FlyBase Allele Report: Dmel\Mmp1[Q273stop]

General Information

Symbol

Dmel\Mmp1^Q273stop

Species

D. melanogaster

Name

FlyBase ID

FBal0150758

Feature type

allele

Associated gene

Dmel\Mmp1

Associated Insertion(s)

Carried in Construct

Also Known As

Mmp1^Q273*

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Page-McCaw et al., 2003)

Mutagen

ethyl methanesulfonate

(Page-McCaw et al., 2003)

Progenitor genotype

Cytology

Description

Nonsense mutation leading to premature truncation of Mmp1 in the flexible hinge domain so that the predicted protein lacks the entire hemopexin domain.

(Glasheen et al., 2009)

Amino acid replacement: Q273term.

(Page-McCaw et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:24,675,815..24,675,815 [-]

Nucleotide change:

C24675815T

Amino acid change:

Reported amino acid change:

Q273term

Comment:

The current annotation uses an upstream AUG 29aa upstream relative to previous reports. This accounts for the discrepency in the reported vs. annotated amino acid coordinate of the mutation.

Comment:

Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.

(Page-McCaw et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage

(Kuo et al., 2005)

abnormal size | late third instar larval stage

(Glasheen et al., 2010)

decreased rate of movement | larval stage (with Mmp1^W439stop), with Mmp1^E225A.f1.UAS

(Glasheen et al., 2009)

lethal | pharate adult stage

(Page-McCaw et al., 2003)

lethal | pupal stage (with Mmp1²)

(Page-McCaw et al., 2003)

lethal - all die before end of pupal stage

(Glasheen et al., 2009, Kuo et al., 2005)

lethal - all die before end of pupal stage (with Mmp1^W439stop)

(Glasheen et al., 2009)

lethal - all die before end of pupal stage (with Mmp1^W439stop), with Mmp1^E225A.f1.UAS, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

lethal - all die before end of pupal stage (with Mmp1^W439stop), with Mmp1^{Pro-pex.f1.UAS}, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

partially lethal - majority die | larval stage

(Page-McCaw et al., 2003)

partially lethal - majority die | larval stage (with Mmp1²)

(Page-McCaw et al., 2003)

partially lethal - majority die | pupal stage

(Page-McCaw et al., 2003)

some die during pupal stage

(Glasheen et al., 2009, Kuo et al., 2005)

some die during pupal stage (with Mmp1^W439stop)

(Glasheen et al., 2009)

some die during pupal stage (with Mmp1^W439stop), with Mmp1^E225A.f1.UAS, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

some die during pupal stage (with Mmp1^W439stop), with Mmp1^{Pro-pex.f1.UAS}, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

Phenotype Manifest In

dendritic arborizing neuron | P-stage

(Kuo et al., 2005)

embryonic/larval dorsal tracheal branch | third instar larval stage

(Glasheen et al., 2009)

embryonic/larval dorsal tracheal branch | third instar larval stage (with Mmp1^W439stop)

(Glasheen et al., 2009)

embryonic/larval dorsal tracheal branch | third instar larval stage (with Mmp1^W439stop), with Mmp1^E225A.f1.UAS, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

head | pupal stage

(Glasheen et al., 2009)

head | pupal stage (with Mmp1^W439stop)

(Glasheen et al., 2009)

head | pupal stage (with Mmp1^W439stop), with Mmp1^E225A.f1.UAS, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

head | pupal stage (with Mmp1^W439stop), with Mmp1^{Pro-pex.f1.UAS}, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

mesothoracic tergum

(Page-McCaw et al., 2003)

taenidium | larval stage

(Glasheen et al., 2010)

wing | pupal stage (with Mmp1^W439stop)

(Glasheen et al., 2009)

wing | pupal stage (with Mmp1^W439stop), with Mmp1^E225A.f1.UAS, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

wing | pupal stage (with Mmp1^W439stop), with Mmp1^{Pro-pex.f1.UAS}, Scer\GAL4^αTub84B.PL

(Glasheen et al., 2009)

Detailed Description

Statement

Reference

Mmp1^Q273stop heterozygous mutants do not exhibit a delay in fat body cell dissociation during metamorphogenesis.

