mcd5 has microchaeta | somatic clone phenotype

(Ramain et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

N^Mcd5 Dl^unspecified double mutant clones do not develop microchaetae but produce epidermis. N^Mcd5 Dl^RevF10 Ser^RX82 triple mutant clones do not develop microchaetae but produce epidermis. N^Mcd5 Su(H)^IB115 double mutant clones do not develop microchaetae but produce epidermis. Df(3R)gro^b32.2 double mutant clones do not develop microchaetae but produce epidermis. Macrochaetae in N^Mcd5 Dl^RevF10 double mutant clones develop as single bristles rather than as a neurogenic tuft. dx^ENU and dx^P dominantly suppress the loss of microchaetae seen in N^Mcd5 heterozygotes, increasing the number of microchaetae per heminotum to 94.25 +/- 1.15 and 105.33 +/- 3.08 respectively. Su(H)^IB115 dominantly enhances the loss of microchaetae seen in N^Mcd5 heterozygotes, reducing the number of microchaetae per heminotum to 69.92 +/- 1.86.

(Ramain et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

N^Mcd5

Name Synonyms

Microchaetae defective 5

(Ramain et al., 2001)

Secondary FlyBase IDs

References (1)

Report Sections

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