FlyBase Allele Report: Dmel\tim[blind]

General Information

Symbol

Dmel\tim^blind

Species

D. melanogaster

Name

blind

FlyBase ID

FBal0177468

Feature type

allele

Associated gene

Dmel\tim

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wülbeck et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: A1128V. Amino acid replacement: L1131M.

(Wülbeck et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:3,500,027..3,500,027 [-]

Nucleotide change:

C3500027T

Amino acid change:

Reported amino acid change:

A1128V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. One of two mutations in this line. The difference in the reported and annotated positions of the mutations is due to the use of a downstream translation start (23aa) in the current tim gene annotations.

(Wülbeck et al., 2005)

point_mutation

2L:3,500,019..3,500,019 [-]

Nucleotide change:

C3500019A

Amino acid change:

Reported amino acid change:

L1131M

Comment:

(Wülbeck et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm (with Df(2L)tim-02)

(Wülbeck et al., 2005)

abnormal locomotor rhythm (with tim⁰¹)

(Wülbeck et al., 2005)

abnormal locomotor rhythm (with tim⁰³)

(Wülbeck et al., 2005)

abnormal locomotor rhythm (with tim⁰⁴)

(Wülbeck et al., 2005)

abnormal locomotor rhythm | semidominant

(Wülbeck et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Under LD conditions, mutant adults behave similarly to wild-type flies in locomotor rhythm assays. The free-running locomotor activity period of homozygous males is 26 hours, 2 hours longer than that of controls. Heterozygous mutants also show a mild lengthening of the locomotor rhythm period under free-running conditions. The free-running eclosion period of mutant animals is the normal 24 hours. Analysis of the phase response curve (PRC) of tim^blind flies to brief pulses of light indicates that they react with small (1.5 hour) phase delays to light pulses given at ZT15 and ZT17, which normally causes delays of about 3 hours. When the light pulse is given at ZT19, the tim^blind flies react with a 1 hour phase delay, in contrast to wild-type flies, which react with a 3.5 hour phase advance. At ZT21, the mutant flies show robust phase advances, whereas controls already show a reduction in the amount of phase advances at this time. Overall, the tim^blind PRC is blunted in the delay portion, and the transition point (where delay changes to advance) is retarded by about 2 hours.

(Wülbeck et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

tim^blind has abnormal locomotor rhythm phenotype, non-enhanceable by sgg[+]/sgg³²

(Wülbeck et al., 2005)

NOT suppressed by

Statement

Reference

tim^blind has abnormal locomotor rhythm phenotype, non-suppressible by sgg[+]/sgg³²

(Wülbeck et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

More than 80% of adults expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in a tim^blind background rapidly become arrhythmic for locomotor activity after they are placed in constant darkness conditions. The few animals that are rhythmic have a locomotor activity period that is intermediate between that of tim^blind homozygotes and that of adults expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in a wild-type background. The increase in locomotor rhythm period length seen in tim^blind homozygous flies in free-running conditions is not further increased if the flies also carry sgg³².

(Wülbeck et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

tim⁰⁴

(Wülbeck et al., 2005)

tim⁰³

(Wülbeck et al., 2005)

tim⁰¹

(Wülbeck et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tim^blind

(Singh et al., 2019)

Name Synonyms

blind

(Wülbeck et al., 2005)

Secondary FlyBase IDs

References (2)

Report Sections

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