(Jia et al., 2014)

The intertaenidial distance in Mmp1^Q273stop late third instar larvae increases slightly from the beginning of the instar, but does not elongate fully compared to wild type, indicating that the hemopexin domain of Mmp1, missing in these mutants, is not necessary for taenidial expansion.

(Glasheen et al., 2010)

6% of Mmp1^Q273stop third instar larvae display broken tracheal dorsal trunks. 59% survive to pupariation. Dissected pupae show defects in head eversion.

6% of Mmp1^Q273stop/Mmp1^W439stop transheterozygous mutant third instar larvae display broken tracheal dorsal trunks. 78% survive to pupariation. A small number of dissected pupae show defects in head eversion.

Very few Mmp1^Q273stop/Mmp1^W439stop transheterozygous mutant third instar larvae expressing Mmp1^{Pro-pex.f1.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL display broken tracheal dorsal trunks. The majority of dissected pupae show defects in head eversion, and some animals show additional defects in wing eversion.

Expression of Mmp1^{E225A.f1.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL strongly enhances the phenotype of Mmp1^Q273stop/Mmp1^W439stop transheterozygous mutant third instar larvae. 15% display broken tracheal dorsal trunks and appear sluggish, and the majority of dissected pupae show defects in wing and head eversion. These phenotypes are stronger than either Mmp1^Q273stop/Mmp1^W439stop or Scer\GAL4^αTub84B.PL driving Mmp1^{E225A.f1.Scer\UAS} alone.

(Glasheen et al., 2009)

Mmp1^Q273stop mutants exhibit abundant C4da neuron larval dendrites after head eversion, with approximately 3% retained after head eversion. Whereas in wild-type pupae at 20hrs after pupal formation all larval dendrites from C4da neurons are cleared from the extracellular space, in Mmp1^Q273stop mutants, larval dendrites that are severed from the soma remain. These larval dendrites persist to much later stages at 50 hours after pupal formation, just before the lethal phase of Mmp1^Q273stop at midpupariation.

(Kuo et al., 2005)

94% of Mmp1^Q273stop mutant animals survive to 3rd instar, and 61% pupariate, but only 32% undergo head eversion, 26% form visible head bristles, and none eclose. Of the Mmp1^Q273stop mutant pupae that do not evert their heads, a small proportion (3/17) continue to develop adult body structures, including bristles, articulated legs, and abdominal cuticle stripes, appearing upon dissection from the pupal case as headless flies, often with cleft nota Dissection of the body cavities of these pharate adults reveals developing heads trapped inside. 87% of Mmp1^Q273stop/Mmp1² animals survive to third instar, but only 22% pupariate, 6% undergo head eversion, and none eclose.

(Page-McCaw et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Mmp1^Q273stop, Mmp2^A218V has abnormal developmental rate phenotype

(Jia et al., 2014)

Mmp1^Q273stop, Mmp2^W307stop has abnormal neuroanatomy | pupal stage phenotype

(Kuo et al., 2005)

Phenotype Manifest In

Other

Statement

Reference

Mmp1^Q273stop, Mmp2^A218V has fat body phenotype

(Jia et al., 2014)

Mmp1^Q273stop, Mmp2^W307stop has dendritic arborizing neuron | P-stage phenotype

(Kuo et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

A moderate delay in fat body cell dissociation during metamorphosis is seen in Mmp1^Q273stop Mmp2^A218V double heterozygotes.

(Jia et al., 2014)

Mmp1^Q273stop Mmp2^W307stop double mutant C4da clones not only exhibit dendritic branching patterns similar to wild-type clones during early pupariation, but also exhibit complete larval dendrite removal after head eversion at 20 hours after pupal formation as in wild-type controls.

(Kuo et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mmp1^Q273*

(Jia et al., 2014, Glasheen et al., 2010, Glasheen et al., 2009, Kuo et al., 2005, Page-McCaw et al., 2003)

Mmp1^Q273

(Sauerwald et al., 2019)

Mmp1^Q273stop

mmp1^Q273*

(Dear et al., 2016, Wang et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (8)

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