FlyBase Allele Report: Dmel\Mmp2[W307stop]

General Information

Symbol

Dmel\Mmp2^W307stop

Species

D. melanogaster

Name

FlyBase ID

FBal0150747

Feature type

allele

Associated gene

Dmel\Mmp2

Associated Insertion(s)

Carried in Construct

Also Known As

mmp2^W307*, Mmp2^W307

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Page-McCaw et al., 2003)

Mutagen

ethyl methanesulfonate

(Page-McCaw et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: W307term.

(Page-McCaw et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,613,582..9,613,582 [-]

Nucleotide change:

G9613582A

Amino acid change:

W307term | Mmp2-PB; W155term | Mmp2-PC; W199term | Mmp2-PD

Reported amino acid change:

W307term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.

(Page-McCaw et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage

(Raza et al., 2017)

abnormal cell shape | embryonic stage

(Raza et al., 2017)

abnormal neuroanatomy | embryonic stage

(Miller et al., 2011, Miller et al., 2008)

abnormal neuroanatomy | embryonic stage (with Df(2R)Uba1-Mmp2)

(Miller et al., 2008)

abnormal neuroanatomy | pupal stage

(Kuo et al., 2005)

increased cell number | adult stage | female | heat sensitive (with Mmp2^Y53N)

(Wang and Page-McCaw, 2014)

increased cell number | oogenesis | heat sensitive (with Mmp2^Y53N)

(Wang and Page-McCaw, 2014)

increased occurrence of cell division | adult stage | female | heat sensitive (with Mmp2^Y53N)

(Wang and Page-McCaw, 2014)

lethal | pupal stage

(Page-McCaw et al., 2003)

lethal - all die before end of pupal stage

(Kuo et al., 2005)

lethal - all die before end of pupal stage (with Df(2R)Uba1-Mmp2)

(Glasheen et al., 2009)

lethal - all die before end of pupal stage (with Mmp2^Df)

(Page-McCaw et al., 2003)

partially lethal - majority die | larval stage

(Page-McCaw et al., 2003)

some die during pupal stage

(Kuo et al., 2005)

some die during pupal stage (with Df(2R)Uba1-Mmp2)

(Glasheen et al., 2009)

some die during pupal stage (with Mmp2^Df)

(Page-McCaw et al., 2003)

Phenotype Manifest In

dendritic arborizing neuron | P-stage

(Kuo et al., 2005)

dorsal air sac primordium | third instar larval stage

(Guha et al., 2009)

embryonic/larval proventriculus | P-stage

(Page-McCaw et al., 2003)

embryonic/larval tracheal transverse connective | third instar larval stage

(Guha et al., 2009)

embryonic heart cardioblast

(Raza et al., 2017)

filopodium | embryonic stage

(Raza et al., 2017)

follicle stem cell | heat sensitive (with Mmp2^Y53N)

(Wang and Page-McCaw, 2014)

gastric caecum | P-stage

(Page-McCaw et al., 2003)

germarium region 2b | heat sensitive (with Mmp2^Y53N)

(Wang and Page-McCaw, 2014)

head | pupal stage (with Df(2R)Uba1-Mmp2)

(Glasheen et al., 2009)

heart primordium

(Raza et al., 2017)

lamellipodium | embryonic stage

(Raza et al., 2017)

larval intersegmental nerve branch ISNb of A1-7

(Miller et al., 2008)

larval intersegmental nerve branch ISNb of A1-7 (with Df(2R)Uba1-Mmp2)

(Miller et al., 2008)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Miller et al., 2011)

larval segmental nerve branch SNa of A1-7

(Miller et al., 2008)

larval segmental nerve branch SNa of A1-7 (with Df(2R)Uba1-Mmp2)

(Miller et al., 2008)

stalk follicle cell | increased number | heat sensitive (with Mmp2^Y53N)

(Wang and Page-McCaw, 2014)

wing disc | third instar larval stage

(Guha et al., 2009)

Detailed Description

Statement

Reference

Mmp2^W307stop/Mmp2^W307stop mutant embryos exhibit defects in heart formation, with disorganized arrangement of cardioblasts, and a decrease in migration velocity of cardioblasts to the midline, with a significant decrease in the number of filopodia and lamellipodia at the leading edge; cardioblasts fail to change shape to form a medial pocket, fail to contact and adhere to their contralateral partners, and a lumen fails to form, in contrast to wild type.

