FlyBase Allele Report: Dmel\Psn[B3]

General Information

Symbol

Dmel\Psn^B3

Species

D. melanogaster

Name

FlyBase ID

FBal0097197

Feature type

allele

Associated gene

Dmel\Psn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Ye et al., 1999)

Mutagen

ethyl methanesulfonate

(Lukinova et al., 1999, Ye et al., 1999)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G to A. Amino acid replacement: G516E. Mutation is in the C-terminal tail.

(Lukinova et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:20,434,970..20,434,970 [+]

Nucleotide change:

G20434970A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

G516E

(Lukinova et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(McBride et al., 2010)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^695.UAS

(Greeve et al., 2004)

model of Alzheimer's disease

is ameliorated by mGluR^112b

(McBride et al., 2010)

is ameliorated by Itpr^wc361

(McBride et al., 2010)

is ameliorated by Ipp¹

(McBride et al., 2010)

is ameliorated by Itpr^wc703

(McBride et al., 2010)

is ameliorated by Ipp³

(McBride et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

There is no significant difference in the normalised number of synaptic boutons or the lengths of synaptic branches between mutant and wild-type type 1 glutamatergic larval neuromuscular junctions. The size of type 1b synaptic boutons is significantly reduced at the mutant larval neuromuscular junction compared to controls.

(Kim et al., 2010)

Young (less than 10 days old) males heterozygous for Psn^I2 do not show any defects in their courtship behavior or locomotor activity compared to wild-type. Their learning index during training with mated unreceptive female is also normal as is their immediate recall memory (assayed by measuring courtship behavior of a male which has just completed a training with a mated unreceptive female toward a receptive virgin female) and 60 min short-term memory (courtship of the trained male is assayed after a 60 min break).

30-day old males heterozygous for Psn^B3 also do not show any defects in the level or quality of naive courtship, locomotor activity, phototaxis or chemotaxis, however they display learning defects (fail to demonstrate the typical decrease in courtship activity when paired with an mated unreceptive female) as well as loss of short-term (60 min) memory is impaired (immediate recall memory is normal though).

(McBride et al., 2010)

Psn^B3/Psn^I2 wing discs lack all cell fates distal to the wg expression domain, i.e. the wing pouch and margin.

(Klein et al., 2000)

Hemizygotes show late prepupal lethality, at the P4(i) prepupa stage.

(Lukinova et al., 1999)

A dramatic increase in the number of cells undergoing apoptosis is seen in certain regions of homozygous third larval instar eye discs. High levels of apoptosis are also seen in the wing and leg discs.

(Ye and Fortini, 1999)

Mutant larvae secrete a pupal case and complete the last stages of larval development but do not form any adult structures. They collapse into a homogeneous oily mass within the pupal case. The phenotype closely resembles that of Su(H) mutants. Wing margin structures are missing from mutant wing discs. Size of wing pouch is reduced. Clusters of supernumerary SOP cells arise at the position of normal SOP cell emergence. Lateral inhibition in the clusters is impaired. Embryos deficient for maternal and zygotic Psn, derived by fertilization of Psn^S3 germline clone-derived eggs by Psn^B3 sperm, show neural hyperplasia of the embryonic nervous system, and the maternal phenotype is only weakly rescued by wild-type paternal zygotic Psn function. Third instar larval eye discs show a severe mutant phenotype. Remnants of morphogenetic furrow are evident in only some eye discs.

(Ye et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Psn^B3 has abnormal learning | male | adult stage | progressive phenotype, suppressible by mGluR^112b/mGluR[+]

(McBride et al., 2010)

Psn^B3 has abnormal memory | male | adult stage | progressive phenotype, suppressible by mGluR^112b/mGluR[+]

(McBride et al., 2010)

Psn^B3 has abnormal learning | male | adult stage | progressive phenotype, suppressible by Ipp³/Ipp[+]

(McBride et al., 2010)

Psn^B3 has abnormal memory | male | adult stage | progressive phenotype, suppressible by Ipp³/Ipp[+]

(McBride et al., 2010)

Psn^B3 has abnormal learning | male | adult stage | progressive phenotype, suppressible by Ipp¹/Ipp[+]

(McBride et al., 2010)

Psn^B3 has abnormal memory | male | adult stage | progressive phenotype, suppressible by Ipp¹/Ipp[+]

(McBride et al., 2010)

Psn^B3 has abnormal learning | male | adult stage | progressive phenotype, suppressible by Itpr^wc361/Itp-r83A[+]

(McBride et al., 2010)

Psn^B3 has abnormal memory | male | adult stage | progressive phenotype, suppressible by Itpr^wc361/Itp-r83A[+]

(McBride et al., 2010)

Psn^B3 has abnormal learning | male | adult stage | progressive phenotype, suppressible by Itpr^wc703/Itp-r83A[+]

(McBride et al., 2010)

Psn^B3 has abnormal memory | male | adult stage | progressive phenotype, suppressible by Itpr^wc703/Itp-r83A[+]

(McBride et al., 2010)

Psn^B3 has abnormal memory | male | adult stage | progressive phenotype, suppressible by Itpr^90B.0/Itp-r83A[+]

