FlyBase Allele Report: Dmel\sgg[32]

General Information

Symbol

Dmel\sgg³²

Species

D. melanogaster

Name

FlyBase ID

FBal0015510

Feature type

allele

Associated gene

Dmel\sgg

Associated Insertion(s)

Carried in Construct

Also Known As

sgg^D127, zw3^D127, zw3^sggD127

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Cadigan and Nusse, 1996, Miyamoto et al., 1995, Ruel et al., 1993)

loss of function allele

(Lecuit and Cohen, 1997)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

1st axillary sclerite | somatic clone

(Buratovich and Wilder, 2001)

2nd axillary sclerite | somatic clone

(Buratovich and Wilder, 2001)

3rd axillary sclerite | ectopic | somatic clone

(Buratovich and Wilder, 2001)

adult cuticle & abdomen | somatic clone

(Lawrence et al., 2002)

adult thorax & macrochaeta | somatic clone

(Simpson and Carteret, 1990)

adult thorax & microchaeta | somatic clone

(Simpson and Carteret, 1990)

axillary pouch | somatic clone

(Buratovich and Wilder, 2001)

cuticle | ectopic | somatic clone

(Royet and Finkelstein, 1997)

leg

(Diaz-Benjumea and Cohen, 1994)

macrochaeta

(Diaz-Benjumea and Cohen, 1994)

macrochaeta & mesothoracic tergum | somatic clone | cell autonomous

(Phillips et al., 1999)

macrochaeta & prescutum | somatic clone | cell autonomous

(Phillips et al., 1999)

macrochaeta & scutum | somatic clone | cell autonomous

(Phillips et al., 1999)

macrochaeta & wing | ectopic | somatic clone

(Schweizer et al., 2003)

macrochaeta | cell autonomous | ectopic | somatic clone

(Phillips et al., 1999)

macrochaeta | ectopic | somatic clone

(Lawrence et al., 2002)

macrochaeta | somatic clone | increased number

(Kanuka et al., 2005)

microchaeta | ectopic | somatic clone

(Lawrence et al., 2002)

microchaeta | somatic clone | increased number

(Kanuka et al., 2005)

ommatidium | somatic clone

(Royet and Finkelstein, 1997)

unnamed wing hinge sclerite | somatic clone

(Buratovich and Wilder, 2001)

wing | anterior | somatic clone

(Jia et al., 2002)

Detailed Description

Statement

Reference

29.2% of eggs derived from mothers carrying sgg³² mutant germline clones are able to complete meiosis. The remaining 70.8% exhibit abnormal meiotic defects.

(Takeo et al., 2012)

sgg³² mitotic clones result in extra macrochaetae and microchaetae in the dorsal part and scutellum of the adult notum, which corresponds to the wg-expressing region.

(Kanuka et al., 2005)

Homozygous mutant clones give rise to ectopic bristles on the adult wing.

(Schweizer et al., 2003)

Homozygous clones in the anterior of the wing form small outgrowths.

(Jia et al., 2002)

sgg³² somatic clones in tergites are abnormally round in shape and have higher than normal bristle densities. In addition polarity reversals are seen; hairs and bristles at the back of clones are reversed. sgg³² somatic clones in the adult abdomen can transform segmental region anterior 1 (a1) cuticle into segmental region anterior 3 (a3) cuticle. Such clones in the segmental region posterior 2 (p2) can sometimes become hairy, suggesting a transformation to region p3.

(Lawrence et al., 2002)

Large homozygous clones that cover most of the dorsal wing hinge cause duplication of axillary sclerite 3 at the expense of axillary sclerite 1, axillary sclerite 2 and the unnamed plate. Clones in the ventral wing hinge cause reduction or loss of the axillary pouch, depending on the size of the clone.

(Buratovich and Wilder, 2001)

Homozygous sgg³² or sgg³²/sgg^M11 adults (that have been rescued to adulthood by the addition of sgg^hs.P coupled with larval and pupal heatshocks), show a lengthening of the locomotor activity rhythm periodicity to about 26 hours.

(Martinek et al., 2001)

Homozygous clones autonomously form extra macrochaetae in the notum. These appear most frequently near the dorsocentral meridian and in the scutellum and less frequently near the lateral macrochaetae. Extra macrochaetae are also seen medially in the scutum and prescutum, far from the normal site of any wild-type macrochaetae. The macrochaetae are confined to the posterior region of the clone, irrespective of the location of the clone in the notum.