(Raza et al., 2017)

Mmp2^W307stop/Mmp2²¹⁸ transheterozygotes exhibit a decrease in lifespan compared to controls.

(Wang and Page-McCaw, 2014)

Homozygous mutant Mmp2^W307stop embryos exhibit defasciculation defects in 81% of hemisegments.

(Miller et al., 2011)

No Mmp1^W439stop/Df(2R)Uba1-Mmp2 mutant third instar larvae display broken tracheal dorsal trunks. 94% survive to pupariation. Over 50% of dissected pupae show defects in head eversion.

(Glasheen et al., 2009)

Mmp2^W307stop mutants show ectopic basal lamina formation around tunneled tracheae and dorsal air sac primordia of the wing disc. The depth at which the disc-associated transverse connective and dorsal air sac position within the wing disc is altered in these animals. Tunneled trachea and dorsal air sac primordium also show ectopic extrusions from the plane of the imaginal disc. Extension and growth of the dorsal air sac primordia is stunted in these mutants, and the developing air sac tubes are filled with polarised epithelial cells.

(Guha et al., 2009)

Mmp2^W307stop homozygous embryos display marked defects in ISNb fasciculation - 85% of hemisegments display either loosely fasciculated or excessive branching of ISNb. SNa fasciculation is also decreased.

Mmp2^W307stop/Df(2R)Uba1-Mmp2 embryos display marked defects in ISNb fasciculation - 74% of hemisegments display either loosely fasciculated or excessive branching of ISNb. SNa fasciculation is also decreased.

(Miller et al., 2008)

Mosaic animals in which the eyes are homozygous for Mmp2^W307stop show normal location of photoreceptor nuclei in the retina.

(Curtin et al., 2005)

Mmp2^W307stop mutants exhibit abundant C4da neuron larval dendrites after head eversion, with approximately 8% retained after head eversion. Whereas in wild-type pupae at 20hrs after pupal formation all larval dendrites from C4da neurons are cleared from the extracellular space, in Mmp2^W307stop mutants, larval dendrites that are severed from the soma remain. These larval dendrites persist to much later stages at 35 hours after pupal formation, just before the lethal phase of Mmp2^W307stop at midpupariation.

(Kuo et al., 2005)

83% of Mmp2^W307stop mutant animals survive to 3rd instar, and 72% pupariate, but only 15% undergo head eversion, 2% develop head bristles and none eclose. 96% of Mmp2^W307stop/Mmp2^Df animals survive to third instar, and 89% pupariate, but only 18% undergo head eversion, 7% develop head bristles and none eclose. Mmp2^W307stop mutant pupae retain their gastric caeca and proventriculus, even 22 hr after puparium formation.

(Page-McCaw et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mmp2^Y53N/Mmp2^W307stop has increased cell number | adult stage | female | heat sensitive phenotype, enhanceable by dlp^UAS.cBa/Scer\GAL4^C587

(Wang and Page-McCaw, 2014)

Mmp2^W307stop has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by frac^Δ1/frac[+]

(Miller et al., 2011)

Mmp2^W307stop has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by Mmp1^Q112stop

(Miller et al., 2008)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by Mmp1^Q112stop/Mmp1²

(Miller et al., 2008)

Mmp2^W307stop has partially lethal - majority die | larval stage phenotype, enhanceable by Mmp1^Q112stop/Mmp1^Q112stop

(Page-McCaw et al., 2003)

NOT Enhanced by

Statement

Reference

Mmp2^W307stop has abnormal cell shape | embryonic stage phenotype, non-enhanceable by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has abnormal cell migration | embryonic stage phenotype, non-enhanceable by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Mmp1^Q112stop

