FlyBase Aberration Report: Dmel\Df(3R)gro[b32.2]

FB2024_04 , released June 25, 2024

FB2024_04 , released June 25, 2024

Aberration: Dmel\Df(3R)gro^b32.2

General Information

Symbol

Df(3R)gro^b32.2

Species

D. melanogaster

Name

FlyBase ID

FBab0022421

Feature type

chromosomal_deletion

Also Known As

Df(3R)E(spl)^b32.2, E(spl)^b32.2, Df(3R)E(spl)^grob32.2, Df(E(spl)), E(spl)gro^b32.2, Df(3R)gro^32.2

Computed Breakpoints include

[96F9];[96F11]

Features within 96F9--96F11

Sequence coordinates

Member of large scale dataset(s)

Nature of Aberration

Cytological Order

Progenitor

(Schrons et al., 1992)

Mutagen

Delta2-3 transposase

(Schrons et al., 1992)

Class of aberration (relative to wild type)

chromosomal_deletion

(FlyBase, 2007)

Class of aberration (relative to progenitor)

chromosomal_deletion

(Schrons et al., 1992)

Breakpoints

Causes alleles

(de Celis et al., 1996)

Carries alleles

Transposon Insertions

Formalized genetic data

boss << bk1 << HLHmδ << bk2 hits gro

Genetic mapping information

Comments

Comments on Cytology

Left limit of break 1 from non-inclusion of boss (FBrf0056226) Right limit of break 1 from inclusion of HLHmβ (FBrf0085207) Left limit of break 2 from inclusion of E(spl) (FBrf0056226) Right limit of break 2 from non-inclusion of gro (FBrf0056226)

Sequence Crossreferences

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Gene Deletion and Duplication Data

Genes Deleted / Disrupted

Complementation Data

Completely deleted / disrupted

(Ligoxygakis et al., 1998, de Celis et al., 1997, de Celis et al., 1996, Heitzler et al., 1996)

(de Celis et al., 1996)

(Ligoxygakis et al., 1998, de Celis et al., 1997, de Celis et al., 1996, Heitzler et al., 1996)

(Ligoxygakis et al., 1998, de Celis et al., 1997, de Celis et al., 1996, Heitzler et al., 1996)

(Ligoxygakis et al., 1998, de Celis et al., 1996, Schrons et al., 1992)

(Ligoxygakis et al., 1998, de Celis et al., 1997, de Celis et al., 1996, Heitzler et al., 1996)

(Ligoxygakis et al., 1998, de Celis et al., 1997, de Celis et al., 1996, Heitzler et al., 1996)

(Ligoxygakis et al., 1998, de Celis et al., 1997, de Celis et al., 1996, Heitzler et al., 1996)

(de Celis et al., 1997, Heitzler et al., 1996)

Partially deleted / disrupted

(Ligoxygakis et al., 1998, de Celis et al., 1996, Schrons et al., 1992)

Molecular Data

Completely deleted

Partially deleted

Genes NOT Deleted / Disrupted

Complementation Data

(Schrons et al., 1992)

Molecular Data

Genes Duplicated

Complementation Data

Completely duplicated

Partially duplicated

Molecular Data

Completely duplicated

Partially duplicated

Genes NOT Duplicated

Complementation Data

Molecular Data

Affected Genes Inferred by Location

Related Comments

Df(3R)gro^b32.2 does not delete gro but it is nevertheless affected.

(Ligoxygakis et al., 1998)

Phenotypic Data

In combination with other aberrations

Df(3R)ED6232/Df(3R)gro^b32.2, in combination with gro^+t10.4, mutant embryos do not display any significant change in the number of Ap neurons, as compared with controls.

(Bivik et al., 2016)

Df(3R)gro^b32.2/Df(3R)Exel6204 or Df(3R)E(spl)δ-6/Df(3R)gro^b32.2, in combination with gro^+t10.4, mutant embryos display a significant increase in the number of Ap neurons, as compared with controls.

(Bivik et al., 2016)

NOT in combination with other aberrations

Df(3R)gro^b32.2 ; gro^+t10.4 intestinal stem cell (ISC) clones show an overproliferation of small ISC-like cells.

(Bardin et al., 2010)

Wound closure does not appear to be compromised compared to controls in Df(3R)gro^b32.2/+ wing discs which have been fragmented (a 90[o] sector has been dissected out of the posterior compartment, leaving a 3/4 anterior fragment) and then implanted into wild-type females (analysed at 24 and 48 hours after implantation). However, the Df(3R)gro^b32.2/+ regenerating discs show significantly impaired proliferation compared to controls.

(Blanco et al., 2010)

Homozygous Df(3R)gro^b32.2 embryos display a neurogenic phenotype.

(Monastirioti et al., 2010)

Df(3R)gro^b32.2 follicle cells clones carrying gro^+t10.4 do not undergo extra cell divisions compared to wild type.

(Deng et al., 2001)

Somatic clones in the follical cells, do not have any detectable phenotype.

