FlyBase Allele Report: Dmel\N[Mcd1]

General Information

Symbol

Dmel\N^Mcd1

Species

D. melanogaster

Name

Microchaetae defective 1

FlyBase ID

FBal0130890

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Ramain et al., 2001)

Mutagen

ethyl methanesulfonate

(Ramain et al., 2001)

Progenitor genotype

Cytology

Description

AAG (starting at nucleotide position 15643) is replaced with C. This results in a frameshift after amino acid residue G2189, producing a truncated protein containing 2189 N residues followed by an additional 138 amino acid residues.

(Ramain et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Ramain et al., 2001)

visible (with N^l1N-ts1)

(Ramain et al., 2001)

visible | dominant

(Escudero et al., 2003, Ramain et al., 2001)

visible | recessive | somatic clone

(Ramain et al., 2001)

Phenotype Manifest In

adult midgut

(de Navascués et al., 2012)

adult thorax & microchaeta

(Ramain et al., 2001)

adult thorax & microchaeta (with N^l1N-ts1)

(Ramain et al., 2001)

adult thorax & microchaeta | somatic clone

(Ramain et al., 2001)

enteroblast of posterior adult midgut epithelium

(de Navascués et al., 2012)

intestinal stem cell of posterior adult midgut epithelium

(de Navascués et al., 2012)

microchaeta

(Escudero et al., 2003)

Detailed Description

Statement

Reference

Midguts from N^Mcd1 heterozygotes at 10 days of age show abnormal intestinal stem cell to enteroblast ratio, imbalanced towards the latter.

(de Navascués et al., 2012)

Heterozygotes have a low density of microchaetae on the notum.

(Escudero et al., 2003)

Heterozygotes show loss of thoracic microchaetae, having 69.83 +/- 1.11 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54). 99% of thoracic microchaetae are missing in homozygous clones, whereas macrochaetae are unaffected. Animals carrying 1 copy of N^Mcd1 in the presence of 2 copies of N⁺ have 90.67 +/- 1.39 thoracic microchaetae per heminotum. Animals carrying 2 copies of N^Mcd1 in the presence of 1 copy of N⁺ have 40.33 +/- 1.31 thoracic microchaetae per heminotum. N^Mcd1/N^l1N-ts1 flies have 53.83 +/- 1.78 thoracic microchaetae per heminotum.

(Ramain et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^Mcd1 has visible | dominant phenotype, enhanceable by Su(H)[+]/Su(H)^IB115

(Ramain et al., 2001)

Suppressed by

Statement

Reference

N^Mcd1 has visible | dominant phenotype, suppressible by dx^P/dx[+]

(Ramain et al., 2001)

N^Mcd1 has visible | recessive | somatic clone phenotype, suppressible by sgg³²

(Ramain et al., 2001)

N^Mcd1 has visible | dominant phenotype, suppressible by dx^ENU/dx[+]

(Ramain et al., 2001)

NOT suppressed by

Statement

Reference

N^Mcd1 has visible | dominant phenotype, non-suppressible by ed^slH8/ed^1X5

(Escudero et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^Mcd1 has microchaeta & mesothoracic tergum phenotype, enhanceable by Hip1^UAS.cMa/Scer\GAL4^pnr-MD237

(Moores et al., 2008)

N^Mcd1 has adult thorax & microchaeta phenotype, enhanceable by Su(H)[+]/Su(H)^IB115

(Ramain et al., 2001)

Suppressed by

Statement

Reference

N^Mcd1 has microchaeta & mesothoracic tergum phenotype, suppressible by Scer\GAL4^pnr-MD237/Hip1^ΔANTH.UAS

(Moores et al., 2008)

N^Mcd1 has adult thorax & microchaeta phenotype, suppressible by sgg³²

(Ramain et al., 2001)

N^Mcd1 has adult thorax & microchaeta phenotype, suppressible by dx^ENU/dx[+]

(Ramain et al., 2001)

N^Mcd1 has adult thorax & microchaeta phenotype, suppressible by dx^P/dx[+]

(Ramain et al., 2001)

NOT suppressed by

Statement

Reference

N^Mcd1 has microchaeta phenotype, non-suppressible by ed^slH8/ed^1X5

(Escudero et al., 2003)

Suppressor of

Statement

Reference

N^Mcd1 is a suppressor of microchaeta | ectopic | somatic clone phenotype of gro^E48

(Ramain et al., 2001)

NOT Suppressor of

Statement

Reference

N^Mcd1 is a non-suppressor of macrochaeta | ectopic | somatic clone phenotype of emc¹

(Ramain et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Hip1^{ΔANTH.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 suppresses the abnormal microchaetae density phenotype seen on the notum of N^Mcd1 flies.

Expression of Hip1^Scer\UAS.cMa under the control of Scer\GAL4^pnr-MD237 enhances the abnormal microchaetae density phenotype seen on the notum of N^Mcd1 flies.

(Moores et al., 2008)

The low density of microchaetae on the notum seen in N^Mcd1/+ flies is not rescued by ed^1X5/ed^slH8.

(Escudero et al., 2003)

N^Mcd1 gro^E48 double mutant clones lack the ectopic microchaetae seen in gro^E48 clones. Ectopic macrochaetae are seen in N^Mcd1 emc¹ double mutant clones (as is seen in emc¹ single mutant clones). dx^ENU and dx^P dominantly suppress the loss of microchaetae seen in N^Mcd1 heterozygotes, increasing the number of microchaetae per heminotum to 78.33 +/- 1.09 and 76.42 +/- 2.21 respectively. Su(H)^IB115 dominantly enhances the loss of microchaetae seen in N^Mcd1 heterozygotes, reducing the number of microchaetae per heminotum to...

(Ramain et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

N^MCD1

(de Navascués et al., 2012, Moores et al., 2008)

N^Mcd1

N^mcd1

(Escudero et al., 2003)

Name Synonyms

Microchaetae defective 1

(Ramain et al., 2001)

Secondary FlyBase IDs

References (4)

Report Sections

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