(Phillips et al., 1999)

sgg³² Df(1)sc-B57 clones in the wing show no disturbance in planar polarity.

(Axelrod et al., 1998)

Homozygous clones in the eye cause replacement of ommatidia by ectopic cuticle, which shows the typical ridged morphology of frons cuticle.

(Royet and Finkelstein, 1997)

sgg³² mutant cells within clones present in the eye fail to differentiate and progression of the morphogenetic furrow is blocked. Wild type cells anterior to the clone fail to express neuronal markers confirming that the morphogenetic furrow is unable to reinitiate beyond a block caused by the wg pathway.

(Treisman and Rubin, 1995)

Clones of mutant cells induced in the leg show that removal of sgg activity is sufficient to specify ventral cell fate. Such clones can reorganise the dorsal-ventral axis of the leg. Ventrally located sgg clones show a local increase in bristle density due to a failure in lateral inhibition of neuronal fate specification. Bifurcations produced by clones in the tibia or femur are unable to extend beyond the end of the dsegment in which the bifurcation occurred. Dorsally located clones lead to the formation of duplicated legs by respecifying the fates of surrounding cells. Clones in lateral positions cause simple outgrowths of leg that consist entirely of mutant cells. The mutant cells do not affect the fate of the wild type cells around them. Mutant clones in the lateral region of the leg do not respecify cell fate. Pattern respecification occurs only when the clones are located dorsally, near the endogenous anterior-posterior compartment boundary. Duplicated legs always bifurcate from the dorsal side of the endogenous leg.

(Diaz-Benjumea and Cohen, 1994)

Epistatic to N^Ax-1, whereas N^l1N-ts1 is epistatic to sgg³².

(Ruel et al., 1993)

Homozygous clones in the thorax differentiate a cluster of bristles at the site of a single bristle in wild-type flies. In 98% of cases that respond, individual bristles of the same mutant cluster behave similarly to one another and elicit a cleaning response from the same leg. The mutant bristles elicit the same behavioural response as wild-type bristles when stimulated.

(Simpson and Carteret, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sgg³² has visible | recessive | somatic clone phenotype, enhanceable by Su(fu)^LP

(Jia et al., 2002)

NOT Enhancer of

Statement

Reference

sgg[+]/sgg³² is a non-enhancer of abnormal locomotor rhythm phenotype of tim^blind

(Wülbeck et al., 2005)

Suppressor of

Statement

Reference

sgg³² is a suppressor of visible | recessive | somatic clone phenotype of N^Mcd1

(Ramain et al., 2001)

NOT Suppressor of

Statement

Reference

sgg[+]/sgg³² is a non-suppressor of abnormal locomotor rhythm phenotype of tim^blind

(Wülbeck et al., 2005)

Other

Statement

Reference

Pka-C1^E95, sgg³² has visible | recessive phenotype

(Phillips et al., 1999)

cno^mis1, sgg³² has visible phenotype

(Miyamoto et al., 1995)

Phenotype Manifest In

Enhanced by

Statement

Reference

sgg³² has wing | anterior | somatic clone phenotype, enhanceable by Su(fu)^LP

(Jia et al., 2002)

NOT Enhanced by

Statement

Reference

sgg³² has wing phenotype, non-enhanceable by Atg4b^P0997/Atg4b^P0997

(Thumm and Kadowaki, 2001)

Suppressed by

Statement

Reference

sgg³² has macrochaeta & wing | ectopic | somatic clone phenotype, suppressible by pan²

(Schweizer et al., 2003)

NOT suppressed by

Statement

Reference

sgg³² has wing phenotype, non-suppressible by Atg4b^P0997/Atg4b^P0997

(Thumm and Kadowaki, 2001)

sgg³² has phenotype, non-suppressible by Rnor\Gsk3a^hs.PR

(Ruel et al., 1993)

Enhancer of

Statement

Reference

sgg³² is an enhancer of macrochaeta | increased number phenotype of Dronc^C318G.UAS, Scer\GAL4^sca-P309

(Kanuka et al., 2005)

sgg³² is an enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Marygold and Vincent, 2003)

Suppressor of

Statement

Reference

sgg³² is a suppressor of adult thorax & microchaeta phenotype of N^Mcd1

(Ramain et al., 2001)

Other

Statement

Reference

Su(fu)^LP, sgg³² has wing | ectopic | somatic clone phenotype

(Jia et al., 2002)

Pka-C1^E95, sgg³² has prescutum phenotype

(Phillips et al., 1999)

Pka-C1^E95, sgg³² has scutum | anterior phenotype

(Phillips et al., 1999)

cno^mis1, sgg³² has wing vein phenotype

(Miyamoto et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

The sgg³² mutation leads to an enhancement in the number of flies that show extra macrochaetae on the scutellum when Nc^{C318G.Scer\UAS} is expressed under the control of Scer\GAL4^sca-P309.

