FlyBase Allele Report: Dmel\N[gt-10-15]

General Information

Symbol

Dmel\N^gt-10-15

Species

D. melanogaster

Name

FlyBase ID

FBal0269859

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{N.gt-10_15}

PBac{N.gt-10_15}

Also Known As

N^gt-10_15

Key Links

Transgenic product class

missense_variant

(Leonardi et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Leonardi et al., 2011)

Mutagen

in vitro construct

(Leonardi et al., 2011)

Progenitor genotype

N^+t40

(Leonardi et al., 2011)

Carried in construct

P{N.gt-10_15}

PBac{N.gt-10_15}

Cytology

Description

Contains serine-to-alanine mutations in the EGF repeats of region 10_15 to abolish the addition of O-glucose.

(Leonardi et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage | heat sensitive, with N^unspecified

(Leonardi et al., 2011)

some die during pharate adult stage | heat sensitive, with N^unspecified

(Leonardi et al., 2011)

visible | cold sensitive

(Leonardi et al., 2011)

visible | heat sensitive, with N^unspecified

(Leonardi et al., 2011)

Phenotype Manifest In

leg | heat sensitive, with N^unspecified

(Leonardi et al., 2011)

leg joint | heat sensitive, with N^unspecified

(Leonardi et al., 2011)

notal microchaeta | heat sensitive, with N^unspecified

(Leonardi et al., 2011)

wing vein | cold sensitive

(Leonardi et al., 2011)

Detailed Description

Statement

Reference

Animals lacking endogenous N (Df(1)N-54l9/Y) and rescued by one copy of N^gt-10-15 do not exhibit a loss of wing veins.

(Lee et al., 2013)

N^gt-10-15 rescues N^unspecified mutants to pharate adulthood at 30 [o]C, but the rescued animals show a severe loss of bristles on the thorax, loss of leg joints and severe leg shortening. When the rescued animals are raised at 25 [o]C, they readily eclose, the severity of bristle loss is significantly decreased and the legs look normal. At lower temperatures (18-23 [o]C), the rescued animals show a normal bristle pattern and normal legs.

Two copies of N^gt-10-15 significantly rescues the bristle and leg phenotypes of a N^unspecified null allele at 30 [o]C, although not completely, because the rescued animals still display some leg joint defects and die as pharate adults.

Flies with two copies of N^gt-10-15 show extra wing vein tissue and wing vein expansion. However, as the temperature at which the flies are cultured increases, these phenotypes become less severe.

(Leonardi et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

N^gt-10-15 is a suppressor | partially of visible | recessive phenotype of rumi^Δ26

(Leonardi et al., 2011)

N^gt-10-15 is a suppressor of lethal | heat sensitive phenotype of rumi^Δ26

(Leonardi et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

N^gt-10-15 is a suppressor | partially of notal microchaeta phenotype of rumi^Δ26

(Leonardi et al., 2011)

NOT Suppressor of

Statement

Reference

Df(1)N-54l9/N^gt-10-15 is a non-suppressor of wing vein phenotype of Hsap\GXYLT1^UAS.Tag:HA, Scer\GAL4^ap.PU

(Lee et al., 2013)

Df(1)N-54l9/N^gt-10-15 is a non-suppressor of wing margin phenotype of Hsap\XXYLT1^UAS.Tag:HA, Scer\GAL4^nub-AC-62

(Lee et al., 2013)

Df(1)N-54l9/N^gt-10-15 is a non-suppressor of wing vein phenotype of Hsap\XXYLT1^UAS.Tag:HA, Scer\GAL4^nub-AC-62

(Lee et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous rumi^Δ26 mutant animals also carrying N^gt-10-15 fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C.

(Leonardi et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

N^gt-10-15 partially rescues N^unspecified

(Leonardi et al., 2011)

Comments

(Leonardi et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

N^gt-10-15

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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