FlyBase Allele Report: Dmel\Gαq[221c]

General Information

Symbol

Dmel\Gαq^221c

Species

D. melanogaster

Name

FlyBase ID

FBal0219375

Feature type

allele

Associated gene

Dmel\Gαq

Associated Insertion(s)

Carried in Construct

Also Known As

dgq^221c

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Banerjee et al., 2006)

Progenitor genotype

Gαq^KG07290

(Banerjee et al., 2006)

Cytology

Description

Imprecise excision of P{SUPor-P}Gα49B^KG07290 has produced a 359bp deletion in the region of the Gα49B translation start site.

(Banerjee et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Gαq^221c/+ mutant larvae show a small decrease in spontaneous miniature neurotransmission. A compensatory increase in quantal content means that evoked neurotransmission is unaffected.

(Brusich et al., 2015)

1 day old flies in which the whole eye is mutant for Gα49B^221c do not show any detectable rhabdomere defects.

(Pinal and Pichaud, 2011)

Homozygous ab3A ORNs generated using MARCM show a normal odour response to methyl butyrate and to butyric acid.

(Yao and Carlson, 2010)

Homozygotes die as either first or early second instar larvae.

Animals containing large homozygous clones in the antenna have normal numbers of trichoidea, coeloconica, and basiconica sensilla.

The electroantennograms of animals containing large homozygous clones in the antenna have a reduced amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate).

The electroantennograms of Gα49B^221c/+ animals show a small but significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate).

Gα49B¹/Gα49B^221c flies do not show any defects in electroantennogram recordings.

(Kain et al., 2008)

Gα49B^f04219 homozygotes die at around 10-12 hours post-hatching.

(Banerjee et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gαq^221c has abnormal neurophysiology | dominant phenotype, enhanceable by Plc21C[+]/Plc21C^A

(Kain et al., 2008)

Gαq^221c has abnormal neurophysiology | dominant phenotype, enhanceable by Plc21C^k31911/Plc21C[+]

(Kain et al., 2008)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, enhanceable by Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Suppressed by

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has flightless | partially phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has abnormal neurophysiology phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

Enhancer of

Statement

Reference

Gαq^221c/Galphaq[+] is an enhancer | female limited of partially lethal - majority die phenotype of Scer\GAL4^elav-C155, cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

Galpha49B[+]/Gαq^221c is an enhancer of abnormal neurophysiology | dominant phenotype of Plc21C^k31911

(Kain et al., 2008)

Galpha49B[+]/Gαq^221c is an enhancer of abnormal neurophysiology | dominant phenotype of Plc21C^A

(Kain et al., 2008)

Gα49B[+]/Gαq^221c is an enhancer of flightless | partially phenotype of Itpr^wc703/Itpr^wc361

(Banerjee et al., 2006)

Suppressor of

Statement

Reference

Galpha49B[+]/Gαq^221c is a suppressor of abnormal neurophysiology phenotype of rdgA³

(Kain et al., 2008)

Galpha49B[+]/Gαq^221c is a suppressor of abnormal neurophysiology phenotype of rdgA¹

(Kain et al., 2008)

Other

Statement

Reference

Gαq^221c/Galphaq[+], GluRIIA^SP16 has abnormal neurophysiology phenotype

(Brusich et al., 2015)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has partially lethal - majority die phenotype

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype

(Banerjee et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, enhanceable by Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Suppressed by

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has wing phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Other

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype

(Banerjee et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Gαq^221c prevents the compensatory increase in quantal content seen in the NMJs of GluRIIA^SP16 mutant larvae with reduced quantal size, resulting in impaired evoked neurotransmission. There is no increase in quantal content compared with Gαq^221c/+ controls.

(Brusich et al., 2015)

The electroantennograms of Gα49B^221c/Plc21C^A and Gα49B^221c/Plc21C^k31911 double heterozygous animals show a significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate) and the reduction in the double heterozygotes is significantly greater than that expected to arise from a mere additive effect of the single heterozygous phenotypes.

Introduction of a single copy of Gα49B^221c to rdgA¹ or rdgA³ homozygotes significantly rescues the electroantennogram responses of the homozygotes.

(Kain et al., 2008)

The viability of Itp-r83A^wc703/Itp-r83A^wc361 animals is unaffected by Gα49B^221c/+, but the resulting adults have abnormal wing posture, an enhanced incidence of flight defects and mild endogenous hyperactivity of spontaneous firing recorded from the dorsal longitudinal muscles. Wing posture and flight defect phenotypes are enhanced and viability is reduced if Plc21C^k31911/+ is also present but partially suppressed by Gα49B^Scer\UAS.cRa; Scer\GAL4^Ddc.PL and completely or largely suppressed by Ca-P60A^Kum170/+, even when Plc21C^k31911/+ is also present.

(Banerjee et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Gαq^221c is rescued by Scer\GAL4^{Orco.2.642.Hsim\VP22}/Gαq^UAS.cRa

(Kain et al., 2008)

Comments

Expression of Gα49B^Scer\UAS.cRa under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} rescues the reduced electroantennogram amplitude in response to odours of animals containing large Gα49B^221c homozygous clones in the antenna.

Expression of Gα49B^Scer\UAS.cRa under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} only during adulthood (expression is inhibited before this stage by expression of Scer\GAL80^ts.αTub84B at 18[o]C, and the animals are shifted to 29[o]C to inactivate Scer\GAL80^ts.αTub84B a few hours before eclosion) also rescues the reduced electroantennogram amplitude in response to odours of animals containing large Gα49B^221c homozygous clones in the antenna.

(Kain et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30744

w^*; Gαq^221c/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Gq^221c

(Brusich et al., 2018)

Gα49B^221c

Gα_q^221C

(Yao and Carlson, 2010)

Gαq^221c

(Gu et al., 2020, Brusich et al., 2015)

dgq^221c

(Pinal and Pichaud, 2011, Kain et al., 2009, Kain et al., 2008, Banerjee et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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