(Kanuka et al., 2005)

The increase in locomotor rhythm period length seen in tim^blind homozygous flies in free-running conditions is not further increased if the flies also carry sgg³².

(Wülbeck et al., 2005)

Somatic clones homozygous for both sgg³² and pan² show a suppression of the ectopic bristle phenotype seen in sgg³² clones alone.

(Schweizer et al., 2003)

sgg³² Su(fu)^LP double mutant clones in the anterior of the wing organise the formation of a duplicated wing. The double mutant cells form ectopic sensory bristles. Most of the duplicated wing is derived from wild-type cells.

(Jia et al., 2002)

Unlike Pka-C1^E95 clones, sgg³² Pka-C1^E95 double mutant clones in the medial prescutum produce macrochaetae autonomously, and unlike sgg³² clones, the double mutant clones have a symmetrical bristle pattern. Formation of macrochaetae within the double mutant clones is increased relative to sgg³² clones. In the posterior scutum, sgg³² Pka-C1^E95 double mutant clones have a scutellar phenotype very similar to that of Pka-C1^E95 clones. In the anterior scutum, sgg³² Pka-C1^E95 double mutant clones no longer show the non-autonomous induction of macrochaetae or formation of pits characteristic of Pka-C1^E95 clones. Formation of macrochaetae within the double mutant clones is increased relative to sgg³² clones.

(Phillips et al., 1999)

No significant effect on the sca^MSKF mutant phenotype.

(Hu et al., 1995)

cno^mis1 homozygotes that are heterozygous with sgg³² exhibit extra wing vein material.

(Miyamoto et al., 1995)

Xenogenetic Interactions

Statement

Reference

Rnor\Gsk-3α^hs.PR and Rnor\Gsk-3β^hs.PR do not rescue sgg³² at all, even under maximal heat shock. Rnor\Gsk-3β^hs.PR can rescue sgg³² in wing disc clones, therefore can replace sgg during the epidermal-neural cell fate decision, but not at other stages of life cycle that sgg is required.

(Ruel et al., 1993)

Complementation and Rescue Data

Rescued by

sgg³² is rescued by sgg^UASp.cTa

(Takeo et al., 2012)

sgg³² is rescued by sgg^hs.P

(Ruel et al., 1993)

Partially rescued by

sgg³² is partially rescued by sgg^mR39

(Ruel et al., 1993)

sgg³² is partially rescued by sgg^mR10

(Ruel et al., 1993)

Not rescued by

sgg³² is not rescued by sgg^mR46

(Ruel et al., 1993)

Comments

Expression of sgg^{Scer\UAS.P\T.cTa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic defects seen in sgg³² mutant germline clones.

(Takeo et al., 2012)

sgg^mR10 rescues hemizygous males to pharate adults. sgg^mR39 rescues similarly but less efficiently, but sgg^mR46 does not rescue at all.

(Ruel et al., 1993)

Maximal heat shock of mutant alleles bearing sgg^hs.P rescues the sgg³² mutant phenotype. Lower heat shock produces bristle hyperplasia expected of a hypomorphic allele, a phenotype similar to that of N^l1N-ts1 when exposed to the restrictive temperature during early pupal periods.

(Ruel et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Ripoll.

Comments

None of the multiple products of the sgg locus remain in this allele.

(Ruel et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

D127

(Ruel et al., 1993)

l(1)zw3^sgg

sgg-zw3^D127

(Royet and Finkelstein, 1997)

sgg^-

(Lawrence et al., 2002)

sgg³²

sgg^D127

zw3^D127

(Takaesu et al., 2005, McCartney et al., 2001, Thumm and Kadowaki, 2001, Lecuit and Cohen, 1997)

zw3^d127

(McCartney et al., 2001)

zw3<up>sgg D127</up>

(Kalamegham and Siegfried, 2000)

zw3^sggD127

(Wojcik, 2008, Takaesu et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (51)

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