1 day memory retention is normal after spaced training in an olfactory conditioning assay.

The rhythmic change in bouton size that is seen in motor neuron MN5 under LD (12 hour light:12 hour dark) conditions and DD (constant darkness) conditions in wild-type flies is abolished in mutant flies.

Motor neuron MN5 shows increased neuronal branching in mutant flies compared to wild type.

Homozygotes are arrhythmic for locomotor activity under free-running conditions.

The time spent copulating is significantly increased in tim⁰³ mutant couples, compared to wild-type. The time spent copulating by tim⁰¹/tim⁰³ males mated with wild type females is significantly extended compared to wild-type.

Single tim⁰³ mutant females mated once to single tim⁰³ mutant males don't lay significantly less eggs than wild-type flies. However, the percentage of unfertilised eggs from this mating is significantly greater and the number of progeny significantly less than for wild-type flies. Single tim⁰¹/tim⁰³ females mated once to single tim⁰¹/tim⁰³ males produce significantly less progeny than do wild-type flies, even though the number of eggs laid and the percentage of fertilised eggs is not significantly different to wild-type. The numbers of sperm released to the seminal vesicles by tim⁰³ mutant males over the 2 days following eclosion is significantly less than that seen in wild-type males. Single wild-type females mated once to single tim⁰¹/tim⁰³ males lay significantly less eggs and produce significantly less progeny than those mated to wild-type males. tim⁰¹/tim⁰³ males have significantly less sperm in their seminal vesicles 42 hours after eclosion than wild-type males.

Homozygotes and tim⁰¹/tim⁰³ flies show arrhythmic locomotor activity.