FlyBase Allele Report: Dmel\Itpr[wc361]

General Information

Symbol

Dmel\Itpr^wc361

Species

D. melanogaster

Name

FlyBase ID

FBal0156339

Feature type

allele

Associated gene

Dmel\Itpr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Joshi et al., 2004, Deshpande et al., 2000)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Joshi et al., 2004)

Amino acid replacement: R2814term.

(Joshi et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:5,538,601..5,538,601 [+]

Nucleotide change:

C5538601T

Reported nucleotide change:

C?T

Amino acid change:

R2813term | Itp-r83A-PA; R2822term | Itp-r83A-PB; R2813term | Itp-r83A-PC

Reported amino acid change:

R2814term

(Joshi et al., 2004, Deshpande et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autosomal dominant cerebellar ataxia

CEC with Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Psn^B3

(McBride et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight (with Itpr^ka1091)

(Richhariya et al., 2017, Banerjee et al., 2004)

abnormal flight (with Itpr^sa54)

(Banerjee et al., 2004)

abnormal flight (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020)

abnormal flight (with Itpr^wc703)

(Banerjee et al., 2004)

abnormal learning | adult stage | male

(McBride et al., 2010)

flightless (with Itpr^ug3)

(Banerjee et al., 2004)

flightless | partially (with Itpr^wc703)

(Banerjee et al., 2006)

lethal | cold sensitive (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020)

lethal | recessive

(Joshi et al., 2004)

partially lethal (with Itpr^sa54)

(Joshi et al., 2004)

partially lethal (with Itpr^ug3)

(Joshi et al., 2004)

partially lethal | cold sensitive (with Itpr^ka901)

(Joshi et al., 2004)

partially lethal | cold sensitive (with Itpr^wc703)

(Joshi et al., 2004)

partially lethal - majority live (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020)

viable (with Itpr^ug3)

(Deshpande et al., 2000)

viable (with Itpr^wc703)

(Banerjee et al., 2006)

visible | adult stage (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020)

Phenotype Manifest In

wing (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020)

Detailed Description

Statement

Reference

Itpr^ug3/Itpr^wc361 adults show a "spread out" wing posture.

(Balasubramanian and Srinivasan, 2020)

Itp-r83A^wc361/Itp-r83A^ka1091 heterozygous adults display significant decrease in flight ability (measured as flight time in a single flight assay on tethered flies).

(Richhariya et al., 2017)

Itp-r83A^wc361/+ 30-day-old adult males display learning defects as they fail to demonstrate significant decrease in courtship activity when paired with an mated unreceptive female but their short-term (60 min) memory is not impaired.

(McBride et al., 2010)

The spontaneous firing rate seen in dorsal-longitudinal muscles of Itp-r83A^wc361/Itp-r83A^wc703 adults is normal. These adults can be divided into fliers and non-fliers (assayed using a cylinder drop test). About 30% are non-fliers - exhibiting only brief rhythmic flight patterns that are usually not sustained beyond 5 seconds in response to stimulation with an air puff. In contrast, the non-fliers exhibit sustained flight patterns (>30 seconds) in response to the same stimulus.

(Banerjee et al., 2006)

Itp-r83A^wc703/Itp-r83A^wc361, Itp-r83A^ka1091/Itp-r83A^wc361 and Itp-r83A^sa54/Itp-r83A^wc361 flies all show normal wing posture but do show a significant degree of flightlessness.

(Banerjee et al., 2004)

Itp-r83A^wc361/Itp-r83A^ug3 transheterozygotes are adaptation response defective.

(Deshpande et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, enhanceable by Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, enhanceable by Gα49B[+]/Gαq^221c

(Banerjee et al., 2006)

Suppressed by

Statement

Reference

Itpr^wc361/Itpr^ug3, NUCB1^c01508, Orai^k11505 has abnormal flight phenotype, suppressible | partially by SERCA[+]/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has visible | adult stage phenotype, suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has visible | adult stage phenotype, suppressible by NUCB1^NIG.32190R/Scer\GAL4^Ddc.PL

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has increased mortality during development phenotype, suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by SERCA[+]/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by SERCA[+]/SERCA^Kum170, NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by SERCA[+]/NUCB1^c01508/SERCA^Kum170/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, suppressible by Orai[+]/Orai^k11505, NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, suppressible by SERCA[+]/Orai[+]/Orai^k11505/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, suppressible by Orai[+]/NUCB1^NIG.32190R/Orai^k11505/Scer\GAL4^Ddc.PL