(Lopez-Schier and St. Johnston, 2001)

Homozygous clones develop densely packed microchaetae within the clone.

(Ramain et al., 2001)

Df(3R)gro^b32.2 gro^+t10.4 clones in the eye disc show a cell autonomous neurogenic phenotype.

(Ligoxygakis et al., 1998)

Homozygous clones in the leg form normal joints even when they span more than one segment and show differentiation of ectopic sensory organs.

(de Celis et al., 1998)

Scer\GAL4^da.G32-mediated coexpression of l(1)sc^UAS.cHa and da^UAS.cGa cause increase of cells in the CNS: brain lobes are enlarged (protruding through the holes in the cephalic epidermis), the ventral cord shows regional enlargement and sensory organs contain a large number of neurons. Neural hyperplasia is also increased, epidermis, fore- and hindgut, tracheal tree and salivary glands are completely neuralised.

(Giebel et al., 1997)

Ventral mitotic clone in the wing causes thickening of the central component of wing vein LIII but does not affect vein differentiation in the opposite surface. Clones induced in a rho^ve-1 vn¹ mutant background that cover the vein LV territory do not differentiate a vein.

(de Celis et al., 1997)

Df(3R)gro^b32.2 gro^+t10.4 clones differentiate dense clumps of microchaetes that are frequently seen to be adjacent to one another. A small amount of epidermis differentiates and there are areas where bristles are intermingled with epidermal hairs. Small tufts of macrochaetes can be seen.

(Heitzler et al., 1996)

Expression of E(spl)m8-HLH^UAS.cNa under the influence of Scer\GAL4^da.G32 attenuates the mutant phenotype of Df(3R)gro^b32.2. A weaker attenuation is seen with E(spl)m5-HLH^UAS.cNa. Simultaneous expression of E(spl)m8-HLH^UAS.cNa or E(spl)m5-HLH^UAS.cNa with E(spl)m8-HLH^1,bd-.UAS driven by Scer\GAL4^da.G32 causes severe neural hypoplasia in embryos and adults, and reduces the level of neural hyperplasia in Df(3R)gro^b32.2.

(Nakao and Campos-Ortega, 1996)

Complements the gro¹ allele. Clones in the thorax generate dense clusters of bristles at the locations that the macrochaetae develop. The microchaetae differentiate at high density forming large fields of bristles. The phenotype suggests that the E(spl) complex genes are required for segregation of the SOPs but not for their further differentiation. Duplicated trichogens occasionally occur in these clones, associated with fused tormogens, due to close packing of SOPs, rather than changes in SOP progeny identity. Clones in the wing that reach the wing margin show a loss of sensory structures along the wing margin. Wing veins also appear thicker.

(de Celis et al., 1996)

In a gro^+t9.2 background, Df(3R)gro^b32.2/+ or Df(3R)gro^b32.2/Df(3R)E(spl)-r72.1 strongly suppresses the bristle loss phenotype of H²⁰/H^E31.

(Bang et al., 1995)

Stocks (1)

52011

P{neoFRT}82B Df(3R)gro^b32.2 P{E(spl)E8}3/TM6B, Tb¹

Notes on Origin

Discoverer

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

Df(3)32.2

(Couturier et al., 2019)

Df(3R) E(spl) b32.2

(Chen et al., 2018)

Df(3R)(spl)^b32.2

(Koelzer and Klein, 2003)

Df(3R)E(spl)^R-b32.2

(Giebel et al., 1997)

Df(3R)E(spl)<up>b32.2

(Monastirioti et al., 2010)

Df(3R)E(spl)^b32.2

(Majot and Bidwai, 2017, Zacharioudaki et al., 2016, Wang et al., 2011, Tapanes-Castillo and Baylies, 2004, Ramain et al., 2001)

Df(3R)E(spl)^grob32.2

(Cooper and Bray, 1999, de Celis et al., 1998)

Df(3R)E(spl)[b32.2]

(Babaoğlan et al., 2013)

Df(3R)E(spl)b32.2

(Janssens et al., 2017)

Df(3R)b^32.2

(Lecourtois and Schweisguth, 1998)

Df(3R)gro32.2

(Bivik et al., 2016)

Df(3R)gro^32.2

(Guo et al., 2019, Bardin et al., 2010)

Df(3R)gro^b32.2

(Blanco et al., 2010)

Df(3R)gro^ry78R-b32.2

(Schrons et al., 1992)

Df(E(spl))

(Liu et al., 2017, Blanco et al., 2010)

E(spl)

(Monastirioti et al., 2010)

E(spl)32.2

(Couturier et al., 2019)

E(spl)^b32.2

(Venken, 2006, Pi et al., 2001, Apidianakis et al., 1999, de Celis et al., 1997, de Celis et al., 1996, Bang et al., 1995)

E(spl)gro^b32.2

(Bhattacharya et al., 2017, Bhattacharya and Baker, 2009)

gro^b32.2

(Lopez-Schier and St. Johnston, 2001)

Name Synonyms

Secondary FlyBase IDs

References (40)

Report Sections