FlyBase Allele Report: Dmel\ttv[UAS.Tag:MYC]

General Information

Symbol

Dmel\ttv^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0102367

Feature type

allele

Associated gene

Dmel\ttv

Associated Insertion(s)

Carried in Construct

P{UAS-ttv.myc}

Also Known As

UAS-ttv-myc, ttv-myc

Key Links

Transgenic product class

wild_type

(The et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(The et al., 1999)

Mutagen

in vitro construct

(The et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-ttv.myc}

Cytology

Description

UASt regulatory sequences drive expression of ttv tagged with six copies of Tag:MYC.

(The et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ttv

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

bouton, with Scer\GAL4^da.G32, ttv^00681b

(Ren et al., 2009)

germline cell, with Scer\GAL4^elav.PLu

(Deshpande et al., 2007)

germline cell, with Scer\GAL4^ptc-559.1

(Deshpande et al., 2007)

germline cell, with Scer\GAL4^wg.PM

(Deshpande et al., 2007)

Detailed Description

Statement

Reference

Expression of ttv^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hh-Gal4 has little effect on germ cell migration in embryos.

Expression of ttv^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1 results in germ cell migration defects in embryos; most of the germ cells are scattered in the posterior of the embryo and fail to coalesce with the somatic gonadal precursor cells. In a subset of embryos germ cells are clustered in the middle of the embryo, resulting in three germ cell clusters; two in the appropriate location on either side of the embryo in parasegment 10 and an additional cluster more anteriorly.

Expression of ttv^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^wg.PM results in germ cell migration defects in embryos.

Expression of ttv^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PLu results in germ cell migration defects in embryos. A significant number of germ cells that do not coalesce with the somatic gonadal precursor cells are found proximal to either the central nervous system or the ventral nerve cord.

Expression of ttv^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^h-1J3 results in a variety of germ cell migration defects in embryos. In approximately half the embryos, germ cells are seen in the vicinity of parasegments 10-12 but fail to coalesce with the somatic gonadal precursor cells. In approximately one quarter of the embryos germ cells are scattered widely throughout the embryos and in the remaining embryos there is a "pseudogonad"-like assembly in the middle of the embryo.

(Deshpande et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

ttv^UAS.Tag:MYC/Scer\GAL4^h-1J3 is a non-suppressor of ventral denticle belt | germline clone | maternal effect phenotype of botv¹⁰³

(Han et al., 2004)

ttv^UAS.Tag:MYC/Scer\GAL4^h-1J3 is a non-suppressor of embryonic/first instar larval cuticle | germline clone | maternal effect phenotype of botv¹⁰³

(Han et al., 2004)

ttv^UAS.Tag:MYC/Scer\GAL4^h-1J3 is a non-suppressor of ventral denticle belt | germline clone | maternal effect phenotype of sotv⁴⁴

(Han et al., 2004)

ttv^UAS.Tag:MYC/Scer\GAL4^h-1J3 is a non-suppressor of embryonic/first instar larval cuticle | germline clone | maternal effect phenotype of sotv⁴⁴

(Han et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ttv^UAS.Tag:MYC/Scer\GAL4^da.G32 rescues ttv^00681b

(Ren et al., 2009)

ttv^UAS.Tag:MYC/Scer\GAL4^elav-C155 rescues ttv^00681b

(Ren et al., 2009)

ttv^UAS.Tag:MYC/Scer\GAL4^G14 rescues ttv^00681b

(Ren et al., 2009)

ttv^UAS.Tag:MYC/Scer\GAL4^wg.PM rescues ttv^00681b

(Gallet et al., 2003)

ttv^UAS.Tag:MYC/Scer\GAL4^unspecified rescues ttv^unspecified

(The et al., 1999)

Partially rescues

ttv^UAS.Tag:MYC/Scer\GAL4^h-1J3 partially rescues ttv⁶³

(Han et al., 2004)

Comments

Expression of ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^G14 leads to rescue of ttv^00681b mutant larvae to adulthood or late pupal stages. The number of boutons per unit muscle area and bouton budding are increased to normal levels.

Expression of ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^elav-C155 improves viability of ttv^00681b mutant larvae. The number of boutons per unit muscle area are increased to normal levels while bouton budding is elevated above wild-type levels.

Expression of ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^da.G32 fails to improve viability of ttv^00681b mutant larvae but rescues the abnormally elevated mEJP frequency (but not the EJP amplitude) found in these larvae. The number of boutons relative to muscle area is significantly reduced compared with ttv^00681b mutants.

(Ren et al., 2009)

ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^wg.PM completely rescues the cuticle phenotype of ttv^00681b germ-line clone embryos.

(Gallet et al., 2003)

Rescues the segment polarity phenotype of ttv^unspecified embryos.

(The et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ttv^{Scer\UAS.T:Hsap\MYC}

ttv^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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