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, suppressible by NUCB1^c01508/Orai[+]/Orai^k11505/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ka1091 has abnormal flight phenotype, suppressible | partially by Scer\GAL4^elav-C155/Rala^UAS.cRa

(Richhariya et al., 2017)

Itpr^wc361 has abnormal learning | male | adult stage phenotype, suppressible by Psn^B3/Psn[+]

(McBride et al., 2010)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has flightless | partially phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has abnormal neurophysiology phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

NOT suppressed by

Statement

Reference

Itpr^wc361/Itpr^ug3, Orai[+]/Orai^k11505, SERCA[+]/SERCA^Kum170 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, non-suppressible by SERCA[+]/NUCB1^c01508/SERCA^Kum170/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, non-suppressible by SERCA[+]/SERCA^Kum170, NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, non-suppressible by NUCB1^NIG.32190R/Scer\GAL4^αTub84B.PL

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, non-suppressible by SERCA[+]/NUCB1^NIG.32190R/Scer\GAL4^Ddc.PL/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, non-suppressible by SERCA[+]/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has abnormal flight phenotype, non-suppressible by Orai[+]/Orai^k11505

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361 has abnormal learning | male | adult stage phenotype, non-suppressible by Psn^I2/Psn[+]

(McBride et al., 2010)

Suppressor of

Statement

Reference

Itpr^wc361/Itp-r83A[+] is a suppressor of abnormal learning | male | adult stage | progressive phenotype of Psn^B3

(McBride et al., 2010)

Itpr^wc361/Itp-r83A[+] is a suppressor of abnormal memory | male | adult stage | progressive phenotype of Psn^B3

(McBride et al., 2010)

Itpr^wc361/Itp-r83A[+] is a suppressor of abnormal memory | male | adult stage | progressive phenotype of Psn^I2

(McBride et al., 2010)

NOT Suppressor of

Statement

Reference

Itpr^wc361/Itp-r83A[+] is a non-suppressor of abnormal learning | male | adult stage | progressive phenotype of Psn^I2

(McBride et al., 2010)

Other

Statement

Reference

Itpr^wc361/Itpr^ug3, NUCB1^c01508, Orai^k11505 has abnormal flight phenotype

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3, SERCA[+]/SERCA^Kum170 has viable phenotype

(Balasubramanian and Srinivasan, 2020)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has partially lethal - majority die phenotype

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype

(Banerjee et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, enhanceable by Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Suppressed by

Statement

Reference

Itpr^wc361/Itpr^ug3 has wing phenotype, suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3 has wing phenotype, suppressible by NUCB1^NIG.32190R/Scer\GAL4^Ddc.PL

(Balasubramanian and Srinivasan, 2020)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has wing phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Other

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype

(Banerjee et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The severely reduced flight ability (measured as flight time in a single flight assay on tethered flies) characteristic for Itp-r83A^wc361/Itp-r83A^ka1091 heterozygous flies is significantly ameliorated by Scer\GAL4^elav-C155-driven expression of Rala^Scer\UAS.cRa in the mutant background.

(Richhariya et al., 2017)

The learning defects (measured in a courtship behavior assay) observed in the Psn^B3, Psn^I2 or the Itp-r83A^wc361 single heterozygote 30 day-old males are suppressed in the Psn^B3/+;Itp-r83A^wc361/+ double heterozygotes but not in the Psn^I2/+;Itp-r83A^wc361/+ males. Combination with a single copy of Itp-r83A^wc361 can however restore the short-term memory defects characteristic for both Psn^B3 and Psn^I2 aged heterozygote males.

(McBride et al., 2010)

The viability of Itp-r83A^wc703/Itp-r83A^wc361 animals is unaffected by sl¹/sl¹ or sl²/sl². It is also unaffected by Gα49B^221c/+, but the resulting adults have abnormal wing posture, an enhanced incidence of flight defects and mild endogenous hyperactivity of spontaneous firing recorded from the dorsal longitudinal muscles. Wing posture and flight defect phenotypes are enhanced and viability is reduced if Plc21C^k31911/+ is also present, but partially suppressed by Gα49B^Scer\UAS.cRa; Scer\GAL4^Ddc.PL and completely or largely suppressed by Ca-P60A^Kum170/+, even when Plc21C^k31911/+ is also present.