(Miller et al., 2008)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Mmp1^Q112stop/Mmp1²

(Miller et al., 2008)

Suppressed by

Statement

Reference

Mmp2^Y53N/Mmp2^W307stop has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by arm²/arm[+]

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by dlp[+]/dlp²

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by dlp¹/dlp[+]

(Wang and Page-McCaw, 2014)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2008)

NOT suppressed by

Statement

Reference

Mmp2^W307stop has abnormal cell shape | embryonic stage phenotype, non-suppressible by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has abnormal cell migration | embryonic stage phenotype, non-suppressible by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Enhancer of

Statement

Reference

Mmp2[+]/Mmp2^W307stop is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of frac^Δ1

(Miller et al., 2011)

Mmp2^W307stop is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Mmp1^Q112stop

(Miller et al., 2008)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Mmp1^Q112stop/Mmp1²

(Miller et al., 2008)

Mmp2^W307stop/Mmp2²¹⁸ is an enhancer of partially lethal - majority die | larval stage phenotype of Mmp1^Q112stop/Mmp1²

(Page-McCaw et al., 2003)

Other

Statement

Reference

Mmp1^Q273stop, Mmp2^W307stop has abnormal neuroanatomy | pupal stage phenotype

(Kuo et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mmp2^Y53N/Mmp2^W307stop has stalk follicle cell | increased number | heat sensitive phenotype, enhanceable by dlp^UAS.cBa/Scer\GAL4^C587

(Wang and Page-McCaw, 2014)

Mmp2^W307stop has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, enhanceable by frac^Δ1/frac[+]

(Miller et al., 2011)

Mmp2^W307stop has larval segmental nerve branch SNa of A1-7 phenotype, enhanceable by Mmp1^Q112stop

(Miller et al., 2008)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 has larval segmental nerve branch SNa of A1-7 phenotype, enhanceable by Mmp1^Q112stop/Mmp1²

(Miller et al., 2008)

NOT Enhanced by

Statement

Reference

Mmp2^W307stop has heart primordium phenotype, non-enhanceable by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has embryonic heart cardioblast phenotype, non-enhanceable by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has filopodium | embryonic stage phenotype, non-enhanceable by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has lamellipodium | embryonic stage phenotype, non-enhanceable by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-enhanceable by Mmp1^Q112stop

(Miller et al., 2008)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-enhanceable by Mmp1^Q112stop/Mmp1²

(Miller et al., 2008)

Suppressed by

Statement

Reference

Mmp2^Y53N/Mmp2^W307stop has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by dlp¹/dlp[+]

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by dlp[+]/dlp²

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by arm²/arm[+]

(Wang and Page-McCaw, 2014)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 has larval intersegmental nerve branch ISNb of A1-7 phenotype, suppressible | partially by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2008)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 has larval segmental nerve branch SNa of A1-7 phenotype, suppressible | partially by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2008)

NOT suppressed by

Statement

Reference

Mmp2^W307stop has heart primordium phenotype, non-suppressible by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has embryonic heart cardioblast phenotype, non-suppressible by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has filopodium | embryonic stage phenotype, non-suppressible by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Mmp2^W307stop has lamellipodium | embryonic stage phenotype, non-suppressible by Mmp1^Q112stop/Mmp1^Q112stop

(Raza et al., 2017)

Enhancer of

Statement

Reference

Mmp2[+]/Mmp2^W307stop is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of frac^Δ1

(Miller et al., 2011)

Mmp2^W307stop is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of Mmp1^Q112stop

(Miller et al., 2008)

Mmp2^W307stop/Df(2R)Uba1-Mmp2 is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of Mmp1^Q112stop/Mmp1²

(Miller et al., 2008)

Other

Statement

Reference

Mmp1^Q273stop, Mmp2^W307stop has dendritic arborizing neuron | P-stage phenotype

(Kuo et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The heart developmental defects observed in Mmp1^Q112stop/Mmp1^Q112stop, Mmp2^W307stop/Mmp2^W307stop double mutants are similar to those seen in Mmp2^W307stop/Mmp2^W307stop single mutants.

