FB2024_03 , released June 25, 2024
Allele: Dmel\E(spl)m5-HLHUAS.cNa
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General Information
Symbol
Dmel\E(spl)m5-HLHUAS.cNa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Nakao
FlyBase ID
FBal0052334
Feature type
allele
Associated gene
Dmel\E(spl)m5-HLH
Associated Insertion(s)
Carried in Construct
P{UAS-E(spl)m5-HLH.N}
Key Links
Transgenic product class
wild_type
(Nakao and Campos-Ortega, 1996)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Nakao and Campos-Ortega, 1996)
Mutagen
in vitro construct
(Nakao and Campos-Ortega, 1996)
Progenitor genotype
Carried in construct
P{UAS-E(spl)m5-HLH.N}
Cytology
Description

UASt regulatory sequences drive expression of the E(spl)m5-HLH coding region.

(Nakao and Campos-Ortega, 1996)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
E(spl)m5-HLH
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When expressed under the influence of Scer\GAL4da.G32, HLHm5Scer\UAS.cNa can cause central and peripheral neural hypoplasia. There are no obvious epidermal effects on these embryos. The severity of this phenotype depends on the site of insertion of the P{HLHm5-UAST} element. This effect is dramatically enhanced if the flies also carry P{UAS-E(spl).N}. When expressed under the influence of Scer\GAL4sca-537.4, HLHm5Scer\UAS.cNa can cause defects in the patterns of bristles. Pupal lethal lines from Scer\GAL4da.G32 or Scer\GAL4sca-537.4 lack almost all macro and microchaetae. Wing margin bristles are particularly affected in HLHm5Scer\UAS.cNa.

      (Nakao and Campos-Ortega, 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression under the influence of Scer\GAL4da.G32 attenuates the mutant phenotype of Df(3R)grob32.2, though to a lesser degree than for E(spl)Scer\UAS.cNa. Simultaneous expression of E(spl)Scer\UAS.cNa or HLHm5Scer\UAS.cNa with E(spl)1,bd-.Scer\UAS driven by Scer\GAL4da.G32 causes severe neural hypoplasia in embryos and adults, and reduces the level of neural hyperplasia in Df(3R)grob32.2.

      (Nakao and Campos-Ortega, 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      E(spl)m5-HLHScer\UAS.cNa
      E(spl)m5-HLHUAS.cNa
      HLHm5Scer\UAS.cNa
      HLHm5UAS.cNa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Nakao
      (Nakao and Campos-Ortega, 1996)
      Secondary FlyBase IDs
        References (3)