FlyBase Allele Report: Dmel\ttv[00681b]

In response to nerve stimulation, ttv^00681b mutants exhibit a significantly increased EJP compared with wild-type or heterozygous control larvae (20-25% increase). Spontaneous release events (mEJPs) are substantially reduced compared with heterozygous or wild-type controls, suggesting that loss of Heparan Sulfate biosynthesis affects the spontaneous vesicle release probability in the motoneuron. Post-synaptic mEJP amplitudes are significantly elevated in ttv^00681b mutants relative to heterozygous or wild-type controls.

ttv^00681b mutant larval exhibit locomotion defects, with a significantly reduced number of peristaltic contractions per minute.

ttv^00681b mutant synapses are reduced in size and have significantly fewer boutons per unit muscle area compared with controls. ttv^00681b motoneurons are bigger than normal, with a reduced number of 'buds'. ttv^00681b synapses consist primarily of large boutons without any buds.

ttv^00681b mutants exhibit an increase in activity-dependent endocytosis at the neuromuscular junction.

(Ren et al., 2009)

ttv^00681b/ttv^00681b die at the pupal stage, and animals depleted of maternal product are embryonic lethal.

(Dasgupta et al., 2007)

ttv^00681b mutants display ISNb bypass at a similar rate to wild-type embryos.

(Fox and Zinn, 2005)

ttv^00681b germ-line clone embryos do not display any naked cuticle formation, although the resulting denticle lawn has some diversity.

(Gallet et al., 2003)

Mosaic flies with eyes homozygous for ttv^00681b (clones generated using the "eyFLP" system) have small eyes.

(Amanai and Jiang, 2001)

Homozygous mutant larvae have abnormally large boutons at the terminal arbor synapses of the ISN.

(Kraut et al., 2001)

Homozygous larvae have a marked reduction in heparan sulphate levels, to at least 10-fold less than wild type. Heterozygotes show a slight reduction in heparan sulphate levels. Chondroitin sulphate is unaffected by ttv^00681b.

(Toyoda et al., 2000)

ttv^00681b/Df(2R)trix embryos derived from homozygous female germline clones show absence of naked cuticle.

(The et al., 1999)

Clonal analysis in the wing reveals that ttv is required in the receiving cell for the movement of hh gene product from the sending to the receiving cell.

(Bellaiche et al., 1998)

Germline clones produce eggs with patterning defects: unrescued embryos exhibit mirror image duplication of denticle belts, rescued embryos are wild type. Lethality occurs during the larval and pupal stages.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ttv^00681b has lethal - all die during P-stage phenotype, suppressible by Hsap\EXT1^UASp.GFP/Scer\GAL4^Tub.PU

(Dasgupta et al., 2007)

Enhancer of

Statement

Reference

ttv^00681b is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Sdc²³

(Chanana et al., 2009)

ttv[+]/ttv^00681b is an enhancer of decreased occurrence of cell division | larval stage phenotype of trol¹³

(Park et al., 2003)

ttv[+]/ttv^00681b is an enhancer of decreased occurrence of cell division | larval stage phenotype of trol^p4

(Park et al., 2003)

NOT Enhancer of

Statement

Reference

ttv^00681b, Scer\GAL4^GMR.PF is a non-enhancer of visible | adult stage phenotype of Hsap\INS^C96Y.UAS, Scer\GAL4^GMR.PF

(He et al., 2014)

ttv[+]/ttv^00681b is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Ext2[+], sotv³²⁶, ttv[+], ttv^00681b is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

NOT Suppressor of

Statement

Reference

ttv[+]/ttv^00681b is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Ext2[+], sotv³²⁶, ttv[+], ttv^00681b is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Other

Statement

Reference

sotv^1.8.1, ttv^00681b has abnormal immune response phenotype

(Baron et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

ttv^00681b is an enhancer of fascicle | embryonic stage | ectopic phenotype of Sdc²³

(Chanana et al., 2009)

ttv[+]/ttv^00681b is an enhancer of larval neuroblast | larval stage phenotype of trol^p4

(Park et al., 2003)

NOT Enhancer of

Statement

Reference

ttv^00681b, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\INS^C96Y.UAS, Scer\GAL4^GMR.PF

(He et al., 2014)

ttv[+]/ttv^00681b is a non-enhancer of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Ext2[+], sotv³²⁶, ttv[+], ttv^00681b is a non-enhancer of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Suppressor of

Statement

Reference

ttv^00681b is a suppressor | partially of denticle | germline clone | maternal effect phenotype of Scer\GAL4^en-e16E, hh^UAS.cIa

(Gallet et al., 2003)

NOT Suppressor of

Statement

Reference

ttv[+]/ttv^00681b is a non-suppressor of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Ext2[+], sotv³²⁶, ttv[+], ttv^00681b is a non-suppressor of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Other

Statement

Reference

Scer\GAL4^en-e16E, hh^UAS.cIa, ttv^00681b has denticle | germline clone | maternal effect phenotype

(Gallet et al., 2003)

trol¹³, ttv^00681b has neuroblast | larval stage phenotype

(Park et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The commissural axon phenotype (failure to cross the midline) seen in embryos expressing Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^P52 in a Sema-1a^k13702 null background is not affected if the embryos are also heterozygous for ttv^00681b.

(Cho et al., 2012)

ttv^00681b Ext2^1.8.1 double heterozygotes exhibit increased median survival time compared to controls when infected with group B Streptococcus (both wild-type and GAG-binding impaired mutants) and lower colony counts after infection.