(McBride et al., 2010)

Fmr1³, Psn^B3/Psn[+] has abnormal courtship behavior phenotype, suppressible by Fmr1^+t14

(McBride et al., 2010)

NOT suppressed by

Statement

Reference

Psn^B3 has abnormal learning | male | adult stage | progressive phenotype, non-suppressible by mGluR^2b/mGluR[+]

(McBride et al., 2010)

Psn^B3 has abnormal memory | male | adult stage | progressive phenotype, non-suppressible by mGluR^2b/mGluR[+]

(McBride et al., 2010)

Psn^B3 has abnormal learning | male | adult stage | progressive phenotype, non-suppressible by Itpr^90B.0/Itp-r83A[+]

(McBride et al., 2010)

Suppressor of

Statement

Reference

Psn^B3/Psn[+] is a suppressor of abnormal learning | male | adult stage phenotype of Ipp¹

(McBride et al., 2010)

Psn^B3/Psn[+] is a suppressor of abnormal learning | male | adult stage phenotype of Ipp³

(McBride et al., 2010)

Psn^B3/Psn[+] is a suppressor of abnormal learning | male | adult stage phenotype of Itpr^wc361

(McBride et al., 2010)

Psn^B3/Psn[+] is a suppressor of abnormal learning | male | adult stage phenotype of Itpr^wc703

(McBride et al., 2010)

Psn^B3/Psn[+] is a suppressor of increased cell death phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PF

(Greeve et al., 2004)

NOT Suppressor of

Statement

Reference

Psn^B3/Psn[+] is a non-suppressor of abnormal learning | male | adult stage phenotype of Itpr^90B.0

(McBride et al., 2010)

Other

Statement

Reference

Fmr1³, Psn^B3/Psn[+] has abnormal courtship behavior phenotype

(McBride et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Psn^I2/Psn^B3 has wing disc phenotype, suppressible by H^E31

(Klein et al., 2000)

Psn^I2/Psn^B3 has wing margin phenotype, suppressible by H^E31

(Klein et al., 2000)

Psn^I2/Psn^B3 has wing margin phenotype, suppressible by N^int.SH.UAS/Scer\GAL4^dpp.blk1

(Klein et al., 2000)

Psn^I2/Psn^B3 has wing disc phenotype, suppressible by N^int.SH.UAS/Scer\GAL4^dpp.blk1

(Klein et al., 2000)

Psn^B3 has phenotype, suppressible by N^int.hs

(Ye and Fortini, 1999)

NOT suppressed by

Statement

Reference

Psn^I2/Psn^B3 has wing disc phenotype, non-suppressible by Delta^UAS.cHa/Scer\GAL4^dpp.blk1

(Klein et al., 2000)

Psn^I2/Psn^B3 has wing margin phenotype, non-suppressible by Delta^UAS.cHa/Scer\GAL4^dpp.blk1

(Klein et al., 2000)

Suppressor of

Statement

Reference

Psn^B3/Psn[+] is a suppressor of eye photoreceptor cell phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PF

(Greeve et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The age-onset learning and memory defects characteristic for Psn^B3 heterozygote males are suppressed by combination with a single copy of the mGluR^112b allele but not with the mGluR^2b allele.

The learning defects (measured by assessing courtship behavior) observed in Psn^B3, Ipp[3] or Ipp¹ single heterozygote 30 day-old males are suppressed in both Psn^B3/+;Ipp³/+ and Psn^B3/+;Ipp¹/+ double heterozygotes. Combination with a single copy of either Ipp³ or Ipp¹ can also restore the short-term memory defects characteristic for aged Psn^B3 heterozygote males.

The learning defects (measured by assessing courtship behavior) observed in Psn^B3, Itp-r83A^wc361, Itp-r83A^wc703 and Itp-r83A^90B.0 single heterozygote 30 day-old males are suppressed in the Psn^B3/+;Itp-r83A^wc361/+, Psn^B3/+;Itp-r83A^wc703/+ but not in the Psn^B3/+;Itp-r83A^90B.0/+ double heterozygotes. Combination with a single copy of Itp-r83A^wc361 or Itp-r83A^wc703 or Itp-r83A^90B.0 can also restore the short-term memory defects characteristic for aged Psn^B3 heterozygote males.

The courtship behavior of 5-day-old Fmr1³/+;Psn^B3/+ double heterozygous males is severely impaired compared to either of the single heterozygotes that display normal courtship. This defect in naive courtship behavior can be rescued by combination with Fmr1^+t14 allele.

(McBride et al., 2010)

Xenogenetic Interactions

Statement

Reference

A Psn^B3/+ background rescues the age-dependent retinal neurodegeneration phenotype seen when Hsap\APP^695.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

(Greeve et al., 2004)

Complementation and Rescue Data

Rescued by

Psn^B3 is rescued by Psn^+14.PEPC

(Kim et al., 2010)

Psn^B3 is rescued by Psn^loop.Tag:MYC

(Stempfle et al., 2010)

Psn^B3 is rescued by Psn^N.Tag:MYC

(Stempfle et al., 2010)

Psn^B3 is rescued by Psn^PEPC.S

(Seidner et al., 2006)

Comments