(Raza et al., 2017)

The increase in average cell number in the stalks between egg chambers which is seen in Mmp2^Y53N/Mmp2^W307stop females after being shifted to the restrictive temperature is dominantly suppressed if the females also carry a single copy of arm², dlp¹ or dlp².

The increase in average cell number in the stalks between egg chambers which is seen in Mmp2^Y53N/Mmp2^W307stop females after being shifted to the restrictive temperature is enhanced if they are also expressing dlp^Scer\UAS.cBa under the control of Scer\GAL4^C587.

(Wang and Page-McCaw, 2014)

Mmp2^W307stop/+ ; frac^Δ1/+ double heterozygotes exhibit elevated levels of ISNb and SNa guidance defects. The incidence of ISNb mis-projection rises from 1% and 2% in frac^Δ1 and Mmp2^W307stop single heterozygotes, respectively, to 18% in the double heterozygote.

(Miller et al., 2011)

Mmp1^Q112stop,Mmp2^W307stop double mutant embryos show normal haemocyte migration into the tail region of the embryo.

(Siekhaus et al., 2010)

ISNb axon guidance defects in Mmp2^W307stop Mmp1^Q112stop or Mmp2^W307stop/Df(2R)Uba1-Mmp2 Mmp1^Q112stop/Mmp1² double mutants qualitatively and quantitatively mirror the phenotypes observed in the Mmp2 single mutants.

SNa axon defasciculation in Mmp2^W307stop Mmp1^Q112stop or Mmp2^W307stop/Df(2R)Uba1-Mmp2 Mmp1^Q112stop/Mmp1² double mutants is significantly increased relative to that of either single mutant.

(Miller et al., 2008)

Mmp1^Q273stop Mmp2^W307stop double mutant C4da clones not only exhibit dendritic branching patterns similar to wild-type clones during early pupariation, but also exhibit complete larval dendrite removal after head eversion at 20 hours after pupal formation as in wild-type controls.

(Kuo et al., 2005)

The larval lethality due to Mmp1^Q112stop/Mmp1² is enhanced by Mmp2²¹⁸/Mmp2^W307stop from 84% survival to 3rd instar and 7% pupariating to 54% survival to 3rd instar and 4% pupariating. The larval lethality of Mmp1^Q112stop; Mmp2^W307stop double homozygotes (73% survive to 2nd instar, 35% to 3rd instar, and none pupariate) is much greater than for either single homozygote.

(Page-McCaw et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Mmp2^W307stop is partially rescued by Scer\GAL4^Mef2.PU/Mmp2^UAS.cPa

(Raza et al., 2017)

Mmp2^W307stop/Mmp2²¹⁸ is partially rescued by Mmp2^GPI.EGFP

(Wang and Page-McCaw, 2014)

Comments

Expression of Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^Mef2.PU largely rescues the heart development defects (including cardioblast migration velocity, filopodial and lamellipodial activity, and lumen formation) seen in Mmp2^W307stop/Mmp2^W307stop mutant embryos.

(Raza et al., 2017)

Mmp2^{GPI.T:Avic\GFP-EGFP} completely rescues the pupal lethality of Mmp2 mutants. It does not rescue the lifespan, fertility and follicle cell defects of Mmp2^W307stop/Mmp2²¹⁸ flies.

(Wang and Page-McCaw, 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

frac is genetically downstream of Mmp2 in axon guidance.

(Miller et al., 2011)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Mmp2^W307*

(Yang et al., 2019, Jia et al., 2014, Wang and Page-McCaw, 2014, Miller et al., 2011, Glasheen et al., 2009, Miller et al., 2008, Kuo et al., 2005, Page-McCaw et al., 2003)

Mmp2^W307

(Guha et al., 2009)

Mmp2^W307stop

Mmp2^w307*

(Curtin et al., 2005)

mmp2^W307*

(Dear et al., 2016)

mmp2^W307

(Siekhaus et al., 2010)

mmp2^W307X

(Kanda et al., 2019)

mmp2^w307

(Raza et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (14)

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