(Wang et al., 2011)

ttv^00681b Ext2^1.8.1 double heterozygotes exhibit reduced susceptibility to infection with the A909 strain of group B streptococcus.

(Baron et al., 2009)

ttv^00681b, Sdc²³ double mutant embryos show a 2-fold increase in ventral midline crossovers of axon fascicles compared to Sdc²³ single mutants.

(Chanana et al., 2009)

Expression of hh^Scer\UAS.cIa with Scer\GAL4^en-e16E in ttv^00681b germ-line clone embryos doesn't rescue naked cuticle formation, but does increase denticle diversity in the ventral cuticle: denticles corresponding to row 2,3,4, and 5 types are all present.

(Gallet et al., 2003)

ttv^00681b/+; trol¹³/trol¹³ larvae have increased numbers of S phase larval neuroblasts per brain lobe than wild-type. Of ttv^00681b/+; trol^p4/trol^p4 larvae, 29% show increased numbers of S phase neuroblasts per brain lobe and while 5% show decreased numbers.

(Park et al., 2003)

Xenogenetic Interactions

Statement

Reference

One copy of ttv^00681b does not enhance the eye phenotype seen when Hsap\INS^{C96Y.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

(He et al., 2014)

Expression of Hsap\EXT1^{Scer\UAS.P\T.T:Avic\GFP} driven by Scer\GAL4^tub.PU partially suppresses pupal lethality in ttv^00681b/ttv^00681b flies, with a small percentage developing and surviving as adult flies.

(Dasgupta et al., 2007)

Complementation and Rescue Data

Complements

ttv^EX3

(Schulze et al., 2005)

ttv^EX187

(Schulze et al., 2005)

ttv^EX16

(Schulze et al., 2005)

ttv^EX364

(Schulze et al., 2005)

ttv^EX296

(Schulze et al., 2005)

ttv^EX265

(Schulze et al., 2005)

ttv^EX5

(Schulze et al., 2005)

ttv^EP2199

(Schulze et al., 2005)

ttv⁰⁵²⁸²

(BDGP Project Members, 1994-1999)

Rescued by

ttv^00681b is rescued by ttv^UAS.Tag:MYC/Scer\GAL4^da.G32

(Ren et al., 2009)

ttv^00681b is rescued by ttv^UAS.Tag:MYC/Scer\GAL4^elav-C155

(Ren et al., 2009)

ttv^00681b is rescued by ttv^UAS.Tag:MYC/Scer\GAL4^G14

(Ren et al., 2009)

ttv^00681b is rescued by ttv^UAS.Tag:MYC/Scer\GAL4^wg.PM

(Gallet et al., 2003)

ttv^00681b is rescued by ttv^hs.PB

(Bellaiche et al., 1998)

Comments

Expression of ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^G14 leads to rescue of ttv^00681b mutant larvae to adulthood or late pupal stages. The number of boutons per unit muscle area and bouton budding are increased to normal levels.

Expression of ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^elav-C155 improves viability of ttv^00681b mutant larvae. The number of boutons per unit muscle area are increased to normal levels while bouton budding is elevated above wild-type levels.

Expression of ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^da.G32 fails to improve viability of ttv^00681b mutant larvae but rescues the abnormally elevated mEJP frequency (but not the EJP amplitude) found in these larvae. The number of boutons relative to muscle area is significantly reduced compared with ttv^00681b mutants.

ttv^00681b mutants exhibit morphological changes in somatic musculature, with mitochondrial distribution affected and altered Golgi organisation.

(Ren et al., 2009)

ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^wg.PM completely rescues the cuticle phenotype of ttv^00681b germ-line clone embryos.

(Gallet et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10949

cn¹ P{PZ}Cyt-b5^00681a P{PZ}ttv^00681b/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Separable from: P{PZ}Cyt-b5^00681a.

(Misra, 2000.8.25)

Comments

Either a null allele or a severe hypomorph.

(Bellaiche et al., 1998)

Complements: L⁰²⁶³⁷. Complements: Tfb1⁰⁶⁹⁴⁹. Complements: Tfb1⁰⁶⁹⁵⁰. Complements: Sec61β⁰⁷²¹⁴. Complements: l(2)k16805^k16805.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

l(2)00681

(Toyoda et al., 2000, Spradling et al., 1999)

l(2)00681⁰⁰⁶⁸¹

tout-velu⁶⁸¹

(Kraut et al., 2001)

tout-velu^l(2)00681

(Toyoda et al., 2000)

ttv00681

(Wang et al., 2011)

ttv⁰⁰⁶⁸¹

(Reynolds-Peterson et al., 2020, Uchino et al., 2013, Gurudatta et al., 2010, Gallet et al., 2006, Schulze et al., 2005, Gallet et al., 2003, Park et al., 2003)

ttv^00681b

(Cho et al., 2012, Chanana et al., 2009, Ren et al., 2009)

ttv⁶⁸¹

(He et al., 2014, Kraut et al., 2001)

ttv^l(2)00681

(Dasgupta et al., 2007, Callejo et al., 2006, Gorfinkiel et al., 2005, Han et al., 2004, Takei et al., 2004, Amanai and Jiang, 2001, The et al., 1999, Bellaiche et al., 1998)

ttv_681

(He et al., 2014)

Name Synonyms

Secondary FlyBase IDs

FBal0122990

References (33)

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